• Western blot analysis of extracts of various cell lines, using NBS1/NBS1/NBN antibody (STJ110016) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.
  • Immunofluorescence analysis of U2OS cells using NBS1/NBS1/NBN antibody (STJ110016) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-NBN antibody (650-750) (STJ110016)

SKU:
STJ110016

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NBN (650-750) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NBN
Gene ID: 4683
Uniprot ID: NBN_HUMAN
Immunogen Region: 650-750
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 650-750 of human NBS1/NBS1/NBN (NP_002476.2).
Immunogen Sequence: LPKKLLLTEFRSLVIKNSTS RNPSGINDDYGQLKNFKKFK KVTYPGAGKLPHIIGGSDLI AHHARKNTELEEWLRQEMEV QNQHAKEESLADDLFRYNPY L
Tissue Specificity Ubiquitous. Expressed at high levels in testis.
Post Translational Modifications Phosphorylated by ATM in response of ionizing radiation, and such phosphorylation is responsible intra-S phase checkpoint control and telomere maintenance.
Function Component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex.
Protein Name Nibrin
Cell Cycle Regulatory Protein P95
Nijmegen Breakage Syndrome Protein 1
Database Links Reactome: R-HSA-2559586
Reactome: R-HSA-5685938
Reactome: R-HSA-5685939
Reactome: R-HSA-5685942
Reactome: R-HSA-5693548
Reactome: R-HSA-5693554
Reactome: R-HSA-5693565
Reactome: R-HSA-5693568
Reactome: R-HSA-5693571
Reactome: R-HSA-5693579
Reactome: R-HSA-5693607
Reactome: R-HSA-5693616
Reactome: R-HSA-6804756
Reactome: R-HSA-69473
Reactome: R-HSA-912446
Reactome: R-HSA-9701192
Reactome: R-HSA-9704331
Reactome: R-HSA-9704646
Reactome: R-HSA-9709570
Reactome: R-HSA-9709603
Cellular Localisation Nucleus
Pml Body
Chromosome
Telomere
Localizes To Discrete Nuclear Foci After Treatment With Genotoxic Agents
Acetylation Of 'Lys-5' Of Histone H2ax (H2axk5ac) Promotes Nbn/Nbs1 Assembly At The Sites Of Dna Damage
Alternative Antibody Names Anti-Nibrin antibody
Anti-Cell Cycle Regulatory Protein P95 antibody
Anti-Nijmegen Breakage Syndrome Protein 1 antibody
Anti-NBN antibody
Anti-NBS antibody
Anti-NBS1 antibody
Anti-P95 antibody

Information sourced from Uniprot.org

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