Immune System | Research Area | St John's Laboratory

Western blot analysis of various lysates using Bim Rabbit monoclonal antibody (STJ11101775) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-BCL2L11 antibody (1-100) [S5MR] (STJ11101775)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various cells using Bim Polyclonal Antibody

Anti-BCL2L11 antibody (1-50 aa) (STJ91855)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Immunofluorescence analysis of U2OS cells using Bim Rabbit polyclonal antibody (STJ118216) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-BCL2L11 antibody (100-198) (STJ118216)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded human liver using BCL2L11 antibody (STJ114428) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-BCL2L11 antibody (30-130) (STJ114428)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunofluorescence analysis of A549 cells using BCL6 antibody (STJ29253).

Anti-BCL6 antibody (1-280) (STJ29253)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-BCL6 antibody (200-250) (STJ500226)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£427.50

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Anti-BCL6 antibody (200-250) {Biotin} (STJ500224)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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Anti-BCL6 antibody (200-250) {FITC} (STJ500225)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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Immunofluorescence analysis of human-stomach tissue. 1, Bcl-6 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-BCL6 antibody (271-320 aa) (STJ96676)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 293 cells using Bcl-6 Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-BCL6 antibody (299-348 aa) (STJ91839)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry of baboon spleen with Anti-BCL6 primary antibody (STJ500223) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-BCL6 antibody (50-100) (STJ500223)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£427.50

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Anti-BCL6 antibody (50-100) {Biotin} (STJ500221)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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Anti-BCL6 antibody (50-100) {FITC} (STJ500222)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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Immunohistochemistry of mouse spleen with Anti-BCL6 primary antibody (STJ500219) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-BCL6 antibody (610-660) (STJ500219)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£427.50

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Anti-BCL6 antibody (610-660) {Biotin} (STJ500227)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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Anti-BCL6 antibody (610-660) {FITC} (STJ500228)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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Immunohistochemistry of rat spleen with Anti-BCL6 primary antibody (STJ500220) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-BCL6 antibody (650-700) (STJ500220)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Rat

£427.50

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Anti-BCL6 antibody (650-700) {Biotin} (STJ500229)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Rat

£528.50

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Anti-BCL6 antibody (650-700) {FITC} (STJ500230)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human

£528.50

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Anti-Beclin1 antibody (STJ13100044)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£331.50

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Anti-Beclin1 antibody (STJ13100051)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat/Marmoset

£331.50

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Western blot analysis of Beclin 1 in K562, rat Brain, C6, 3T3, Hela lysates using Beclin 1 antibody.

Anti-BECN1 antibody (1-200 aa) [R02-8D5] (STJA0010822)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£246.50

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Immunofluorescence analysis of PC-12 cells using [KO Validated] Beclin 1 Rabbit polyclonal antibody (STJ11104484) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-BECN1 antibody (1-280) (STJ11104484)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of extracts of various cell lines, using Beclin 1 rabbit polyclonal antibody (STJ113348) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-BECN1 antibody (1-280) (STJ113348)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using Beclin 1 antibody (STJ29492) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-BECN1 antibody (1-450) (STJ29492)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from 1) mouse-brain, 2) mouse-lung, 3) Hela cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） GAPDH monoclonal antibody (2B8) (cat: (STJ96931) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-BECN1 antibody (110-190 aa) (STJ98594)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Chicken

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using Beclin-1 Mouse mAb diluted at 1:200.

Anti-BECN1 antibody (110-190 aa) [5A11] (STJ97769)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-BECN1 antibody (110-190 aa) [5C2] (STJ97761)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse/Cow

£48.00 - £252.00

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Anti-BECN1 antibody (110-190 aa) [5C5] (STJ97770)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using Beclin 1 Rabbit polyclonal antibody (STJ11101202) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-BECN1 antibody (25-450) (STJ11101202)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis using Beclin-1 monoclonal antibody against Mouse brain, A431, HeLa cell lysate.

Anti-BECN1 antibody (STJ98472)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Pig/Rabbit

£144.00 - £348.00

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Western blot analysis using Beclin-1 monoclonal antibody against HeLa (1) , A431 (2) , MCF-7 (3) , RAJI (4) , Jurkat (5) and SKBR-3 (6) cell lysate.

Anti-BECN1 antibody [2A4] (STJ97862)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot detection of Beclin 1 in Hela, 3T3, C6, COS7, CHO-K1, Rat brain and Jurkat cell lysates using Beclin 1 mouse mAb (1:1000 diluted).Predicted band size:52KDa.Observed band size:55KDa.

Anti-BECN1 antibody [4F4-B10-C9] (STJ99148)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat/Monkey/Hamster

£144.00 - £348.00

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Western blot of wild type (WT) beclin-1 expressed in HeLa cell lysate showing specific immunolabeling of beclin-1 phosphorylated at Ser295. This immunolabeling is eliminated in the beclin-1 alanine substitution mutant (S295A: Mut) expressed in HeLa cell lysate.

Anti-BECN1-Ser295 antibody (STJA0003836)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/Human/Non-Human Primates/Xenopus/Zebrafish

£454.50

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Anti-beta 2 Microglobulin antibody (21-119 aa) [SRM] (STJ11107311)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Anti-Beta 2-microglobulin antibody (40-119 aa) [SRM] (STJ11106042)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of Beta-catenin. Sample: Protein treated by RIPA Lysis Buffer. Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:3000, RT, 1h.

Anti-beta Catenin antibody [SM4939] (STJA0004939)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Western blot analysis of extracts of various cell lines, using beta glucuronidase (beta glucuronidase (GUSB) ) antibody (STJ11102980) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-beta glucuronidase antibody (1-100) [S0MR] (STJ11102980)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Anti-Beta III-Tubulin antibody (40-260 aa) (STJ11106048)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/FC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-Beta III-Tubulin antibody (40-260 aa) [SMM] (STJ11106837)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Anti-beta Tubulin antibody (C-Term) (STJ13100428)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Rat/Human/Xenopus/Zebrafish

£331.50

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Anti-Beta-2 microglobulin antibody [EBS-O-109] (STJ16101034)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/IHC

Reactivity :

Human

£287.50 - £1,324.50

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Western blot analysis of extracts of various cell lines, using Beta-Actin antibody (STJ11103684) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Beta-Actin antibody (1-375) (STJ11103684)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£135.50 - £249.50

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Western blot analysis of extracts of various cell lines, using Beta-Actin antibody (STJ11103683) at 1:20000 dilution. Secondary antibody: HRP Goat Anti-mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Beta-Actin antibody [S3MR] (STJ11103683)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£135.50 - £249.50

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Immunohistochemistry analysis of Beta-Catenin in paraffin-embedded human skin using Beta-Catenin Mouse monoclonal antibody (STJ11103229) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-Beta-Catenin antibody (300-400 aa) [S9MM] (STJ11103229)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-Beta-Catenin antibody [14] (STJ16100314)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£989.50 - £1,291.50

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Anti-Beta-Catenin antibody [17C2] (STJ16101680)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£1,228.50

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Western blot analysis of extracts of various cell lines, using Beta-Tubulin antibody (STJ11103686) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Beta-Tubulin antibody (40-260) (STJ11103686)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC

Reactivity :

Human/Mouse/Rat

£135.50 - £249.50

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