Protein Localisation | Research Area | St John's Laboratory

Immunohistochemistry analysis of Heme Oxygenase 1 (HO-1/HMOX1) in paraffin-embedded mouse liver using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit monoclonal antibody (STJ11105129) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-HMOX1 antibody [S5129RM] (STJ11105129)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse

£126.50 - £554.50

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Anti-HO-1/HMOX1/HSP32 antibody (1-266aa) [AT1D6] (STJA0041828)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/FACS/ICC/IF

Reactivity :

Human

£219.50 - £332.50

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Western blot analysis of various lysates using HSCB Rabbit polyclonal antibody (STJ118406) at 1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-HSCB antibody (30-235) (STJ118406)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Anti-HSCB antibody (STJA0008832)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of HuvEc, K562 cells using 17 Beta-HSD4 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-HSD17B4 antibody (41-90 aa) (STJ96751)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-HSP60 antibody (1-573aa) [2E4] (STJA0041505)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF/IHC

Reactivity :

Human

£219.50 - £332.50

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Anti-Hsp60 antibody (N-Term) [M438] (STJA0041241)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

£579.50

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Anti-Hsp60 antibody [6C8-G1-B10] (STJA0032468)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-HSP60 antibody [HSP60-1] (STJ16102614)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB/IF/IHC

Reactivity :

Chicken/Cow/Dog/Hamster/Human/Monkey/Mouse/Pig/Rabbit/Rat/Sheep

£308.50 - £1,349.50

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Anti-Hsp60 antibody [R02-5B7] (STJA0034441)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Hsp60 antibody [R02-8D9] (STJA0034442)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Mouse/Rat

£133.50 - £281.50

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Anti-HSP60 antibody [SM5037] (STJA0005037)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£228.50

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Western blot analysis of extracts of various cell lines, using Grp75/MOT/HSPA9 Rabbit monoclonal antibody (STJ11101306) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-HSPA9 antibody (580-679) [S6MR] (STJ11101306)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-HSPA9/Mortalin/GRP75 antibody [SAA0812] (STJA0019624)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/IHC/IP/WB

Reactivity :

Human

£195.50 - £1,319.50

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Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells using 1 Mu g HSP60/HSPD1 antibody (STJ24105). Western blot was performed from the immunoprecipitate using HSP60/HSPD1 antibody (STJ24105) at a dilution of 1:1000.

Anti-HSPD1 antibody (27-240) (STJ24105)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Confocal imaging of HeLa cells using HSP60/HSPD1 Rabbit monoclonal antibody (STJ11101241, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Rabbit monoclonal antibody ( dilution 1:100) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

Anti-HSPD1 antibody (350-450) [S1MR] (STJ11101241)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Wheat

£118.50 - £521.50

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Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-HSP60 antibody. The HRP-conjugated anti- mouse IgG antibody was used to detect the antibody. Predicted band size: 60 kDa

Anti-HSPD1 antibody (500-573 aa) [ABT186] (STJ197164)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£180.00 - £261.00

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Immunofluorescence analysis of Hela cell. 1, HSP60 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-HSPD1 antibody (511-560 aa) (STJ93621)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot of HeLa cell lysate showing specific immunolabeling of the ~60 kDa HSP60 protein.

Anti-HSPD1 antibody (Full-Length) [1C7] (STJA0003845)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Feline/Goat/Guinea Pig/Hamster/Horse/Non-Human Primates/Rabbit/Sheep/Zebrafish

£488.50

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Anti-HSPD1 antibody (N-Term) [M438] (STJA0005440)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£390.50

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Western blot analysis using HSP60 monoclonal antibody against T47D (1) , HeLa (2) , HepG2 (3) , A549 (4) , Jurkat (5) , HEK293 (6) , NIH/3T3 (7) , PC-12 (8) and Cos7 (9) cell lysate.

Anti-HSPD1 antibody [3G8] (STJ98142)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£149.00 - £360.00

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Western blot detection of Hsp60 in Hela, Raji, Jurkat, C6, C2C12 and 3T3 cell lysates using Hsp60 mouse mAb (1:1000 diluted).Predicted band size:60KDa.Observed band size:60KDa.

Anti-HSPD1 antibody [6C8-G1-B10] (STJ99241)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IP

Reactivity :

Human/Mouse/Rat

£149.00 - £360.00

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Anti-Hydroxyacid Oxidase 1 antibody [4B4-3F7-9A6] (STJA0032829)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Mouse/Rat

£133.50 - £281.50

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Anti-Hydroxyacid Oxidase 1 antibody [ZN117] (STJA0033360)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£214.50 - £308.50

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Western blot analysis of extracts of various cell lines, using Insulin-degrading enzyme (Insulin-degrading enzyme (IDE) ) Rabbit monoclonal antibody (STJ11101287) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-IDE antibody (1-100) [S7MR] (STJ11101287)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunofluorescence analysis of A549 cells using Insulin-degrading enzyme (Insulin-degrading enzyme (IDE) ) antibody (STJ24120). Blue: DAPI for nuclear staining.

Anti-IDE antibody (1-250) (STJ24120)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£126.50 - £618.50

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Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Insulin Degrading Enzyme/IDE antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-IDE antibody (56-69 aa) [R07-6D3] (STJA0012660)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human

£133.50 - £281.50

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Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1, IDE monoclonal antibody (3H4) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-IDE antibody [3H4] (STJ96985)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Hamster

£49.00 - £261.00

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Anti-IDE antibody [PT1295R] (STJA0040300)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-IDE antibody [SAA1582] (STJA0020010)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£198.50 - £1,170.50

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Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells, using 3 Mu g IDH1 antibody (STJ115211). Western blot was performed from the immunoprecipitate using IDH1 antibody (STJ115211) at a dilution of 1:1000.

Anti-IDH1 antibody (1-414) (STJ115211)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunofluorescence analysis of PC-12 cells using IDH1 Rabbit polyclonal antibody (STJ24121) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-IDH1 antibody (100-414) (STJ24121)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£110.50 - £505.50

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Western blot analysis of 293, HeLa cells using IDH1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IDH1 antibody (N-Term) (STJ96781)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-IDH1 antibody [3G9] (STJA0032656)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

£224.50 - £308.50

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Anti-IDH1 antibody [BP6205] (STJA0037708)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£244.50 - £335.50

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Anti-IDH1 antibody [R05-2G5] (STJA0033790)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-IDH1 antibody [R07-9M3] (STJA0037177)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-IDH1 antibody [SQab22257] (STJ16103006)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£708.50

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Anti-IDH1 antibody [SRM13624] (STJ11108354)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-IDH1 antibody [SRM14726] (STJ11109456)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Formalin-fixed, paraffin-embedded human glioma stained with anti-IHD1 antibody using peroxidase-conjugate and DAB chromogen. Note nuclear and cytoplasmic staining of infiltrating glioma cells whereas normal glial cells are negative

Anti-IDH1 antibody [ZR7] (STJ180518)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£186.50 - £792.50

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Anti-IDH1-R132G-specific antibody [GMab-1] (STJA0022479)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

£195.50 - £1,319.50

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Anti-IDH1-R132H-specific antibody [HMab-1] (STJA0022481)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

£195.50 - £1,319.50

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Anti-IDH1-R132L-specific antibody [LMab-1] (STJA0022480)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

£195.50 - £1,319.50

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Anti-IDH1-R132S-specific antibody [SMab-1] (STJA0022482)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

£195.50 - £1,319.50

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-IDH3G antibody (STJ193072)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Confocal imaging of RAW264. 7 LPS and RAW264. 7 cells using iNOS Rabbit monoclonal antibody (STJ11103535, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-iNOS antibody (2-200) [S5MR] (STJ11103535)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£118.50 - £521.50

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Anti-iNOS antibody (C-Term) (STJA0041295)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

£474.50

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