• Western blot analysis of various lysates, using EED rabbit polyclonal antibody (STJ27324) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunofluorescence analysis of A-549 cells using EED rabbit polyclonal antibody (STJ27324) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using EED rabbit polyclonal antibody (STJ27324) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using EED rabbit polyclonal antibody (STJ27324) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g EED antibody (STJ27324). Western blot was performed from the immunoprecipitate using EED antibody (STJ27324) at a dilution of 1:1000.

Anti-EED antibody (1-400) (STJ27324)

SKU:
STJ27324

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Host: Rabbit
Applications: WB/IP/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EED (1-400) is suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EED
Gene ID: 8726
Uniprot ID: EED_HUMAN
Immunogen Region: 1-400
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-400 of human EED (NP_694536.1).
Immunogen Sequence: MSEREVSTAPAGTDMPAAKK QKLSSDENSNPDLSGDENDD AVSIESGTNTERPDTPTNTP NAPGRKSWGKGKWKSKKCKY SFKCVNSLKEDHNQPLFGVQ FNWHSKEGDPLVFATVGSNR VTLYECHSQGEIRLLQSYVD ADADENFYTCAWTYDSNTSH PLLAVAGSRGIIRIINPITM QCIKHYVGHGNAINELKFHP RDPNLLLSVSKDHALRLWNI QTDTLVAIFGGVEGHRDEV
Tissue Specificity Expressed in brain, colon, heart, kidney, liver, lung, muscle, ovary, peripheral blood leukocytes, pancreas, placenta, prostate, spleen, small intestine, testis, thymus and uterus. Appears to be overexpressed in breast and colon cancer.
Post Translational Modifications Methylated. Binding to histone H1 'Lys-26' promotes mono-, di-, and trimethylation of internal lysines.
Function Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
Protein Name Polycomb Protein Eed
Heed
Embryonic Ectoderm Development Protein
Wd Protein Associating With Integrin Cytoplasmic Tails 1
Wait-1
Database Links Reactome: R-HSA-212300
Reactome: R-HSA-2559580
Reactome: R-HSA-3214841
Reactome: R-HSA-5617472
Reactome: R-HSA-8943724
Reactome: R-HSA-8953750
Reactome: R-HSA-9609690
Reactome: R-HSA-9710421
Cellular Localisation Nucleus
Chromosome
Transiently Colocalizes With Xist At Inactive X Chromosomes
Alternative Antibody Names Anti-Polycomb Protein Eed antibody
Anti-Heed antibody
Anti-Embryonic Ectoderm Development Protein antibody
Anti-Wd Protein Associating With Integrin Cytoplasmic Tails 1 antibody
Anti-Wait-1 antibody
Anti-EED antibody

Information sourced from Uniprot.org

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