| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ARPC2 (1-300) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ARPC2 |
| Gene ID: |
10109 |
| Uniprot ID: |
ARPC2_HUMAN |
| Immunogen Region: |
1-300 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ARPC2 (NP_690601.1). |
| Immunogen Sequence: |
MILLEVNNRIIEETLALKFE NAAAGNKPEAVEVTFADFDG VLYHISNPNGDKTKVMVSIS LKFYKELQAHGADELLKRVY GSFLVNPESGYNVSLLYDLE NLPASKDSIVHQAGMLKRNC FASVFEKYFQFQEEGKEGEN RAVIHYRDDETMYVESKKDR VTVVFSTVFKDDDDVVIGKV FMQEFKEGRRASHTAPQVLF SHREPPLELKDTDAAVGDNI GYITFVLFPRHTNASARDN |
| Function | Actin-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. Seems to contact the mother actin filament. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs). |
| Protein Name | Actin-Related Protein 2/3 Complex Subunit 2Arp2/3 Complex 34 Kda SubunitP34-Arc |
| Database Links | Reactome: R-HSA-2029482Reactome: R-HSA-3928662Reactome: R-HSA-5663213Reactome: R-HSA-8856828Reactome: R-HSA-9664422 |
| Cellular Localisation | CytoplasmCytoskeletonCell ProjectionSynapseSynaptosomeNucleus |
| Alternative Antibody Names | Anti-Actin-Related Protein 2/3 Complex Subunit 2 antibodyAnti-Arp2/3 Complex 34 Kda Subunit antibodyAnti-P34-Arc antibodyAnti-ARPC2 antibodyAnti-ARC34 antibodyAnti-PRO2446 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89098/357557/STJ11100026_1__69013.1713121681.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")