Developmental Biology | Research Area | St John's Laboratory

Immunohistochemical analysis of paraffin-embedded human brain tumor. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-MAPK3 antibody (STJ197394)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using ERK 1 monoclonal antibody against HeLa (1) , Jurkat (2) , RAW264.7 (3) , HEK293 (4) , K562 (5) , NIH/3T3 (6) , Cos7 (7) and PC-12 (8) cell lysate.

Anti-MAPK3 antibody [1E5] (STJ98045)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£144.00 - £348.00

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Immunofluorescence analysis of rat-spleen tissue. 1, ERK1 Mouse monoclonal antibody (5E9) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-MAPK3 antibody [5E9] (STJ97780)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/WB/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of MCF-7 cells using ERK5 antibody (STJ24489). Blue: DAPI for nuclear staining.

Anti-MAPK7 antibody (1-815) (STJ24489)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-MAPK7 antibody (700-750) (STJ501711)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-MAPK7 antibody (700-750) {Biotin} (STJ501712)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-MAPK7 antibody (700-750) {FITC} (STJ501713)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western blot analysis of extracts of various cell lines, using ERK5 Rabbit monoclonal antibody (STJ11101971) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-MAPK7 antibody (717-816) [S1MR] (STJ11101971)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of 1) Hela, 2) 293T, 3) Mouse Skeletal Muscle, 4) Rat Kidney, 5) Rat Skeletal Muscle using ERK 5 Polyclonal Antibody. Secondary antibody (STJ99666) was diluted at 1:20000

Anti-MAPK7 antibody (STJ197395)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using MAPK8 Rabbit polyclonal antibody (STJ11105575) at 1:400 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 90s.

Anti-MAPK8 antibody (1-100) (STJ11105575)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of HeLa cells using JNK1 antibody (STJ24490) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-MAPK8 antibody (245-345) (STJ24490)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using JNK1 Rabbit polyclonal antibody (STJ24491) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MAPK8 antibody (245-345) (STJ24491)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of JNK1 in C6, 3T3, Hela lysates using JNK1 antibody.

Anti-MAPK8 antibody (325-343 aa) [R08-7H1] (STJA0011517)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£246.50

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Immunohistochemistry Colon with Anti-MAPK8 Antibody (STJ501714) at 1:50 dilution in Buffer. DAB (brown) staining and Haematoxylin QS (blue) counterstain. 5X magnification on Leica DM4000. FFPE section.

Anti-MAPK8 antibody (370-420) (STJ501714)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£427.50

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Anti-MAPK8 antibody (370-420) {Biotin} (STJ501715)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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Anti-MAPK8 antibody (370-420) {FITC} (STJ501716)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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Anti-MAPK8 antibody (C-Term) [M267] (STJA0005455)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IHC

Reactivity :

Human/Mouse/Rat

£390.50

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Western blot detection of JNK1 in C6 and 3T3 cell lysates using JNK1 mouse mAb (1:1000 diluted).Predicted band size:46, 54KDa.Observed band size:46KDa.

Anti-MAPK8 antibody [1A4-C5-F11] (STJ99225)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human/Mouse/Rat

£144.00 - £348.00

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Anti-Matrix Metalloproteinase-2 antibody [4D3] (STJ16100697)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P/WB

Reactivity :

Human

£771.50

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Anti-MBP antibody (100-140 aa) (STJ13101368)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

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Human cerebrum tissue was stained with Anti-Myelin Basic Protein (MBP) (ABT-MBP) Antibody

Anti-MBP antibody (150-250 aa) [ABT-MBP] (STJ196930)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Confocal imaging of paraffin-embedded Mouse brain using Myelin Basic Protein Rabbit monoclonal antibody (STJ11101285, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01 M citRate buffer (pH 6. 0) prior to IF staining.

Anti-MBP antibody (161-304) [S5MR] (STJ11101285)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£146.50 - £642.50

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-MBP antibody (180-260 aa) (STJ190136)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-MBP antibody (2-40 aa) (STJ13101425)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Western blot analysis of extracts of various cell lines, using Myelin Basic Protein antibody (STJ24515) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MBP antibody (80-165) (STJ24515)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-MBP antibody (N-Term) (STJA0005812)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£455.50

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Anti-MBP antibody (STJ13100186)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£331.50

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Anti-MECOM antibody (STJA0007991)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£48.00 - £252.00

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Western blot analysis of extracts of MCF7 cells, using MED1 antibody (STJ24540) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-MED1 antibody (1-300) (STJ24540)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of various cells using TRAP220 Polyclonal Antibody. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-MED1 antibody (1423-1472 aa) (STJ96090)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using TRAP220 Polyclonal Antibody diluted at 1：500. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-MED1 antibody (641-690 aa) (STJ96089)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-MED1 (PTR1203) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: DaudiPredicted band size: 220kDaObserved band size: 220kDa

Anti-MED1 antibody (650-750) [PTR1203] (STJA0006273)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£48.00 - £252.00

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-MED10 antibody (40-120 aa) (STJ190943)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-MED11 antibody (30-110 aa) (STJ190944)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-MED12 antibody (1800-1900 aa) (STJ11106467)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using MED12 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MED12 antibody (611-660 aa) (STJ96091)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-MED13 antibody (300-400) (STJ11101486)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using MED13 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MED13 antibody (681-730 aa) (STJ96092)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MED13L Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MED13L antibody (449-498 aa) (STJ96093)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using MED14 antibody (STJ112484) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-MED14 antibody (470-650) (STJ112484)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of COS7 cells, using MED14 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MED14 antibody (701-750 aa) (STJ92486)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of C6 cells using MED15 Rabbit polyclonal antibody (STJ11100195) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MED15 antibody (1-100) (STJ11100195)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-MED15 antibody (510-590 aa) (STJ190945)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-MED16 antibody (326-376 aa) (STJ197954)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using CRSP77 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-MED17 antibody (141-190 aa) (STJ92489)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using MED17 antibody (STJ116326) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-MED17 antibody (250-460) (STJ116326)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using MED18 Rabbit polyclonal antibody (STJ116798) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MED18 antibody (1-208) (STJ116798)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of various cells using Med18 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-MED18 antibody (70-119 aa) (STJ94061)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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