• Western blot analysis of extracts of various cell lines, using EIF4A2 antibody (STJ29906) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-EIF4A2 antibody (1-270) (STJ29906)

SKU:
STJ29906

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EIF4A2 (1-270) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EIF4A2
Gene ID: 1974
Uniprot ID: IF4A2_HUMAN
Immunogen Region: 1-270
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human EIF4A2 (NP_001958.2).
Immunogen Sequence: MSGGSADYNREHGGPEGMDP DGVIESNWNEIVDNFDDMNL KESLLRGIYAYGFEKPSAIQ QRAIIPCIKGYDVIAQAQSG TGKTATFAISILQQLEIEFK ETQALVLAPTRELAQQIQKV ILALGDYMGATCHACIGGTN VRNEMQKLQAEAPHIVVGTP GRVFDMLNRRYLSPKWIKMF VLDEADEMLSRGFKDQIYEI FQKLNTSIQVVLLSATMPTD VLEVTKKFMRDPIRILVKK
Function ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
Protein Name Eukaryotic Initiation Factor 4a-Ii
Eif-4a-Ii
Eif4a-Ii
Atp-Dependent Rna Helicase Eif4a-2
Database Links Reactome: R-HSA-1169408
Reactome: R-HSA-156827
Reactome: R-HSA-429947
Reactome: R-HSA-72649
Reactome: R-HSA-72662
Reactome: R-HSA-72702
Reactome: R-HSA-72706
Alternative Antibody Names Anti-Eukaryotic Initiation Factor 4a-Ii antibody
Anti-Eif-4a-Ii antibody
Anti-Eif4a-Ii antibody
Anti-Atp-Dependent Rna Helicase Eif4a-2 antibody
Anti-EIF4A2 antibody
Anti-DDX2B antibody
Anti-EIF4F antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance