Anti-iNOS antibody (C-Term) [M398] (STJA0041289)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2a
ImmunogenClone (M398) was generated from a recombinant protein that included amino acid residues within the C-terminal region of human iNOS. The human iNOS sequence used has high homology with similar regions in rat and mouse iNOS.
General Information
| Short Description | Mouse monoclonal anti-iNOS (C-Term) for use in ELISA, WB and ICC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/WB/ICC |
| Host | Mouse |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | M398 |
| Isotype | IgG2a |
| Conjugation | Unconjugated |
| Purification | Protein A Purified |
| Dilution Range | WB 1:250ICC 1:50 |
| Formulation | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Instruction | Storage at-20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at-20°C. |
Target Information
| Gene Symbol | NOS2 |
| Gene ID | 4843 |
| Uniprot ID | NOS2_HUMAN |
| Immunogen | Clone (M398) was generated from a recombinant protein that included amino acid residues within the C-terminal region of human iNOS. The human iNOS sequence used has high homology with similar regions in rat and mouse iNOS. |
| Immunogen Region | C-Term |
| Immunogen Sequence | Human |
| Specificity | The antibody detects a 130 kDa* protein on SDS-PAGE immunoblots of mouse macrophages (RAW264.7) treated with IFN Gamma and LPS, or J774A.1 cells treated with LPS only. |
Additional Info
| Tissue Specificity | Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. Expressed in chondrocytes. |
| Post Translational Modifications | Polyubiquitinated.mediated by SPSB1, SPSB2 and SPSB4, leading to proteasomal degradation. |
| Function | Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8. |
| Protein Name | Nitric Oxide Synthase - InducibleHepatocyte NosHep-NosInducible No SynthaseInducible NosInosNos Type IiPeptidyl-Cysteine S-Nitrosylase Nos2 |
| Database Links | Reactome: R-HSA-1222556Reactome: R-HSA-392154Reactome: R-HSA-6785807Reactome: R-HSA-9033241Reactome: R-HSA-9636249 |
| Cellular Localisation | CytoplasmCytosolLocalizes As Discrete Foci Scattered Throughout The Cytosol And In The Presence Of Spsb1 And Spsb4Exhibits A More Diffuse Cytosolic Localization |
| Alternative Antibody Names | Anti-Nitric Oxide Synthase - Inducible antibodyAnti-Hepatocyte Nos antibodyAnti-Hep-Nos antibodyAnti-Inducible No Synthase antibodyAnti-Inducible Nos antibodyAnti-Inos antibodyAnti-Nos Type Ii antibodyAnti-Peptidyl-Cysteine S-Nitrosylase Nos2 antibodyAnti-NOS2 antibodyAnti-NOS2A antibody |
Information sourced from Uniprot.org