Gene Expression And Transcription | Research Area | St John's Laboratory

Western blot analysis of 293T lysis using KRAS antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000

Anti-KRAS antibody (150-189 aa) (STJ97704)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-KRAS antibody (30-188 aa) (STJ11106798)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of extracts from normal (control) and KRAS knockout (KO) 293T cells, using KRAS antibody (STJ114577) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-KRAS antibody (30-189) (STJ114577)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunofluorescence analysis of NIH/3T3 cells using KRAS Rabbit polyclonal antibody (STJ24344) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-KRAS antibody (30-189) (STJ24344)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from HEK293 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-KRBA1 antibody (738-788 aa) (STJ195786)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using KRBOX4 antibody (STJ28554) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-KRBOX4 antibody (1-98) (STJ28554)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of various lysates using KRBOX4 Rabbit polyclonal antibody (STJ116725) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-KRBOX4 antibody (C-Term) (STJ116725)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using L3MBTL1 antibody (STJ29170). Green:GFP-RNF168 fusion protein expression for DNA damage marker. Blue:DAPI for nuclear staining. RNF168 (GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.

Anti-L3MBTL1 antibody (533-772) (STJ29170)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-L3MBTL1 antibody (550-600 aa) (STJ13101326)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Western blot analysis of extracts of various cell lines, using L3MBTL2 antibody (STJ112369) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-L3MBTL2 antibody (486-705) (STJ112369)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-Lactic acid-Histone H4-K16 antibody (STJ11106549)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Anti-Lactic acid-Histone H4-K8 antibody (STJ11106550)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Western blot analysis of lysates from wild type (WT) and LAMTOR1 knockout (KO) HeLa cells, using [KO Validated] LAMTOR1 Rabbit polyclonal antibody (STJ11100859) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-LAMTOR1 antibody (1-161) (STJ11100859)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Confocal immunofluorescence analysis of U2OS cells using LAMTOR1 Rabbit polyclonal antibody (STJ11104413) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-LAMTOR1 antibody (1-161) (STJ11104413)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunofluorescence analysis of U-2 OS cells using LAMTOR1 Rabbit polyclonal antibody (STJ113224) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-LAMTOR1 antibody (1-161) (STJ113224)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-LAMTOR2 antibody (10-90 aa) (STJ190366)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-LAMTOR3 antibody (1-124 aa) (STJ11107462)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-LAMTOR3 antibody (30-110 aa) (STJ191720)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-LAMTOR4 antibody (8-58 aa) (STJ196554)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of NIH/3T3 cells using LAMTOR5 Rabbit polyclonal antibody (STJ29891) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-LAMTOR5 antibody (1-173) (STJ29891)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-LAMTOR5 antibody (1-50 aa) (STJ190865)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of U-2 OS cells using LBR Polyclonal Antibody (STJ27421) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-LBR antibody (1-211) (STJ27421)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-LBR antibody (411-461) (STJA0006601)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of LDB1 in paraffin-embedded human colon using LDB1 Rabbit polyclonal antibody (STJ119652) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-LDB1 antibody (1-50) (STJ119652)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-LDB1 antibody (STJA0008579)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Human tonsil stained with anti-LEF1 using peroxidase conjugate and DAB chromogen. Note nuclear staining of subset of B cells.

Anti-LEF1 antibody (100-200aa) [ZR336] (STJ180463)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£190.50 - £739.50

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Western blot analysis of extracts of various cell lines, using LEF1 antibody (STJ114908) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-LEF1 antibody (20-132) (STJ114908)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using LEF1 Rabbit monoclonal antibody (STJ11102088) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-LEF1 antibody (50-150) [S8MR] (STJ11102088)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Anti-LEO1 antibody (159-209 aa) (STJ196067)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using LEO1 antibody (STJ11103634) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-LEO1 antibody (340-570) (STJ11103634)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot with Anti-LGALS3 Antibody (STJ500375) with peptide on at 1:500 dilution in buffer.

Anti-LGALS3 antibody (1-100) (STJ500375)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Immunoprecipitation analysis of 300 Mu g extracts of HT-29 cells using 3 Mu g Galectin 3/LGALS3 antibody (STJ11101290). Western blot was performed from the immunoprecipitate using Galectin 3/LGALS3 antibody (STJ11101290) at a dilution of 1:1000.

Anti-LGALS3 antibody (1-100) [S0MR] (STJ11101290)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-LGALS3 antibody (1-100) {Biotin} (STJ500377)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-LGALS3 antibody (1-100) {FITC} (STJ500378)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Immunofluorescence analysis of U-2 OS cells using [KO Validated] Galectin 3/LGALS3 Rabbit polyclonal antibody (STJ115467) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-LGALS3 antibody (1-250) (STJ115467)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of extracts of various cell lines, using Galectin 3/LGALS3 antibody (STJ24401) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 5s.

Anti-LGALS3 antibody (1-250) (STJ24401)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of Hela cell. 1, CBP 35 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-LGALS3 antibody (141-190 aa) (STJ92060)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry of mouse Colon with Anti-LGALS3 primary antibody (STJ500376) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain.

Anti-LGALS3 antibody (150-250) (STJ500376)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-LGALS3 antibody (150-250) {Biotin} (STJ500379)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-LGALS3 antibody (150-250) {FITC} (STJ500380)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Various whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-Galectin-3 antibody. The HRP-conjugated anti- mouse IgG antibody was used to detect the antibody. Predicted band size: 26 (30) kDaObserved band size: 33 kDa

Anti-LGALS3 antibody (200-300 aa) [ABT-GAL3] (STJ197048)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human

£174.00 - £252.00

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Western blot analysis of 1) MCF7, 2) 3T3 with Galectin-3 Mouse mAb diluted at 1:2, 000.

Anti-LGALS3 antibody [8D7] (STJ97550)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from mouse skeletal muscle, using LIFR Rabbit polyclonal antibody (STJ114935) at 1:3000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 30s.

Anti-LIFR antibody (500-700) (STJ114935)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Western blot analysis of 293 cells using LIFR Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-LIFR antibody (731-780 aa) (STJ97299)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Formalin-fixed, paraffin-embedded human lymph node stained with anti-LMO2 antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of germinal center cells

Anti-LMO-2 antibody [ZR87] (STJ180579)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£211.50 - £938.50

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Western blot analysis of extracts of U-251MG cells, using LMO1 antibody (STJ29699) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-LMO1 antibody (1-156) (STJ29699)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of lysates from TF-1 cells using LMO2 Rabbit polyclonal antibody (STJ11105542) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:30s.

Anti-LMO2 antibody (1-100) (STJ11105542)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using LMO2 antibody (STJ11103611) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-LMO2 antibody (1-100) [S1MR] (STJ11103611)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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