• Western blot analysis of extracts of various cell lines, using ARFGAP1 antibody (STJ29198) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-ARFGAP1 antibody (1-220) (STJ29198)

SKU:
STJ29198

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ARFGAP1 (1-220) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARFGAP1
Gene ID: 55738
Uniprot ID: ARFG1_HUMAN
Immunogen Region: 1-220
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ARFGAP1 (NP_001268411.1).
Immunogen Sequence: MASPRTRKVLKEVRVQDENN VCFECGAFNPQWVSVTYGIW ICLECSGRHRGLGVHLSFVR SVTMDKWKDIELEKMKAGGN AKFREFLESQEDYDPCWSLQ EKYNSRAAALFRDKVVALAE GREWSLESSPAQNWTPPQPR TLPSMVHRVSGQPQSVTASS DKAFEDWLNDDLGSYQGAQG NRYVGFGNTPPPQKKEDDFL NNAMSSLYSGWSSFTTGASR
Function GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine.
Protein Name Adp-Ribosylation Factor Gtpase-Activating Protein 1
Arf Gap 1
Adp-Ribosylation Factor 1 Gtpase-Activating Protein
Arf1 Gap
Arf1-Directed Gtpase-Activating Protein
Database Links Reactome: R-HSA-381038
Reactome: R-HSA-6807878
Reactome: R-HSA-6811434
Reactome: R-HSA-8856828
Cellular Localisation Cytoplasm
Golgi Apparatus
Associates With The Golgi Complex
Alternative Antibody Names Anti-Adp-Ribosylation Factor Gtpase-Activating Protein 1 antibody
Anti-Arf Gap 1 antibody
Anti-Adp-Ribosylation Factor 1 Gtpase-Activating Protein antibody
Anti-Arf1 Gap antibody
Anti-Arf1-Directed Gtpase-Activating Protein antibody
Anti-ARFGAP1 antibody
Anti-ARF1GAP antibody

Information sourced from Uniprot.org

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