Anti-E2F4 antibody (250-350) [S8MR] (STJ11103078)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal E2F4 (250-350) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S8MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | E2F4 |
| Gene ID | 1874 |
| Uniprot ID | E2F4_HUMAN |
| Immunogen Region | 250-350 |
| Immunogen Sequence | TAVPGSAEVQGMAGPAAEIT VSGGPGTDSKDSGELSSLPL GPTTLDTRPLQSSALLDSSS SSSSSSSSSSNSNSSSSSGP NPSTSFEPIKADPTGVLELP K |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 250-350 of human E2F4 (Q16254). |
Additional Info
| Tissue Specificity | Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. |
| Post Translational Modifications | Differentially phosphorylated in vivo. |
| Function | Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTCCGCGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis. |
| Protein Name | Transcription Factor E2f4E2f-4 |
| Database Links | Reactome: R-HSA-1362277Reactome: R-HSA-1362300Reactome: R-HSA-1538133Reactome: R-HSA-2173796Reactome: R-HSA-6804114Reactome: R-HSA-69202Reactome: R-HSA-69205Reactome: R-HSA-69231Reactome: R-HSA-69656 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Transcription Factor E2f4 antibodyAnti-E2f-4 antibodyAnti-E2F4 antibody |
Information sourced from Uniprot.org