Anti-IQGAP1 antibody (247-296 aa) (STJ93752)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human IQGAP1 at the amino acid range 247-296
General Information
| Short Description | Rabbit polyclonal anti-Ras GTPase-activating-like protein IQGAP1 (247-296 aa) for use in WB and IHC in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IHC-P 1:50-300 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | IQGAP1 |
| Gene ID | 8826 |
| Uniprot ID | IQGA1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human IQGAP1 at the amino acid range 247-296 |
| Immunogen Region | 247-296 aa |
| Specificity | IQGAP1 Polyclonal Antibody detects endogenous levels of IQGAP1 protein. |
Additional Info
| Post Translational Modifications | Phosphorylation of Ser-1443 by PKC/PRKCE prevents interaction between C1 and C2, allowing binding of nucleotide-free CDC42. Ser-1443 phosphorylation enhances the ability to promote neurite outgrowth. |
| Function | Plays a crucial role in regulating the dynamics and assembly of the actin cytoskeleton. Recruited to the cell cortex by interaction with ILK which allows it to cooperate with its effector DIAPH1 to locally stabilize microtubules and allow stable insertion of caveolae into the plasma membrane. Binds to activated CDC42 but does not stimulate its GTPase activity. Associates with calmodulin. May promote neurite outgrowth. May play a possible role in cell cycle regulation by contributing to cell cycle progression after DNA replication arrest. |
| Protein Name | Ras Gtpase-Activating-Like Protein Iqgap1P195 |
| Database Links | Reactome: R-HSA-373753Reactome: R-HSA-381676Reactome: R-HSA-5626467Reactome: R-HSA-5674135Reactome: R-HSA-6798695Reactome: R-HSA-6802946Reactome: R-HSA-6802948Reactome: R-HSA-6802952Reactome: R-HSA-6802955Reactome: R-HSA-8980692Reactome: R-HSA-9013106Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013406Reactome: R-HSA-9013420Reactome: R-HSA-9013424Reactome: R-HSA-9649948Reactome: R-HSA-9656223 |
| Cellular Localisation | Cell MembraneNucleusCytoplasmCell CortexApical Cell MembraneBasolateral Cell MembraneSubcellular Distribution Is Regulated By The Cell CycleNuclear Levels Increase At G1/S Phase |
| Alternative Antibody Names | Anti-Ras Gtpase-Activating-Like Protein Iqgap1 antibodyAnti-P195 antibodyAnti-IQGAP1 antibodyAnti-KIAA0051 antibody |
Information sourced from Uniprot.org