Cell Cycle | Research Area | St John's Laboratory

Immunohistochemistry analysis of paraffin-embedded human kidney using Phospho-Akt-T308 Rabbit monoclonal antibody (STJ11105823) at dilution of 1:60 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-AKT1-T308 antibody [S5823RM] (STJ11105823)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Western blot analysis of lysates from RD cells, using Phospho-AKT1-T450 Rabbit pAb (STJ22008) at 1:600 dilution. RD cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-AKT1-T450 antibody (STJ22008)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.57 - $853.53

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Western blot analysis of lysates from C6 cells, using Phospho-AKT1-T450 Rabbit mAb (STJ11102572) at 1:1000 dilution. C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour or treated by PDGF (50 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-AKT1-T450 antibody [S2MR] (STJ11102572)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

$163.53 - $719.67

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Anti-Phospho-AKT1-Thr-34 antibody (STJA0005253)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rabbit/Rat

$504.39

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Western blot analysis of various cells using Phospho-Akt1 (T450) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Thr450 antibody (416-465 aa) (STJ90168)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Phospho-AKT1 Thr450 antibody western blot U2OS C6 STJA0035050

Anti-Phospho-AKT1-Thr450 antibody [R07-4A7] (STJA0035050)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Rat

$184.23 - $388.47

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Phospho-AKT1 antibody western blot 3T3 lysates STJA0035669

Anti-Phospho-AKT1-Thr450 antibody [R72-1U-9] (STJA0035669)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Western blot analysis of 3T3 cells using Phospho-Akt1 (T72) Polyclonal Antibody

Anti-Phospho-AKT1-Thr72 antibody (38-87 aa) (STJ90844)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of various cells using Phospho-Akt (Y326) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Tyr326 antibody (292-341 aa) (STJ90503)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of various cells using Phospho-Akt1 (Y474) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Tyr474 antibody (431-480 aa) (STJ90843)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$67.62 - $360.18

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Western blot analysis of lysates from 293F cells using Phospho-AKT2 specific-S474 Rabbit polyclonal antibody (STJ11105868) at 1:900 dilution. 293F cells, 293F cells transfected with AKT3 and 293F cells transfected with AKT2 were treated by Calyculin A (50 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-AKT2-S474 antibody (STJ11105868)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.57 - $853.53

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Anti-Phospho-AKT2-S474 antibody [SRM13696] (STJ11108426)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

$198.03 - $853.53

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Western blot analysis of various cells using Phospho-Akt2 (S474) Polyclonal Antibody diluted at 1：500

Anti-Phospho-AKT2-Ser474 antibody (432-481 aa) (STJ90169)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Phospho-Alpha 6-Tubulin-Ser-165 antibody (STJA0041427)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

$748.65

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Phospho-alpha Tubulin Tyr272 antibody western blot Jurkat STJA0035837

Anti-Phospho-alpha Tubulin-Tyr272 antibody [R06-9Z1] (STJA0035837)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

$184.23 - $388.47

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Immunohistochemistry analysis of paraffin-embedded human brain, using APC1 (Phospho-Ser355) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ANAPC1-Ser355 antibody (321-370 aa) (STJ91228)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using APC1 (Phospho-Ser688) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ANAPC1-Ser688 antibody (654-703 aa) (STJ91312)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of various lysates using (STJ22028) at 1:1000 dilution. 293T cells were treated by Doxorubicin (0. 5 uM) at 37 °C for 24 hours. 293T cells were treated by UV at room temperature for 15-30 minutes. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-ATM-S1981 antibody (STJ22028)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.57 - $853.53

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Confocal imaging of paraffin-embedded Human colon tissue (untreated) and Human colon tissue (treated with Lambda pp) using Phospho-ATM-S1981 Rabbit mAb (STJ11102610, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (STJS001166, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.

Anti-Phospho-ATM-S1981 antibody [S0MR] (STJ11102610)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

$163.53 - $719.67

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Anti-Phospho-ATM-Ser-1981 antibody [M366] (STJA0005274)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/ICC

Reactivity :

Human/Mouse/Rat

$504.39

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Western blot ATM phosphorylation in A431 cells with phosphatase treatment comparison STJA0041054

Anti-Phospho-ATM-Ser-1981 antibody [M366] (STJA0041054)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/IP/ICC

Reactivity :

Human/Mouse/Rat

$748.65

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Anti-Phospho-ATM-Ser-794 antibody (STJA0005273)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/FC

Reactivity :

Human/Mouse/Rat

$504.39

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ICC staining ATM phosphorylation in A431 cells using anti-ATM Ser-794 antibody STJA0041068

Anti-Phospho-ATM-Ser-794 antibody (STJA0041068)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC/FC

Reactivity :

Human/Mouse/Rat

$748.65

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Western Blot analysis of 1 K562 cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-ATM-Ser179 antibody (STJA0010053)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot analysis of K562 using p-Atm (S1981) antibody. Antibody was diluted at 1:500

Anti-Phospho-ATM-Ser1981 antibody (STJ97691)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Phospho-ATM-Ser1981 antibody [PT0902R] (STJA0039795)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Phospho-ATM Ser1981 antibody western blot HEK293 STJA0035784

Anti-Phospho-ATM-Ser1981 antibody [R01-5I8] (STJA0035784)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human

$184.23 - $388.47

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Western Blot analysis of mouse heart, Hela, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-ATM-Ser367 antibody (STJA0010035)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot analysis of lysates from 293T cells, using Phospho-ATR-S428 Rabbit pAb (STJ114848) at 1:400 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-ATR-S428 antibody (STJ114848)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of various lysates, using Phospho-ATR-S428 antibody (STJ11105842) at1:2000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-ATR-S428 antibody [S5842RM] (STJ11105842)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$163.53 - $719.67

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Anti-Phospho-ATR-S428-S428 antibody [SRM15157] (STJ11109887)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/IHC-P/ELISA

Reactivity :

Human

$163.53 - $719.67

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using ATR (Phospho-Ser428) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ATR-Ser428 antibody (394-443 aa) (STJ91234)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Anti-Phospho-ATR-Ser428 antibody [PT1243R] (STJA0040115)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Western blot analysis of lysates from HeLa cells, using Phospho-ATR-T1989 Rabbit pAb (STJ11103765) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-ATR-T1989 antibody (STJ11103765)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Anti-Phospho-ATR-Thr1989 antibody (STJ196248)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using ATRIP (Phospho-Ser224) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ATRIP-Ser224 antibody (190-239 aa) (STJ90973)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot ATRIP protein phosphorylation at Ser239 in mouse testicular lysate STJA0003585

Anti-Phospho-ATRIP-Ser239 antibody (STJA0003585)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse/Bovine/Human/Non-Human Primates/Sheep

$674.13

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Western blot analysis of NIH-3T3 cells using Phospho-ATRIP (S68) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-ATRIP-Ser68 antibody (34-83 aa) (STJ90496)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using AurA (Phospho-Ser342) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-AURKA-Ser342 antibody (311-360 aa) (STJ91174)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of various lysates, using Phospho-AURKA-T288 Rabbit pAb (STJ110859) at 1:1000 dilution. HeLa were treated by Hydroxyurea (4mM) or treated by Nocodazole (50ng/mL) for 20 hours. HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-AURKA-T288 antibody (STJ110859)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.57 - $853.53

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Western blot analysis of 293 cells using Phospho-ARK-1 (T288) Polyclonal Antibody

Anti-Phospho-AURKA-Thr288 antibody (256-305 aa) (STJ90805)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunohistochemistry analysis of paraffin-embedded human liver carcinoma, using AurB (Phospho-Thr232) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-AURKB-Thr232 antibody (198-247 aa) (STJ91089)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$67.62 - $360.18

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Western blot analysis of lysates from K562 cells, using AurB (Phospho-Tyr12) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-AURKB-Tyr12 antibody (1-50 aa) (STJ90920)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°C overnight STJ194723

Anti-Phospho-BABAM1-Ser29 antibody (STJ194723)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Anti-Phospho-Beta-Tubulin-Ser-172 antibody (STJA0041423)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

$748.65

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Immunohistochemistry analysis of paraffin-embedded human brain, using Survivin (Phospho-Thr117) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-BIRC5-Thr117 antibody (86-135 aa) (STJ91292)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from HT29 cells treated with EGF 100 μg/ml for 30 min, using Survivin (Phospho-Thr34) STJ90479

Anti-Phospho-BIRC5-Thr34 antibody (11-60 aa) (STJ90479)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunohistochemistry analysis of paraffin-embedded human heart, using Bloom Syndrome (Phospho-Thr99) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-BLM-Thr99 antibody (65-114 aa) (STJ91066)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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