• Western blot analysis of lysates from 293 cells treated with serum, using AFX (Phospho-Ser197) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using AFX (Phospho-Ser197) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-FoxO4 (S197) Polyclonal Antibody

Anti-Phospho-FOXO4-Ser197 antibody (164-213 aa) (STJ90276)

SKU:
STJ90276

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Forkhead box protein O4-Ser197 (164-213 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FOXO4
Gene ID: 4303
Uniprot ID: FOXO4_HUMAN
Immunogen Region: 164-213 aa
Specificity: Phospho-FoxO4 (S197) Polyclonal Antibody detects endogenous levels of FoxO4 protein only when phosphorylated at S197.
Immunogen: The antiserum was produced against synthesized peptide derived from the human AFX around the phosphorylation site of Ser197 at the amino acid range 164-213
Post Translational Modifications Acetylation by CREBBP/CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity. Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization. May be phosphorylated at multiple sites by NLK. Monoubiquitinated.monoubiquitination is induced by oxidative stress and reduced by deacetylase inhibitors.results in its relocalization to the nucleus and its increased transcriptional activity. Deubiquitinated by USP7.deubiquitination is induced by oxidative stress.enhances its interaction with USP7 and consequently, deubiquitination.increases its translocation to the cytoplasm and inhibits its transcriptional activity. Hydrogene-peroxide-induced ubiquitination and USP7-mediated deubiquitination have no major effect on its protein stability.
Function Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity.
Protein Name Forkhead Box Protein O4
Fork Head Domain Transcription Factor Afx1
Database Links Reactome: R-HSA-198693
Reactome: R-HSA-5674400
Reactome: R-HSA-5689880
Reactome: R-HSA-9614399
Reactome: R-HSA-9614657
Reactome: R-HSA-9615017
Reactome: R-HSA-9617629
Reactome: R-HSA-9617828
Reactome: R-HSA-9733709
Cellular Localisation Cytoplasm
Nucleus
When Phosphorylated
Translocated From Nucleus To Cytoplasm
Dephosphorylation Triggers Nuclear Translocation
Monoubiquitination Increases Nuclear Localization
When Deubiquitinated
Alternative Antibody Names Anti-Forkhead Box Protein O4 antibody
Anti-Fork Head Domain Transcription Factor Afx1 antibody
Anti-FOXO4 antibody
Anti-AFX antibody
Anti-AFX1 antibody
Anti-MLLT7 antibody

Information sourced from Uniprot.org

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