Gene Expression And Transcription | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using E2F4 antibody (STJ11103078) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-E2F4 antibody (250-350) [S8MR] (STJ11103078)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Western blot analysis of Jurkat cells using E2F-4 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-E2F4 antibody (61-110 aa) (STJ96687)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-E2F4 antibody (89-198) (STJ11103181)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£110.50 - £505.50

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E2F4 antibody immunohistochemistry paraffin-embedded endometrial tissue STJA0037740

Anti-E2F4 antibody [R08-5M7] (STJA0037740)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£308.50

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Anti-E2F4 antibody [R09-7E6] (STJA0034261)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£140.50 - £308.50

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Anti-E2F4 antibody [SQab30318] (STJ16103030)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£708.50

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Western blot analysis of HeLa cells using E2F-5 Polyclonal Antibody

Anti-E2F5 antibody (93-142 aa) (STJ92810)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Chromatin immunoprecipitation analysis of extracts of 293T cells, using E2F6 antibody (STJ116186) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-E2F6 antibody (1-281) (STJ116186)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA/ChIP

Reactivity :

Human/Mouse

£110.50 - £505.50

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CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K562 cells with 1 Mu g E2F6 rabbit monoclonal antibody (STJ11101356) , along with a Goat Anti-rabbit IgG (H+L). The CUT&Tag results indicate the enrichment pattern of E2F6 in representative gene loci (BRCA1) , as shown in figure.

Anti-E2F6 antibody (110-281) [S6MR] (STJ11101356)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA/ChIP/CUT&Tag

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of various cells using E2F-6 Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-E2F6 antibody (141-190 aa) (STJ92811)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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E2F6 antibody western blot K562 lysates STJA0036816

Anti-E2F6 antibody [R09-2A4] (STJA0036816)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/FC

Reactivity :

Human

£133.50 - £281.50

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Anti-E2F7 antibody (560-640 aa) (STJ191781)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates using E2F8 Rabbit polyclonal antibody (STJ23459) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.

Anti-E2F8 antibody (788-867) (STJ23459)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Anti-E2F8 antibody (STJ191922)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates using EAF1 Rabbit polyclonal antibody (STJ119827) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-EAF1 antibody (50-120) (STJ119827)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£110.50 - £505.50

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Anti-EAF1 antibody (STJA0007298)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-EAF2 antibody (STJA0009552)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of U-2 OS cells using EED Rabbit polyclonal antibody (STJ114646) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-EED antibody (1-100) (STJ114646)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates, using EED rabbit polyclonal antibody (STJ27324) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-EED antibody (1-400) (STJ27324)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-EED antibody (STJA0006915)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-EED IP antibody [SAA0245] (STJA0018760)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/IP/IP-MS

Reactivity :

Human

£195.50 - £1,319.50

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Western blot analysis of various lysates, using EGF Receptor (E746-A750del Specific) Rabbit pAb (STJ11106934) at 1:400 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-EGF Receptor-E746-A750del Specific antibody (STJ11106934)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Western blot analysis of A431, using EGFR rabbit polyclonal antibody (STJ113093) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-EGFR antibody (1021-1210) (STJ113093)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of A-431 cells using [KD Validated] EGFR Rabbit polyclonal antibody (STJ113840) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-EGFR antibody (1021-1210) (STJ113840)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

£126.50 - £618.50

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Immunocytochemistry analysis of EGFR (green) in 3T3 using EGFR antibody, and DAPI (blue).

Anti-EGFR antibody (1075-1093 aa) [R01-2D3] (STJA0012677)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse

£140.50 - £308.50

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Immunocytochemistry analysis of EGFR (green) in Hela using EGFR antibody, and DAPI (blue)

Anti-EGFR antibody (1075-1093 aa) [R03-6B1] (STJA0013018)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse

£140.50 - £308.50

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Anti-EGFR antibody (1075-1093 aa) [R09-7I5] (STJA0011260)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

£246.50

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Western blot analysis of extracts of Rat brain, using EGFR antibody (STJ113559) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-EGFR antibody (1100-1200) (STJ113559)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti EGFR Goat Polyclonal antibody immunohistochemistry human STJ140129

Anti-EGFR antibody (1162aa C-Term) (STJ140129)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-P/IHC-F

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/Avian/Donkey/Feline/Goat/Guinea Pig/Hamster/Horse/Porcine/Rabbit/Sheep/Simian

£135.50 - £440.50

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Human appendix tissue was stained with Anti-EGFR (PTR2121) Antibody

Anti-EGFR antibody (300-400) [PTR2121] (STJA0006243)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£49.00 - £261.00

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Western blot analysis of lysates from wild type (WT) and EGFR knockdown (KD) HeLa cells, using [KD Validated] EGFR Rabbit monoclonal antibody (STJ11105117) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-EGFR antibody (320-420 aa) [S5117RM] (STJ11105117)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Anti-EGFR antibody IHC human lung squamous cell carcinoma STJ180567

Anti-EGFR antibody (344-492aa) [ZR16] (STJ180567)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£155.50 - £604.50

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Immunofluorescence of HaCaT cells stained with anti-human EGFR antibody showing nucleus actin and EGFR localization

Anti-EGFR antibody (424-605aa) [AT2H8] (STJA0041614)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF

Reactivity :

Human

£219.50 - £332.50

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ICC IF of EGFR in HeLa cells stained with Human EGFR antibody STJA0041914

Anti-EGFR antibody (424-605aa) [AT2H8] (STJA0041914)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF

Reactivity :

Human

£238.50 - £361.50

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ICC IF of EGFR in HeLa cells stained with STJA0041600 antibody Alexa Fluor 488 secondary green and DAPI nuclear stain blue

Anti-EGFR antibody (424-605aa) [AT6E3] (STJA0041600)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF

Reactivity :

Human

£219.50 - £332.50

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ICC IF of EGFR in HeLa cells stained with STJA0041921 antibody Alexa Fluor 488 secondary DAPI nucleus

Anti-EGFR antibody (424-605aa) [AT6E3] (STJA0041921)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF

Reactivity :

Human

£238.50 - £361.50

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Western blot analysis of various cells using EGFR Polyclonal Antibody diluted at 1：1000

Anti-EGFR antibody (661-710 aa) (STJ92850)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-EGFR antibody (800-900) [S9MR] (STJ11102229)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Immunohistochemistry analysis of paraffin-embedded Carcinoma of Human lung tissue using EGFR antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-EGFR antibody (900-1210 aa) [1B10] (STJA0013541)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

£224.50 - £308.50

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Immunofluorescence analysis of human-liver tissue. 1, EGFR Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-EGFR antibody (986-1035 aa) (STJ92862)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-EGFR antibody (N-Term) (STJA0005395)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£319.50

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Western blot A431 cells EGFR phosphorylation detection using antibody STJA0041200

Anti-EGFR antibody (N-Term) (STJA0041200)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

£474.50

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EGFR antibody western blot A549 cells STJA0033223

Anti-EGFR antibody [1A11-G9-1E9] (STJA0033223)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human

£133.50 - £281.50

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Anti-EGFR antibody [2+00E+09] (STJ16100028)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/IP/EM

Reactivity :

Human

£260.50

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EGFR antibody immunofluorescence HeLa cells STJA0032850

Anti-EGFR antibody [3A9-9D2-9F7] (STJA0032850)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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Western blot detection of EGFR in A549, MDA-MB-468 and COS7 cell lysates using EGFR mouse mAb (dilution 1:1000).Predicted band size:134 Kda.Observed band size:175KDa.

Anti-EGFR antibody [5E10-G10-E11] (STJ99229)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Human/Monkey

£149.00 - £360.00

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EGFR antibody western blot A549 MDA-MB-468 COS7 STJA0033224

Anti-EGFR antibody [5E10-G10-E11] (STJA0033224)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Monkey

£133.50 - £281.50

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EGFR 5E9 antibody western blot A431 HeLa STJA0032805

Anti-EGFR antibody [5E9-10B8-8B9] (STJA0032805)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human

£133.50 - £281.50

Choose Options