Metabolism | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using NNMT antibody (STJ115965) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NNMT antibody (1-264) (STJ115965)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of A-549 cells, using NNMT antibody (STJ28756) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-NNMT antibody (1-264) (STJ28756)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-NNMT antibody (STJA0006970)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Anti-NNT antibody (240-320 aa) (STJ191019)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of various lysates using NNT Rabbit polyclonal antibody (STJ116248) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-NNT antibody (881-1086) (STJ116248)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of human placenta cells using eNOS Rabbit polyclonal antibody (STJ24784) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-NOS3 antibody (1000-1203) (STJ24784)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of human placenta cells using eNOS Rabbit polyclonal antibody (STJ11104517) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-NOS3 antibody (1035-1110) (STJ11104517)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

$151.88 - $708.08

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Immunohistochemistry analysis of eNOS in paraffin-embedded rat spleen tissue using eNOS Rabbit monoclonal antibody (STJ11104147) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-NOS3 antibody (1035-1110) [S4147RM] (STJ11104147)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Immunofluorescence analysis of Hela cell. 1, NOS3 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-NOS3 antibody (1145-1194) (STJ94539)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunofluorescence analysis of HUVEC cells using eNOS Rabbit polyclonal antibody (STJ117269) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NOS3 antibody (639-738) (STJ117269)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-NOS3 antibody (C-Term) (STJA0005490)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$431.33

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Immunohistochemistry analysis of paraffin-embedded human lymph node (left) and colon cancer (right) tissues with DAB staining using NOS3 monoclonal antibody.

Anti-NOS3 antibody [6H2] (STJ98282)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/ELISA

Reactivity :

Human

$194.40 - $469.80

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Western blot analysis of lysates from 1) Rat Heart Tissue, 2) huvec, 3) Jurkat cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta Polyclonal Antibody (cat: (STJ91464) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-NOS3 antibody [Mix] (STJ97066)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Rabbit

$64.80 - $340.20

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Western blot analysis of various lysates, using eNOS antibody (STJ11104808) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-NOS3 antibody [S4808RM] (STJ11104808)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Western blot analysis of lysates from wild type (WT) and NOSIP knockout (KO) 293T cells using [KO Validated] NOSIP Rabbit polyclonal antibody (STJ11104394) at 1:500 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-NOSIP antibody (1-301) (STJ11104394)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$151.88 - $708.08

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Western blot analysis of extracts of various cell lines, using NOSIP antibody (STJ112064) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NOSIP antibody (1-301) (STJ112064)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$151.88 - $708.08

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-NOSIP antibody (220-300 aa) (STJ191087)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Immunofluorescence analysis of U-2 OS cells using NOSTRIN Rabbit polyclonal antibody (STJ118383) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NOSTRIN antibody (270-430) (STJ118383)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from MDA-MB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-NOSTRIN antibody (309-359 aa) (STJ195841)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of extracts from rat stomach, 293 cells, using NPAS2 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-NPAS2 antibody (340-420 aa) (STJ96410)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

$64.80 - $340.20

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Immunofluorescence analysis of C6 cells using NPAS2 Rabbit polyclonal antibody (STJ119239) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NPAS2 antibody (45-240) (STJ119239)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of various lysates, using NQO1 antibody (STJ11101714) at 1:20000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NQO1 antibody (1-100) [S4MR] (STJ11101714)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Western blot analysis of lysates from HeLa cells, using NQO1 Rabbit pAb (STJ24804) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-NQO1 antibody (1-274) (STJ24804)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of L929 cells using NQO1 Rabbit polyclonal antibody (STJ24805) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NQO1 antibody (1-274) (STJ24805)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various cells using NQO1 Polyclonal Antibody diluted at 1：2000

Anti-NQO1 antibody (203-252) (STJ94551)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using NQO2 Rabbit monoclonal antibody (STJ11101959) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NQO2 antibody (1-100) [S9MR] (STJ11101959)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Western blot analysis of various lysates using NQO2 Rabbit polyclonal antibody (STJ113336) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NQO2 antibody (1-231) (STJ113336)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Immunohistochemistry analysis of NQO2 in paraffin-embedded Mouse kidney using NQO2 Rabbit polyclonal antibody (STJ113339) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-NQO2 antibody (1-231) (STJ113339)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Western blot analysis of extracts of HeLa cells, using NQO2 antibody (STJ27393) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s.

Anti-NQO2 antibody (1-231) (STJ27393)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$151.88 - $708.08

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-NQO2 antibody (40-120 aa) (STJ191344)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Immunofluorescence analysis of PC-12 cells using Rev-Erb Alpha/NR1D1 Rabbit polyclonal antibody (STJ11100546) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NR1D1 antibody (280-360) (STJ11100546)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-NR1D1 antibody (350-430 aa) (STJ95423)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Immunocytochemistry analysis of NR1D1 (green) in Hela using NR1D1 antibody, and DAPI (blue)

Anti-NR1D1 antibody (591-605 aa) [R05-8B1] (STJA0011699)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse/Rat

$332.78

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Western blot analysis of HeLa, using LXR beta/NER antibody (STJ11104867) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-NR1H2 antibody (1-100) (STJ11104867)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of NIH/3T3 cells using NR1H2 Rabbit polyclonal antibody (STJ11104520) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NR1H2 antibody (180-260) (STJ11104520)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Western blot analysis of various lysates using NR1H2 Rabbit polyclonal antibody (STJ118723) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-NR1H2 antibody (180-260) (STJ118723)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-NR1H2 antibody (STJ192129)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Confocal imaging of C2C12 cells using LXR Alpha Rabbit monoclonal antibody (STJ11101976, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-NR1H3 antibody (1-100) [S6MR] (STJ11101976)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Western blot analysis of HeLa cells using LXR Alpha Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-NR1H3 antibody (151-200 aa) (STJ96508)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Hamster

$64.80 - $340.20

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Western blot analysis of lysates from Mouse kidney, using LXR Alpha Rabbit polyclonal antibody (STJ24810) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NR1H3 antibody (160-230) (STJ24810)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of Rat liver, using NR1H4 Rabbit polyclonal antibody (STJ114657) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-NR1H4 antibody (1-270) (STJ114657)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

$151.88 - $708.08

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Anti-NR1H4 antibody (1-50) (STJ501969)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

$577.13

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Anti-NR1H4 antibody (1-50) {Biotin} (STJ501970)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

$713.48

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Anti-NR1H4 antibody (1-50) {FITC} (STJ501971)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

$713.48

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Western blot analysis of various lysates using NR1H4 Rabbit pAb (STJ110618) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-NR1H4 antibody (207-476) (STJ110618)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Huang, Jianbo, et al. "Effects of mulberry leaf on experimental hyperlipidemia rats induced by high‑fat diet. " Experimental and therapeutic medicine 16. 2 (2018) : 547-556.

Anti-NR1H4 antibody (STJ192265)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-NRF1 antibody (1-300) (STJ116123)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of 3T3 cells using NRF-1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-NRF1 antibody (221-270) (STJ94553)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Bovine

$64.80 - $340.20

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