Metabolism Of Proteins | Research Area | St John's Laboratory

Western blot analysis of various lysates using alpha 1 Spectrin Rabbit mAb (STJ11102499) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-SPTA1 antibody (2300-2400) [S9MR] (STJ11102499)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

Choose Options

Immunofluorescence analysis of paraffin-embedded mouse lung using alpha 1 Spectrin Rabbit polyclonal antibody (STJ114233) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SPTA1 antibody (940-1160) (STJ114233)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Immunofluorescence analysis of U-2 OS cells using Alpha Fodrin Rabbit polyclonal antibody (STJ114871) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SPTAN1 antibody (950-1130) (STJ114871)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Anti-SPTAN1 antibody (STJA0005593)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£367.50

Choose Options

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~240 kDa alpha II spectrin protein.

Anti-SPTAN1 antibody [3D7] (STJA0003570)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC

Reactivity :

Bovine/Human/Mouse/Rat

£454.50

Choose Options

Western blot analysis of various lysates using SPTB Rabbit pAb (STJ115015) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 90s.

Anti-SPTB antibody (2080-2180) (STJ115015)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of various lysates using SPTBN1 Rabbit polyclonal antibody (STJ25683) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SPTBN1 antibody (2100-2200) (STJ25683)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

Choose Options

Western blot analysis of lysates from COLO cells, using SPTBN1 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-SPTBN1 antibody (651-700 aa) (STJ95746)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-SPTBN2 antibody (644-694 aa) (STJ195669)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat

£48.00 - £252.00

Choose Options

Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-SPTBN4 antibody (STJ191461)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

Choose Options

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using SPTBN5 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-SPTBN5 antibody (481-530 aa) (STJ95747)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

Choose Options

Immunofluorescence analysis of HepG2 cells using SQSTM1/p62 Rabbit polyclonal antibody (STJ110069) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SQSTM1 antibody (1-440) (STJ110069)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Immunoprecipitation analysis of 100 Mu g extracts of HepG2 cells using 3 Mu g SQSTM1/p62 antibody (STJ113162). Western blot was performed from the immunoprecipitate using SQSTM1/p62 antibody (STJ113162) at a dilution of 1:1000.

Anti-SQSTM1 antibody (1-440) (STJ113162)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Immunoprecipitation analysis of 200 Mu g extracts of C6 cells using 3 Mu g SQSTM1/p62 antibody (STJ113746). Western blot was performed from the immunoprecipitate using SQSTM1/p62 antibody (STJ113746) at a dilution of 1:1000.

Anti-SQSTM1 antibody (1-440) (STJ113746)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Rat

£129.50 - £642.50

Choose Options

Anti-SQSTM1 antibody (300-360 aa) (STJ13101971)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human

£331.50

Choose Options

Anti-SQSTM1 antibody (300-360 aa) (STJ13101972)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

Choose Options

Anti-SQSTM1 antibody (300-360 aa) (STJ13102022)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

Choose Options

Anti-SQSTM1 antibody (300-360 aa) (STJ13102023)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human

£331.50

Choose Options

Immunofluorescence analysis of U2OS cells using SQSTM1/p62 Rabbit polyclonal antibody (STJ11104488) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SQSTM1 antibody (340-440) (STJ11104488)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Western blot analysis of lysates from HeLa cells, using SQSTM1/p62 Rabbit polyclonal antibody (STJ113744) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-SQSTM1 antibody (341-440) (STJ113744)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

Choose Options

Anti-SQSTM1 antibody (341-440) [S3MR] (STJ11101773)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

Choose Options

Immunohistochemistry of rat tissue with Anti-SQSTM1 primary antibody (STJ503125) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-SQSTM1 antibody (400-440) (STJ503125)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

Choose Options

Anti-SQSTM1 antibody (400-440) {Biotin} (STJ503126)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

Choose Options

Anti-SQSTM1 antibody (400-440) {FITC} (STJ503127)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

Choose Options

Western blot analysis of lysates from AD293 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-SQSTM1 antibody (STJ195471)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Western blot analysis of various lysates using SRP14 Rabbit polyclonal antibody (STJ114792) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SRP14 antibody (1-136) (STJ114792)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-SRP14 antibody (25-75 aa) (STJ194604)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

Choose Options

Immunohistochemistry analysis of paraffin-embedded mouse brain using SRP19 antibody (STJ28835) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-SRP19 antibody (1-144) (STJ28835)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Anti-SRP19 antibody (22-72) (STJA0006673)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

Choose Options

Western blot analysis of extracts of various cell lines, using SRP54 antibody (STJ25695) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SRP54 antibody (196-455) (STJ25695)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

Choose Options

Immunofluorescence analysis of U-2 OS cells using SRP54 Rabbit monoclonal antibody (STJ11103481) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SRP54 antibody (400-504) [S1MR] (STJ11103481)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

Choose Options

Anti-SRP54 antibody (STJ191418)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

Choose Options

Anti-SRP68 antibody (STJA0007042)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

Choose Options

Western blot analysis of extracts of various cell lines, using SRP72 antibody (STJ11102732) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-SRP72 antibody (440-544) (STJ11102732)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of extracts of A-431 cells, using SRP9 antibody (STJ25697) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SRP9 antibody (1-86) (STJ25697)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

Choose Options

Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-SRP9 antibody (7-57 aa) (STJ196214)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Mouse

£48.00 - £252.00

Choose Options

Western blot analysis of extracts of various cell lines, using SRPR antibody (STJ27735) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SRPR antibody (1-220) (STJ27735)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

Choose Options

Anti-SRPRA antibody (STJA0008269)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

Choose Options

Anti-SRPRB antibody (174-224) (STJA0006303)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Immunofluorescence analysis of L929 cells using SRPRB Rabbit polyclonal antibody (STJ112604) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-SRPRB antibody (57-271) (STJ112604)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Immunoprecipitation analysis of 200 Mu g extracts of MCF7 cells using 1 Mu g SSBP1 antibody (STJ29067). Western blot was performed from the immunoprecipitate using SSBP1 antibody (STJ29067) at a dilution of 1:1000.

Anti-SSBP1 antibody (1-148) (STJ29067)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

Choose Options

Western blot analysis of various cells using SSBP1 Polyclonal Antibody

Anti-SSBP1 antibody (99-148 aa) (STJ95791)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Cow

£48.00 - £252.00

Choose Options

Western blot analysis of various lysates using SSR1 Rabbit pAb (STJ111212) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 20s.

Anti-SSR1 antibody (19-207) (STJ111212)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

Choose Options

Anti-SSR1 antibody (STJA0007020)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-SSR2 antibody (107-157 aa) (STJ194315)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

Choose Options

Immunofluorescence analysis of NIH/3T3 cells using SSR2 Rabbit polyclonal antibody (STJ11103317) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SSR2 antibody (18-145) (STJ11103317)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of various lysates using SSR3 Rabbit polyclonal antibody (STJ119884) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SSR3 antibody (70-145) (STJ119884)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-SSR3 antibody (STJ192529)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options