Haemostasis | Research Area | St John's Laboratory

Western blot analysis of various lysates, using PP2A Catalytic Alpha antibody (STJ28785) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PPP2CA antibody (1-309) (STJ28785)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various cells using PP2A-C Alpha Polyclonal Antibody diluted at 1：2000

Anti-PPP2CA antibody (260-309 aa) (STJ95195)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunocytochemistry analysis of PP2A alpha/beta (green) in hela using PP2A alpha/beta antibody, and DAPI (blue).

Anti-PPP2CA antibody (277-294 aa) [R04-4G4] (STJA0011815)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat/Hamster

£246.50

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Western blot analysis using PP2A-C Alpha monoclonal antibody against MCF7 cell lysate.

Anti-PPP2CA antibody (N-Term) (STJ98535)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human/Mouse/Cow/Chicken/Dog/Pig/Rabbit/Zebrafish

£149.00 - £360.00

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Immunofluorescence analysis of U-2 OS cells using PP2A Catalytic Beta antibody (STJ25086) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-PPP2CB antibody (1-309) (STJ25086)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot detection of PP2A alpha and beta in Hela, MCF7, C6 and 3T3 cell lysates using PP2A alpha and beta mouse mAb (1:2000 diluted).Predicted band size:36KDa.Observed band size:36KDa.

Anti-PPP2CB antibody [2G2-H6-G11-E8] (STJ99088)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£149.00 - £360.00

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Immunofluorescence analysis of U2OS cells using PP2A-Aalpha/PR65 Alpha/PP2A-A Alpha/PR65 Alpha/PPP2R1A antibody (STJ28363). Blue: DAPI for nuclear staining.

Anti-PPP2R1A antibody (1-270) (STJ28363)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-PPP2R1A antibody (500-580 aa) (STJ190194)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from Rat spleen using PP2A-A Beta/PR65 Beta/PPP2R1B Rabbit polyclonal antibody (STJ11105479) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Anti-PPP2R1B antibody (502-601) (STJ11105479)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£110.50 - £505.50

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Western blot analysis of various cells using PP2A-A Beta Polyclonal Antibody

Anti-PPP2R1B antibody (552-601 aa) (STJ95191)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of the lysates from HeLa cells using PPP2R5A antibody.

Anti-PPP2R5A antibody (321-370 aa) (STJ95193)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-PPP2R5B antibody (160-240 aa) (STJ191381)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from Rat brain using PP2A-B56 Beta/PR61 Beta/PPP2R5B Rabbit polyclonal antibody (STJ11103926) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PPP2R5B antibody (400-497) (STJ11103926)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using PP2A-B56 Gamma/PR61 Gamma/PP2A-B56 Gamma/PR61 Gamma/PPP2R5C antibody (STJ27433) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PPP2R5C antibody (1-320) (STJ27433)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-PPP2R5C antibody (360-440 aa) (STJ191383)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of U-2 OS cells using PP2A-B56 Delta/PR61 Delta/PPP2R5D Rabbit polyclonal antibody (STJ118153) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PPP2R5D antibody (450-550) (STJ118153)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of 293T cells using PP2A-B56-Delta Polyclonal Antibody diluted at 1：1000

Anti-PPP2R5D antibody (544-593 aa) (STJ95194)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-PPP2R5E antibody (150-230 aa) (STJ191382)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from Rat spleen using PP2A-B56 Delta/PR61 Delta/PPP2R5E Rabbit polyclonal antibody (STJ11103927) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-PPP2R5E antibody (50-150) (STJ11103927)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£110.50 - £505.50

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Anti-PR3 antibody [PRG-2] (STJ16100777)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/ELISA/WB

Reactivity :

Human

£662.50

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Western blot analysis of lysates from rat kidney, using PRCP Rabbit pAb (STJ25100) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-PRCP antibody (407-496) (STJ25100)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£110.50 - £505.50

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Anti-PRCP antibody (STJ192089)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-PRCP antibody (STJ193169)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates using PKA C-alpha (PRKACA) Rabbit polyclonal antibody (STJ111027) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PRKACA antibody (1-351) (STJ111027)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates, using PKAC Alpha Rabbit polyclonal antibody (STJ11105351) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PRKACA antibody (251-351) (STJ11105351)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunocytochemistry analysis of cAMP Protein Kinase Catalytic subunit (green) in Hela using cAMP Protein Kinase Catalytic subunit antibody, and DAPI (blue)

Anti-PRKACA antibody (319-332 aa) [R07-8H3] (STJA0011064)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£246.50

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Anti-PRKACA antibody [PT0438R] (STJA0039556)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Western blot analysis of lysates from wild type (WT) and PKA C-alpha (PRKACA) knockdown (KD) 293T cells, using [KD Validated] PKA C-alpha (PRKACA) Mouse monoclonal antibody (STJ11104590) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PRKACA antibody [S4590MM] (STJ11104590)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of Rat spleen, using PKA C-beta (PRKACB) antibody (STJ27277) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PRKACB antibody (250-351) (STJ27277)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from Jurkat and COLO205 cells, using KAPCB Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-PRKACB antibody (291-340 aa) (STJ95115)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of extracts of Rat brain, using PRKACG antibody (STJ110026) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-PRKACG antibody (1-351) (STJ110026)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of COLO205 cells using PKA Gamma cat Polyclonal Antibody

Anti-PRKACG antibody (1-50 aa) (STJ95116)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of PRKACG in mouse testis, mouse lung lysates using PKA C gamma antibody.

Anti-PRKACG antibody (338-351 aa) [R01-8I1] (STJA0011829)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£264.50

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Western blot analysis of extracts from normal (control) and PRKAR1A knockout (KO) 293T cells, using PRKAR1A antibody (STJ25122) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-PRKAR1A antibody (1-250) (STJ25122)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various cells using PKA I Alpha reg Polyclonal Antibody

Anti-PRKAR1A antibody (271-320 aa) (STJ95111)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using PKA I Beta reg Polyclonal Antibody

Anti-PRKAR1B antibody (106-155 aa) (STJ95112)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of L929 cells using [KO Validated] PKA RII Alpha (PRKAR2A) /PKR2 Rabbit polyclonal antibody (STJ25123) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PRKAR2A antibody (1-404) (STJ25123)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of extracts of various cell lines, using PKA RII Alpha (PRKAR2A) Rabbit monoclonal antibody (STJ11101964) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-PRKAR2A antibody (312-404) [S4MR] (STJ11101964)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of 293 cells using PKA II Alpha reg Polyclonal Antibody

Anti-PRKAR2A antibody (41-90 aa) (STJ95109)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded rat ovary using PRKAR2B Rabbit monoclonal antibody (STJ11103108) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-PRKAR2B antibody (319-418) [S8MR] (STJ11103108)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Mouse/Rat

£118.50 - £521.50

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Western blot analysis of various cells using PKA II Beta reg Polyclonal Antibody

Anti-PRKAR2B antibody (79-128 aa) (STJ95110)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates, using PKC alpha Rabbit monoclonal antibody (STJ11105041) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-PRKCA antibody (200-300 aa) [S5041RM] (STJ11105041)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Whole cell lysates were separated by 15% SDS-PAGE, and the membrane was blotted with anti-PKC (PTR1190) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HelaPredicted band size: 70kDaObserved band size: 80kDa

Anti-PRKCA antibody (450-550) [PTR1190] (STJA0006229)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Rabbit/Bovine

£49.00 - £261.00

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Western blot analysis of various lysates, using PKC alpha Rabbit polyclonal antibody (STJ115305) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PRKCA antibody (573-672) (STJ115305)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded human colon using PKC alpha antibody (STJ25125) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-PRKCA antibody (573-672) (STJ25125)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g PKC alpha antibody (STJ112958). Western blot was performed from the immunoprecipitate using PKC alpha antibody (STJ112958) at a dilution of 1:1000.

Anti-PRKCA antibody (573-672) [S8MR] (STJ112958)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of NIH-3T3 cells using PKC Alpha Polyclonal Antibody

Anti-PRKCA antibody (606-655 aa) (STJ95119)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded Human colon cancer using PKC alpha antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-PRKCA antibody (658-672 aa) [R05-4E5] (STJA0013069)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat

£264.50

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