• Immunoprecipitation analysis of 300 Mu g extracts from HepG2 cells using 3 Mu g ARPC2 antibody (STJ11102755). Western blot was performed from the immunoprecipitate using ARPC2 antibody (STJ11102755) at a dilution of 1:1000.
  • Immunofluorescence analysis of PC-12 cells using ARPC2 Rabbit monoclonal antibody (STJ11102755) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using ARPC2 Rabbit monoclonal antibody (STJ11102755) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using ARPC2 Rabbit monoclonal antibody (STJ11102755) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ARPC2 antibody (200-300) [S5MR] (STJ11102755)

SKU:
STJ11102755

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-ARPC2 (200-300) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARPC2
Gene ID: 10109
Uniprot ID: ARPC2_HUMAN
Immunogen Region: 200-300
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human ARPC2 (O15144).
Immunogen Sequence: FSHREPPLELKDTDAAVGDN IGYITFVLFPRHTNASARDN TINLIHTFRDYLHYHIKCSK AYIHTRMRAKTSDFLKVLNR ARPDAEKKEMKTITGKTFSS R
Function Actin-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. Seems to contact the mother actin filament. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).
Protein Name Actin-Related Protein 2/3 Complex Subunit 2
Arp2/3 Complex 34 Kda Subunit
P34-Arc
Database Links Reactome: R-HSA-2029482
Reactome: R-HSA-3928662
Reactome: R-HSA-5663213
Reactome: R-HSA-8856828
Reactome: R-HSA-9664422
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Projection
Synapse
Synaptosome
Nucleus
Alternative Antibody Names Anti-Actin-Related Protein 2/3 Complex Subunit 2 antibody
Anti-Arp2/3 Complex 34 Kda Subunit antibody
Anti-P34-Arc antibody
Anti-ARPC2 antibody
Anti-ARC34 antibody
Anti-PRO2446 antibody

Information sourced from Uniprot.org

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