• Western blot analysis of extracts of various cell lines, using ARF4 antibody (STJ117836) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of C6 cells using ARF4 rabbit polyclonal antibody (STJ117836) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using ARF4 rabbit polyclonal antibody (STJ117836) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ARF4 antibody (1-180) (STJ117836)

SKU:
STJ117836

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ARF4 (1-180) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARF4
Gene ID: 378
Uniprot ID: ARF4_HUMAN
Immunogen Region: 1-180
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human ARF4 (NP_001651.1).
Immunogen Sequence: MGLTISSLFSRLFGKKQMRI LMVGLDAAGKTTILYKLKLG EIVTTIPTIGFNVETVEYKN ICFTVWDVGGQDRIRPLWKH YFQNTQGLIFVVDSNDRERI QEVADELQKMLLVDELRDAV LLLFANKQDLPNAMAISEMT DKLGLQSLRNRTWYVQATCA TQGTGLYEGLDWLSNELSKR
Function GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking.may modulate vesicle budding and uncoating within the Golgi apparatus. Part of the ciliary targeting complex containing Rab11, ASAP1, Rabin8/RAB3IP, RAB11FIP3 and ARF4, which direct preciliary vesicle trafficking to mother centriole and ciliogenesis initiation.
Protein Name Adp-Ribosylation Factor 4
Database Links Reactome: R-HSA-5620916
Reactome: R-HSA-6807878
Reactome: R-HSA-6811434
Cellular Localisation Golgi Apparatus
Membrane
Lipid-Anchor
Alternative Antibody Names Anti-Adp-Ribosylation Factor 4 antibody
Anti-ARF4 antibody
Anti-ARF2 antibody

Information sourced from Uniprot.org

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