• Immunofluorescence analysis of U-2 OS cells using DAG1 Rabbit polyclonal antibody (STJ11104311) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using DAG1 Rabbit polyclonal antibody (STJ11104311) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of H9C2 cells using DAG1 Rabbit polyclonal antibody (STJ11104311) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from SK-BR-3 cells, using DAG1 Rabbit polyclonal antibody (STJ11104311) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-DAG1 antibody (654-749) (STJ11104311)

SKU:
STJ11104311

Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Dystroglycan 1 (654-749) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DAG1
Gene ID: 1605
Uniprot ID: DAG1_HUMAN
Immunogen Region: 654-749
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 654-749 of human DAG1 (NP_004384.4).
Immunogen Sequence: SIVVEWTNNTLPLEPCPKEQ IAGLSRRIAEDDGKPRPAFS NALEPDFKATSITVTGSGSC RHLQFIPVVPPRRVPSEAPP TEVPDRDPEKSSEDDV
Tissue Specificity Expressed in a variety of fetal and adult tissues. In epidermal tissue, located to the basement membrane. Also expressed in keratinocytes and fibroblasts.
Post Translational Modifications Alpha-dystroglycan: O-glycosylated. POMGNT1 catalyzes the initial addition of N-acetylglucosamine, giving rise to the GlcNAc(beta1-2)Man(alpha1-)O-Ser/Thr moiety and thus providing the necessary basis for the addition of further carbohydrate moieties. Heavily O-glycosylated comprising of up to two thirds of its mass and the carbohydrate composition differs depending on tissue type. Mucin-type O-glycosylation is important for ligand binding activity. O-mannosylation is found in high abundance in both brain and muscle where the most abundant glycan is Sia-alpha-2-3-Gal-beta-1-4-Glc-NAc-beta-1-2-Man. In muscle, glycosylation on Thr-317, Thr-319 and Thr-379 by a phosphorylated O-mannosyl glycan with the structure 2-(N-acetylamido)-2-deoxygalactosyl-beta-1,3-2-(N-acetylamido)-2-deoxyglucosyl-beta-1,4-6-phosphomannose is mediated by like-acetylglucosaminyltransferase (LARGE1) protein and is required for laminin binding. The O-glycosyl hexose on Thr-367, Thr-369, Thr-372, Thr-381 and Thr-388 is probably mannose. O-glycosylated in the N-terminal region with a core 1 or possibly core 8 glycan. The brain form displays a unique glycosylation pattern which is absent in other tissues.this form shows enhanced binding to laminin LAMA5 compared to the skeletal muscle form. Alpha-dystroglycan: (Microbial infection) O-mannosylation is required for binding lymphocytic choriomeningitis virus, Old World Lassa fever virus, and clade C New World arenaviruses. Beta-dystroglycan: N-glycosylated. Autolytic cleavage produces the alpha and beta subunits. In cutaneous cells, as well as in certain pathological conditions, shedding of beta-dystroglycan can occur releasing a peptide of about 30 kDa. SRC-mediated phosphorylation of the PPXY motif of the beta subunit recruits SH2 domain-containing proteins, but inhibits binding to WWW domain-containing proteins, DMD and UTRN. This phosphorylation also inhibits nuclear entry.
Function The dystroglycan complex is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. Alpha-dystroglycan: Extracellular peripheral glycoprotein that acts as a receptor for extracellular matrix proteins containing laminin-G domains. Receptor for laminin-2 (LAMA2) and agrin in peripheral nerve Schwann cells. Also acts as a receptor for laminin LAMA5. Beta-dystroglycan: Transmembrane protein that plays important roles in connecting the extracellular matrix to the cytoskeleton. Acts as a cell adhesion receptor in both muscle and non-muscle tissues. Receptor for both DMD and UTRN and, through these interactions, scaffolds axin to the cytoskeleton. Also functions in cell adhesion-mediated signaling and implicated in cell polarity. Alpha-dystroglycan: (Microbial infection) Acts as a receptor for lassa virus and lymphocytic choriomeningitis virus glycoprotein and class C new-world arenaviruses. Acts as a Schwann cell receptor for Mycobacterium leprae, the causative organism of leprosy, but only in the presence of the G-domain of LAMA2.
Protein Name Dystroglycan 1
Dystroglycan
Dystrophin-Associated Glycoprotein 1 Cleaved Into - Alpha-Dystroglycan
Alpha-Dg - Beta-Dystroglycan
Beta-Dg
Database Links Reactome: R-HSA-3000171
Reactome: R-HSA-3000178
Reactome: R-HSA-5083628
Reactome: R-HSA-5083629
Reactome: R-HSA-5083633
Reactome: R-HSA-5173105
Reactome: R-HSA-9010553
Reactome: R-HSA-9619665
Cellular Localisation Alpha-Dystroglycan: Secreted
Extracellular Space
Beta-Dystroglycan: Cell Membrane
Single-Pass Type I Membrane Protein
Cytoplasm
Cytoskeleton
Nucleus
Nucleoplasm
Cell Membrane
Sarcolemma
Postsynaptic Cell Membrane
The Monomeric Form Translocates To The Nucleus Via The Action Of Importins And Depends On Ran
Nuclear Transport Is Inhibited By Tyr-892 Phosphorylation
In Skeletal Muscle
This Phosphorylated Form Locates To A Vesicular Internal Membrane Compartment
In Muscle Cells
Sarcolemma Localization Requires The Presence Of Ank2
While Localization To Costameres Requires The Presence Of Ank3
Localizes To Neuromuscular Junctions (Nmjs) In The Presence Of Ank2
In Peripheral Nerves
Localizes To The Schwann Cell Membrane
Colocalizes With Erm Proteins In Schwann-Cell Microvilli
Alternative Antibody Names Anti-Dystroglycan 1 antibody
Anti-Dystroglycan antibody
Anti-Dystrophin-Associated Glycoprotein 1 Cleaved Into - Alpha-Dystroglycan antibody
Anti-Alpha-Dg - Beta-Dystroglycan antibody
Anti-Beta-Dg antibody
Anti-DAG1 antibody

Information sourced from Uniprot.org

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