Immune System | Research Area | St John's Laboratory

Immunofluorescence analysis of U2OS cells using EGR1 Rabbit polyclonal antibody (STJ23496) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-EGR1 antibody (1-250) (STJ23496)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from ECV-304 cells, using EGR1 Rabbit pAb (STJ29407) at 1:1000 dilution. ECV-304 cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-EGR1 antibody (1-250) (STJ29407)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £618.50

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Western blot analysis using Egr-1 monoclonal antibody against EGR1-hIgGFc transfected HEK293 (1) cell lysate.

Anti-EGR1 antibody (282-433 aa) [8A6] (STJ98014)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£149.00 - £360.00

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Anti-EGR1 antibody [PT0584R] (STJA0039249)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£285.00 - £540.00

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EGR1 antibody western blot 293T cells STJA0035476

Anti-EGR1 antibody [R29-4U-9] (STJA0035476)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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ICC IF of EIF2S1 in HeLa cells stained with STJA0041610 antibody and Alexa Fluor 488

Anti-eIF-2 alpha/EIF2S1 antibody (1-315aa) [AT5E10] (STJA0041610)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF

Reactivity :

Human

£219.50 - £332.50

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eIF2-alpha antibody western blot K562 COS7 3T3 C2C12 MCF-7 C6 HeLa STJA0032482

Anti-eIF2A antibody [2E4-F5-E7] (STJA0032482)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of various lysates, using PKR/EIF2AK2 Rabbit polyclonal antibody (STJ27547) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 3s.

Anti-EIF2AK2 antibody (1-270) (STJ27547)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Western blot analysis of various cells using PKR Polyclonal Antibody diluted at 1：2000

Anti-EIF2AK2 antibody (413-462 aa) (STJ95144)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using PKR/EIF2AK2 antibody (STJ11101690) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-EIF2AK2 antibody (450-551) [S0MR] (STJ11101690)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Immunofluorescence analysis of MCF7 cells using PERK Rabbit polyclonal antibody (STJ11105581) at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-EIF2AK3 antibody (50-600) (STJ11105581)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Immunofluorescence analysis of NIH/3T3 cells using PERK Rabbit polyclonal antibody (STJ11100155) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-EIF2AK3 antibody (867-1116) (STJ11100155)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of Hela cell. 1, PERK Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-EIF2AK3 antibody (947-996 aa) (STJ95031)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of CACO2 lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-EIF2AK3 antibody (STJ99624)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis using PERK monoclonal antibody against HEK293 (1) and EIF2AK3-hIgGFc transfected HEK293 (2) cell lysate.

Anti-EIF2AK3 antibody [5G5] (STJ98319)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£149.00 - £360.00

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Immunohistochemistry analysis of paraffin-embedded human lung cancer using eIF2C3 Rabbit monoclonal antibody (STJ11103159) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-eIF2C3 antibody (721-860) [S9MR] (STJ11103159)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of various lysates, using eIF2 Alpha antibody (STJ23509) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-EIF2S1 antibody (1-260) (STJ23509)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of Hela cell. 1, eIF2 Alpha Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Caspase 9 monoclonal antibody (3-20) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-EIF2S1 antibody (21-70 aa) (STJ92872)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey/Fish

£49.00 - £261.00

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Western blot detection of eIF2 Alpha in K562, COS7, 3T3, C2C12, MCF-7, C6 and Hela cell lysates using eIF2 Alpha mouse mAb (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa.

Anti-EIF2S1 antibody [2E4-F5-E7] (STJ99266)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IP

Reactivity :

Human/Mouse/Rat

£149.00 - £360.00

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Western blot analysis of various lysates using EIF2S2 Rabbit polyclonal antibody (STJ11102835) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-EIF2S2 antibody (1-175) (STJ11102835)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4° overnight STJ92873

Anti-EIF2S2 antibody (10-90 aa) (STJ92873)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of the lysates from HT-29 cells using EIF2S2 antibody.

Anti-EIF2S2 antibody (61-110 aa) (STJ92874)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded rat brain using EIF2S3 antibody (STJ28664) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-EIF2S3 antibody (1-250) (STJ28664)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of rat brain, using EIF2S3 antibody (STJ23510) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EIF2S3 antibody (173-472) (STJ23510)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£110.50 - £505.50

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Anti-EIF2S3 antibody (STJA0007805)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-EIF4A1 antibody (1-100) (STJ27247)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-EIF4A1 antibody [11B8] (STJ16103463)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

£746.50

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Anti-EIF4A1 antibody [3F11] (STJ16103527)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

£746.50

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eIF4A1 antibody western blot T47D 3T3 COS7 C6 HeLa STJA0032806

Anti-eIF4A1 antibody [6D7-10D5-7H7] (STJA0032806)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Immunohistochemical analysis of paraffin-embedded Human-colon-cancer tissue. 1, eIF4A1 monoclonal antibody (M8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-EIF4A1 antibody [M8] (STJ97038)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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eIF4A1 antibody western blot MCF-7 lysates STJA0036850

Anti-eIF4A1 antibody [R02-4P3] (STJA0036850)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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eIF4A1 antibody western blot K562 C6 3T3 HeLa STJA0034267

Anti-eIF4A1 antibody [R04-2K6] (STJA0034267)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-eIF4A1 antibody [SRM14653] (STJ11109383)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£118.50 - £521.50

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Western blot analysis of extracts of various cell lines, using EIF4A2 antibody (STJ29906) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-EIF4A2 antibody (1-270) (STJ29906)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot eIF4A2 protein 46 kDa detection in mouse testes lysate STJA0003625

Anti-EIF4A2 antibody (N-Term) (STJA0003625)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Bovine/Non-Human Primates/Rat

£488.50

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Anti-EIF4A2 antibody (STJA0007013)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates using eIF4A3 Rabbit polyclonal antibody (STJ23515) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-EIF4A3 antibody (1-210) (STJ23515)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-EIF4A3 antibody (218-268 aa) (STJ194381)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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eIF4A3 antibody western blot HeLa NFDM STJA0036848

Anti-eIF4A3 antibody [R03-7Z4] (STJA0036848)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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eIF4A3 antibody western blot HeLa CHO-K1 C6 STJA0034268

Anti-eIF4A3 antibody [R05-1B7] (STJA0034268)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Rat/Hamster

£133.50 - £281.50

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Anti-eIF4A3 antibody [SRM13640] (STJ11108370)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of various lysates, using eIF4E antibody (STJ23516) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Anti-EIF4E antibody (1-100) (STJ23516)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunofluorescence analysis of MCF7 cells using eIF4E antibody (STJ114889). Blue: DAPI for nuclear staining.

Anti-EIF4E antibody (1-217) (STJ114889)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£110.50 - £505.50

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Immunoprecipitation analysis of 600 Mu g extracts of Mouse testis using 3 Mu g eIF4E antibody (STJ11101582). Western blot was performed from the immunoprecipitate using eIF4E (STJ11101582) at a dilution of 1:500.

Anti-EIF4E antibody (118-217) [S2MR] (STJ11101582)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Mouse/Rat

£126.50 - £554.50

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Western blot analysis of various cells using eIF4E Polyclonal Antibody diluted at 1：1000

Anti-EIF4E antibody (168-217 aa) (STJ92881)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Anti-eIF4E antibody (STJA0037908)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£187.50 - £281.50

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Western blot analysis using eIF4E monoclonal antibody against HeLa (1) , HEK293 (2) and K562 (3) cell lysate.

Anti-EIF4E antibody [5D11] (STJ98016)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£149.00 - £360.00

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eIF4E antibody western blot MDA-MB-468 ZR751 MCF-7 T47D CHO-K1 STJA0032481

Anti-eIF4E antibody [8C8-C10-C12] (STJA0032481)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

Choose Options