• Chromatin immunoprecipitation analysis of extracts of 293T cells, using E2F6 antibody (STJ116186) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Immunoprecipitation analysis of 200 Mu g extracts of MCF-7 cells using 3 Mu g E2F6 antibody (STJ116186). Western blot was performed from the immunoprecipitate using E2F6 antibody (STJ116186) at a dilution of 1:500.
  • Immunofluorescence analysis of U2OS cells using E2F6 Rabbit polyclonal antibody (STJ116186). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Mouse lung using E2F6 Rabbit polyclonal antibody (STJ116186) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:180s.

Anti-E2F6 antibody (1-281) (STJ116186)

SKU:
STJ116186

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-E2F6 (1-281) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
IP 1:20-1:50
ChIP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: E2F6
Gene ID: 1876
Uniprot ID: E2F6_HUMAN
Immunogen Region: 1-281
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-281 of human E2F6 (NP_937987.2).
Immunogen Sequence: MSQQRPARKLPSLLLDPTEE TVRRRCRDPINVEGLLPSKI RINLEDNVQYVSMRKALKVK RPRFDVSLVYLTRKFMDLVR SAPGGILDLNKVATKLGVRK RRVYDITNVLDGIDLVEKKS KNHIRWIGSDLSNFGAVPQQ KKLQEELSDLSAMEDALDEL IKDCAQQLFELTDDKENERL AYVTYQDIHSIQAFHEQIVI AVKAPAETRLDVPAPREDSI TVHIRSTNGPIDVYLCEVE
Tissue Specificity Expressed in all tissues examined. Highest levels in placenta, skeletal muscle, heart, ovary, kidney, small intestine and spleen.
Function Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTCCGCGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. Represses expression of some meiosis-specific genes, including SLC25A31/ANT4. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase.
Protein Name Transcription Factor E2f6
E2f-6
Database Links Reactome: R-HSA-69205
Reactome: R-HSA-8953750
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Factor E2f6 antibody
Anti-E2f-6 antibody
Anti-E2F6 antibody

Information sourced from Uniprot.org

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