Metabolism Of Proteins | Research Area | St John's Laboratory

Western blot analysis of various lysates, using [KO Validated] Beclin 1 Rabbit pAb (STJ29492) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-BECN1 antibody (1-450) (STJ29492)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from 1) mouse-brain, 2) mouse-lung, 3) Hela cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） GAPDH monoclonal antibody (2B8) (cat: (STJ96931) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-BECN1 antibody (110-190 aa) (STJ98594)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Chicken

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using Beclin-1 Mouse mAb diluted at 1:200.

Anti-BECN1 antibody (110-190 aa) [5A11] (STJ97769)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-BECN1 antibody (110-190 aa) [5C2] (STJ97761)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse/Cow

£48.00 - £252.00

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Anti-BECN1 antibody (110-190 aa) [5C5] (STJ97770)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using Beclin 1 Rabbit polyclonal antibody (STJ11101202) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-BECN1 antibody (25-450) (STJ11101202)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis using Beclin-1 monoclonal antibody against Mouse brain, A431, HeLa cell lysate.

Anti-BECN1 antibody (STJ98472)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Pig/Rabbit

£144.00 - £348.00

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Western blot analysis using Beclin-1 monoclonal antibody against HeLa (1) , A431 (2) , MCF-7 (3) , RAJI (4) , Jurkat (5) and SKBR-3 (6) cell lysate.

Anti-BECN1 antibody [2A4] (STJ97862)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot detection of Beclin 1 in Hela, 3T3, C6, COS7, CHO-K1, Rat brain and Jurkat cell lysates using Beclin 1 mouse mAb (1:1000 diluted).Predicted band size:52KDa.Observed band size:55KDa.

Anti-BECN1 antibody [4F4-B10-C9] (STJ99148)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat/Monkey/Hamster

£144.00 - £348.00

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Western blot of wild type (WT) beclin-1 expressed in HeLa cell lysate showing specific immunolabeling of beclin-1 phosphorylated at Ser295. This immunolabeling is eliminated in the beclin-1 alanine substitution mutant (S295A: Mut) expressed in HeLa cell lysate.

Anti-BECN1-Ser295 antibody (STJA0003836)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/Human/Non-Human Primates/Xenopus/Zebrafish

£454.50

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Western blot analysis of various lysates using BET1 Rabbit polyclonal antibody (STJ115036) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-BET1 antibody (1-94) (STJ115036)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of various lysates using BET1L Rabbit polyclonal antibody (STJ113867) at 1:3000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s.

Anti-BET1L antibody (1-60) (STJ113867)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-BET1L antibody (STJA0008432)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from wild type (WT) and Î²2-microglobulin knockdown (KD) HeLa cells using [KD Validated] beta 2 Microglobulin Rabbit mAb (STJ11107311) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 60s.

Anti-beta 2 Microglobulin antibody (21-119 aa) [SRM] (STJ11107311)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Anti-Beta 2-microglobulin antibody (40-119 aa) [SRM] (STJ11106042)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of Beta-catenin. Sample: Protein treated by RIPA Lysis Buffer. Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:3000, RT, 1h.

Anti-beta Catenin antibody [SM4939] (STJA0004939)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Western blot analysis of various lysates, using Beta III-Tubulin Rabbit pAb (STJ11106048) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Beta III-Tubulin antibody (40-260 aa) (STJ11106048)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/FC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using Beta III-Tubulin Mouse mAb (STJ11106837) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Beta III-Tubulin antibody (40-260 aa) [SMM] (STJ11106837)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Anti-Beta-2 microglobulin antibody [EBS-O-109] (STJ16101034)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/IHC

Reactivity :

Human

£287.50 - £1,324.50

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Western blot analysis of various lysates using Beta-Actin Rabbit pAb (STJ11103684) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Beta-Actin antibody (1-375) (STJ11103684)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£135.50 - £249.50

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Western blot analysis of lysates from HeLa cells, using Beta-Actin Mouse mAb (STJ11103683) at 1:2000-1:640000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Beta-Actin antibody [S3MR] (STJ11103683)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£135.50 - £249.50

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Immunohistochemistry analysis of Beta-Catenin in paraffin-embedded human skin using Beta-Catenin Mouse monoclonal antibody (STJ11103229) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-Beta-Catenin antibody (300-400 aa) [S9MM] (STJ11103229)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-Beta-Catenin antibody [14] (STJ16100314)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£989.50 - £1,291.50

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Anti-Beta-Catenin antibody [17C2] (STJ16101680)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£1,228.50

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Western blot analysis of Rat lung, using Osteocalcin (BGLAP) rabbit polyclonal antibody (STJ116843) at 1:1500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-BGLAP antibody (1-100) (STJ116843)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of U-87MG cells, using Osteocalcin (BGLAP) antibody (STJ27961) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-BGLAP antibody (1-100) (STJ27961)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using Osteocalcin (BGLAP) antibody (STJ11100198) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-BGLAP antibody (1-95) (STJ11100198)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-BGLAP antibody (31-80 aa) (STJ192217)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemistry of rat tissue with Anti-BGLAP primary antibody (STJ502017) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-BGLAP antibody (50-100) (STJ502017)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Pig/Rat

£427.50

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Anti-BGLAP antibody (50-100) {Biotin} (STJ502018)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Pig/Rat

£528.50

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Anti-BGLAP antibody (50-100) {FITC} (STJ502019)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Pig/Rat

£528.50

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Western blot analysis of extracts of various cell lines, using cIAP1 antibody (STJ11101767) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-BIRC2 antibody (1-100) [S7MR] (STJ11101767)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Rat

£146.50 - £642.50

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Western blot analysis of lysates from Jurkat cells, using cIAP1 Rabbit pAb (STJ22800) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 90s.

Anti-BIRC2 antibody (1-300) (STJ22800)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunofluorescence analysis of BALA-3T3 cells using cIAP1 rabbit polyclonal antibody (STJ114903) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-BIRC2 antibody (331-618) (STJ114903)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-BIRC2 antibody (STJ193113)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-BIRC2 antibody [PT0955R] (STJA0039810)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£0.00

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Immunofluorescence analysis of C6 cells using cIAP2 Rabbit polyclonal antibody (STJ117997) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-BIRC3 antibody (100-604) (STJ117997)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot detection of c-IAP2 in Ramos, COS7, Raji and Daudi cell lysates using c-IAP2 mouse mAb (1:1000 diluted).Predicted band size: 68KDa, Observed band size:72KDa.

Anti-BIRC3 antibody [1F12-E5-G12] (STJ99165)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Monkey

£144.00 - £348.00

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Western blot analysis of extracts of various cell lines, using Survivin antibody (STJ11101752) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-BIRC5 antibody (1-100) [S2MR] (STJ11101752)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£129.50 - £574.50

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Western blot analysis of extracts of various cell lines, using Survivin Rabbit monoclonal antibody (STJ11102164) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.

Anti-BIRC5 antibody (1-140) [S4MR] (STJ11102164)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£129.50 - £574.50

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Immunofluorescence analysis of MCF7 cells using Survivin Rabbit polyclonal antibody (STJ11105318) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-BIRC5 antibody (1-142) (STJ11105318)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded mouse fetal liver using Survivin Rabbit polyclonal antibody (STJ26172) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-BIRC5 antibody (1-142) (STJ26172)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using Survivin antibody (STJ11104675) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-BIRC5 antibody (1-142) [S4675RM] (STJ11104675)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Survivin Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-BIRC5 antibody (11-60 aa) (STJ95837)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of Hela cell. 1, Survivin Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Active Caspase-3 monoclonal antibody (5E1) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-BIRC5 antibody (86-135 aa) (STJ95838)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-BIRC5 antibody (C-Term) (STJA0005747)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£455.50

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Anti-BIRC5 antibody (STJ503164)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1, Survivin monoclonal antibody (Mix) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-BIRC5 antibody [Mix] (STJ97436)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat

£48.00 - £252.00

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