DNA Repair | Research Area | St John's Laboratory

Anti-Phospho-H2BC3-Ser-36 antibody (STJA0005438)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£365.50

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Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-H2BC9-Ser14 antibody (STJ194711)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-Phospho-H4C1-Tyr-72 antibody (STJA0005439)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£365.50

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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Histone H2AX-S139 antibody (STJ27513) at 1:500 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-Histone H2A-S139 antibody (STJ27513)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Histone H2AX-S139 antibody (STJ11102561) at 1:1000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-Histone H2A-S139 antibody [S1MR] (STJ11102561)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-Phospho-Histone H2A-S2 antibody (STJ110882)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Western blot analysis of extracts of NIH/3T3 cells, using phospho-STK4-T387 polyclonal antibody (STJ11101036) at 1:1000 dilution or Histone H4 rabbit polyclonal antibody (A1131). NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour or Nocodazole (50 ng/mL) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-Histone H4-S1 antibody (STJ11101036)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Western blot analysis of extracts from Hela cells, untreated (-) or treated, 1:5000. Secondary antibody (STJ99666) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-Histone H4-Ser1 antibody (STJ197451)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of HUVEC cells, using Histone H4 (Phospho-Ser47) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-Histone H4-Ser47 antibody (13-62 aa) (STJ91343)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/IHC/WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-Histone H4-Thr80 antibody (STJ197450)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-HMGN1-Ser21 antibody (10-59 aa) (STJ91313)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of Jurkat cells using Phospho-TIP60 (S86) Polyclonal Antibody

Anti-Phospho-KAT5-Ser86 antibody (52-101 aa) (STJ90502)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse

£48.00 - £252.00

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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Tip60 (Phospho-Ser90) Antibody

Anti-Phospho-KAT5-Ser90 antibody (56-105 aa) (STJ91271)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-LIG4-Thr650 antibody (616-665 aa) (STJ91183)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-Phospho-MAPK8-Thr-183/Tyr-185 antibody [M268] (STJA0005456)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£365.50

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Immunohistochemistry analysis of paraffin-embedded human brain, using MDC1 (Phospho-Ser513) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MDC1-Ser513 antibody (479-528 aa) (STJ91260)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of 3T3 cells using Phospho-MRE11 (S264) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-MRE11-Ser264 antibody (230-279 aa) (STJ90554)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from HeLa cells using Phospho-MRE11 (Ser676) Rabbit polyclonal antibody (STJ11105909) at 1:800 dilution. HeLa cells were treated by Calyculin A (200 nM) at 37 °C for 18 hours after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-MRE11-Ser676 antibody (STJ11105909)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£129.50 - £642.50

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-MRE11-Ser676 antibody (STJ196358)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from HUVEC cells treated with Forskolin 40nM 30', using Nibrin (Phospho-Ser278) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-NBN-Ser278 antibody (251-300 aa) (STJ90727)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from Jurkat cells, using p95/NBS1 (Phospho-Ser343) Antibody. The lane on the left is blocked with the phospho peptide.

Anti-Phospho-NBN-Ser343 antibody (310-359 aa) (STJ90354)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£48.00 - £252.00

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Anti-Phospho-NBN-Ser343 antibody (STJ196375)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-Phospho-NBS1/NBN-S343 antibody (STJ11107709)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Anti-Phospho-p53-S20 antibody (STJ11107670)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of extracts of C6 cells, using Phospho-p53-T81 antibody (STJ11103770) at 1:500 dilution. C6 cells were treated by nocodazole (50 ng/ml) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-p53-T81 antibody (STJ11103770)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£129.50 - £642.50

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-PNKP-Ser114/Thr118 antibody (STJ196388)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of 293T cells, using Phospho-POLR2A-S2 antibody (STJ116378) at 1:1000 dilution. 293T cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour or treated by Doxorubicin (0. 5uM) for 24 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-POLR2A-S2 antibody (STJ116378)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of extracts of various cell lines, using Phospho-POLR2A CTD-S2 rabbit monoclonal antibody (STJ11102585) at 1:1000 dilution. MCF7 cells and HeLa cells and C2C12 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-POLR2A-S2 antibody [S5MR] (STJ11102585)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA/ChIP/ChIP-seq/CUT&Tag

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of HeLa, using Phospho-POLR2A CTD-S2/S5 antibody (STJ11105825) at 1:1000 dilution. Hela cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 5s.

Anti-Phospho-POLR2A-S2/S5 antibody (STJ11105825)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of extracts of various cell lines, using Phospho-POLR2A-S5 antibody (STJ117926) at 1:1000 dilution. MCF7 and C2C12 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-POLR2A-S5 antibody (STJ117926)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of extracts of various cell lines, using Phospho-POLR2A CTD-S5 rabbit monoclonal antibody (STJ11102586) at 1:1000 dilution. MCF7 cells were treated by Doxorubicin (0. 5 uM) at 37 °C for 24 hours or treated by nocodazole (50 ng/mL) at 37 °C for 20 hours or treated by CIP (20uL/400ul) at 37 °C for 1 hour. C2C12 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-POLR2A-S5 antibody [S6MR] (STJ11102586)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of extracts of MCF7 cells, using Phospho-POLR2A CTD-S7 antibody (STJ11103724) at 1:1000 dilution. MCF7 cells were treated by Doxorubicin (0. 5 uM) at 37 °C for 24 hours. MCF7 cells were treated by nocodazole (50 ng/ml) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-POLR2A-S7 antibody (STJ11103724)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA/ChIP

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of VEC using p-Rpb1 (S1619) antibody. Antibody was diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-POLR2A-Ser1619 antibody (1585-1634 aa) (STJ91031)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Anti-Phospho-POLR2A-Ser2 antibody (STJ196405)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-POLR2A-Ser5 antibody (STJ196406)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-POLR2A-Ser7 antibody (STJ196407)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Chromatin immunoprecipitation analysis of extracts of 293T; cells, using Phospho-POLR2A CTD-T4 antibody (STJ11104227) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Phospho-POLR2A-T4 antibody (STJ11104227)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA/ChIP

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates, using Phospho-POLR2A-T4 monoclonal antibody antibody (STJ11105859) at 1:6000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-POLR2A-T4 antibody [S5859RM] (STJ11105859)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-Phospho-POLR2A-Thr4 antibody (STJ196408)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-POLR2A-Thr4 antibody (STJ196409)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of HeLa cells, using Phospho-DNA-PKcs-S2056 antibody (STJ113503). Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.

Anti-Phospho-PRKDC-S2056 antibody (STJ113503)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

£129.50 - £642.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using DNA-PK (Phospho-Ser2056) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PRKDC-Ser2056 antibody (2022-2071 aa) (STJ91147)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-Phospho-PRKDC-Ser2612 antibody (2578-2627 aa) (STJ91146)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-Phospho-PRKDC-Thr2609 antibody (STJA0009815)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of HUVEC cells treated with serum 20% 30', using DNA-PK (Phospho-Thr2638) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PRKDC-Thr2638 antibody (2604-2653 aa) (STJ91334)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using DNA-PK (Phospho-Thr2647) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PRKDC-Thr2647 antibody (2613-2662 aa) (STJ91305)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-RAD17-Ser635 antibody (STJ196393)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-Rad17 (S645) Polyclonal Antibody

Anti-Phospho-RAD17-Ser645 antibody (621-670 aa) (STJ90451)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse

£48.00 - £252.00

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