Anti-E2F6 antibody (1-281) (STJ116186)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal E2F6 (1-281) antibody for use in WB, IF, ICC, IP, ELISA and ChIP in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/IP/ELISA/ChIP |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | E2F6 |
| Gene ID | 1876 |
| Uniprot ID | E2F6_HUMAN |
| Immunogen Region | 1-281 |
| Immunogen Sequence | MSQQRPARKLPSLLLDPTEE TVRRRCRDPINVEGLLPSKI RINLEDNVQYVSMRKALKVK RPRFDVSLVYLTRKFMDLVR SAPGGILDLNKVATKLGVRK RRVYDITNVLDGIDLVEKKS KNHIRWIGSDLSNFGAVPQQ KKLQEELSDLSAMEDALDEL IKDCAQQLFELTDDKENERL AYVTYQDIHSIQAFHEQIVI AVKAPAETRLDVPAPREDSI TVHIRSTNGPIDVYLCEVE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-281 of human E2F6 (NP_937987.2). |
Additional Info
| Tissue Specificity | Expressed in all tissues examined. Highest levels in placenta, skeletal muscle, heart, ovary, kidney, small intestine and spleen. |
| Function | Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTCCGCGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. Represses expression of some meiosis-specific genes, including SLC25A31/ANT4. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase. |
| Protein Name | Transcription Factor E2f6E2f-6 |
| Database Links | Reactome: R-HSA-69205Reactome: R-HSA-8953750 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Transcription Factor E2f6 antibodyAnti-E2f-6 antibodyAnti-E2F6 antibody |
Information sourced from Uniprot.org