• Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-EIF4A3 antibody (218-268 aa) (STJ194381)

SKU:
STJ194381

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Eukaryotic initiation factor 4A-III (218-268 aa) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EIF4A3
Gene ID: 9775
Uniprot ID: IF4A3_HUMAN
Immunogen Region: 218-268 aa
Specificity: This antibody detects endogenous levels of IF4A3 at Human/Mouse/Rat
Immunogen: Synthesized peptide derived from the human IF4A3 at the amino acid range 218-268
Function ATP-dependent RNA helicase. Involved in pre-mRNA splicing as component of the spliceosome. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH-RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH-RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes).specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S).the function is different from the established EJC assembly. Involved in craniofacial development.
Protein Name Eukaryotic Initiation Factor 4a-Iii
Eif-4a-Iii
Eif4a-Iii
Atp-Dependent Rna Helicase Ddx48
Atp-Dependent Rna Helicase Eif4a-3
Dead Box Protein 48
Eukaryotic Initiation Factor 4a-Like Nuk-34
Eukaryotic Translation Initiation Factor 4a Isoform 3
Nuclear Matrix Protein 265
Nmp 265
Hnmp 265 Cleaved Into - Eukaryotic Initiation Factor 4a-Iii - N-Terminally Processed
Database Links Reactome: R-HSA-1169408
Reactome: R-HSA-159236
Reactome: R-HSA-429947
Reactome: R-HSA-72163
Reactome: R-HSA-72187
Reactome: R-HSA-73856
Reactome: R-HSA-9010553
Reactome: R-HSA-975957
Cellular Localisation Nucleus
Nucleus Speckle
Cytoplasm
Nucleocytoplasmic Shuttling Protein
Travels To The Cytoplasm As Part Of The Exon Junction Complex (Ejc) Bound To Mrna
Detected In Dendritic Layer As Well As The Nuclear And Cytoplasmic (Somatic) Compartments Of Neurons
Colocalizes With Stau1 And Fmr1 In Dendrites
Alternative Antibody Names Anti-Eukaryotic Initiation Factor 4a-Iii antibody
Anti-Eif-4a-Iii antibody
Anti-Eif4a-Iii antibody
Anti-Atp-Dependent Rna Helicase Ddx48 antibody
Anti-Atp-Dependent Rna Helicase Eif4a-3 antibody
Anti-Dead Box Protein 48 antibody
Anti-Eukaryotic Initiation Factor 4a-Like Nuk-34 antibody
Anti-Eukaryotic Translation Initiation Factor 4a Isoform 3 antibody
Anti-Nuclear Matrix Protein 265 antibody
Anti-Nmp 265 antibody
Anti-Hnmp 265 Cleaved Into - Eukaryotic Initiation Factor 4a-Iii - N-Terminally Processed antibody
Anti-EIF4A3 antibody
Anti-DDX48 antibody
Anti-KIAA0111 antibody

Information sourced from Uniprot.org

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