• Immunohistochemistry analysis of MUS81 in paraffin-embedded mouse brain using MUS81 Rabbit polyclonal antibody (STJ28901) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MUS81 in paraffin-embedded rat brain using MUS81 Rabbit polyclonal antibody (STJ28901) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using MUS81 Rabbit polyclonal antibody (STJ28901) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MUS81 antibody (1-350) (STJ28901)

SKU:
STJ28901

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MUS81 (1-350) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MUS81
Gene ID: 80198
Uniprot ID: MUS81_HUMAN
Immunogen Region: 1-350
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human MUS81 (NP_079404.3).
Immunogen Sequence: MAAPVRLGRKRPLPACPNPL FVRWLTEWRDEATRSRRRTR FVFQKALRSLRRYPLPLRSG KEAKILQHFGDGLCRMLDER LQRHRTSGGDHAPDSPSGEN SPAPQGRLAEVQDSSMPVPA QPKAGGSGSYWPARHSGARV ILLVLYREHLNPNGHHFLTK EELLQRCAQKSPRVAPGSAR PWPALRSLLHRNLVLRTHQP ARYSLTPEGLELAQKLAESE GLSLLNVGIGPKEPPGEET
Tissue Specificity Widely expressed.
Function Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. Plays an essential role in mitosis for the processing of stalled or collapsed replication forks.
Protein Name Crossover Junction Endonuclease Mus81
Database Links Reactome: R-HSA-5693568
Reactome: R-HSA-6783310
Cellular Localisation Nucleus
Nucleolus
Recruited To Foci Of Dna Damage In S-Phase Cells
Alternative Antibody Names Anti-Crossover Junction Endonuclease Mus81 antibody
Anti-MUS81 antibody

Information sourced from Uniprot.org

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