Anti-EIF4A1 antibody (1-100) (STJ27247)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal EIF4A1 (1-100) antibody for use in WB, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:5000IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirement |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EIF4A1 |
| Gene ID | 1973 |
| Uniprot ID | IF4A1_HUMAN |
| Immunogen Region | 1-100 |
| Immunogen Sequence | MSASQDSRSRDNGPDGMEPE GVIESNWNEIVDSFDDMNLS ESLLRGIYAYGFEKPSAIQQ RAILPCIKGYDVIAQAQSGT GKTATFAISILQQIELDLKA |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human eIF4A1 (NP_001407.1). |
Additional Info
| Function | ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. As a result, promotes cell proliferation and growth. |
| Protein Name | Eukaryotic Initiation Factor 4a-IEif-4a-IEif4a-IAtp-Dependent Rna Helicase Eif4a-1 |
| Database Links | Reactome: R-HSA-1169408Reactome: R-HSA-156827Reactome: R-HSA-429947Reactome: R-HSA-72649Reactome: R-HSA-72662Reactome: R-HSA-72702Reactome: R-HSA-72706Reactome: R-HSA-9820841Reactome: R-HSA-9820865 |
| Cellular Localisation | CytoplasmPerinuclear RegionCell MembraneStress GranuleColocalizes With Pkp1 In Stress Granules Following Arsenate Or Hydrogen Peroxide Treatment |
| Alternative Antibody Names | Anti-Eukaryotic Initiation Factor 4a-I antibodyAnti-Eif-4a-I antibodyAnti-Eif4a-I antibodyAnti-Atp-Dependent Rna Helicase Eif4a-1 antibodyAnti-EIF4A1 antibodyAnti-DDX2A antibodyAnti-EIF4A antibody |
Information sourced from Uniprot.org