• Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using Akt2 (Phospho-Ser474) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of 293 cells using Phospho-Akt2 (S474) Polyclonal Antibody diluted at 1:500
  • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Akt2 (Phospho-Ser474) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-Akt2 (S474) Polyclonal Antibody diluted at 1:500

Anti-Phospho-AKT2-Ser474 antibody (432-481 aa) (STJ90169)

SKU:
STJ90169

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-RAC-beta serine/threonine-protein kinase-Ser474 (432-481 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AKT2
Gene ID: 208
Uniprot ID: AKT2_HUMAN
Immunogen Region: 432-481 aa
Specificity: Phospho-Akt2 (S474) Polyclonal Antibody detects endogenous levels of Akt2 protein only when phosphorylated at S474.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Akt2 around the phosphorylation site of Ser474 at the amino acid range 432-481
Post Translational Modifications Phosphorylation on Thr-309 and Ser-474 is required for full activity. Ubiquitinated.undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT2 ubiquitination. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. O-GlcNAcylation at Thr-306 and Thr-313 inhibits activating phosphorylation at Thr-309 via disrupting the interaction between AKT and PDK1.
Function AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling. Phosphorylation of TBC1D4 triggers the binding of this effector to inhibitory 14-3-3 proteins, which is required for insulin-stimulated glucose transport. AKT regulates also the storage of glucose in the form of glycogen by phosphorylating GSK3A at 'Ser-21' and GSK3B at 'Ser-9', resulting in inhibition of its kinase activity. Phosphorylation of GSK3 isoforms by AKT is also thought to be one mechanism by which cell proliferation is driven. AKT regulates also cell survival via the phosphorylation of MAP3K5 (apoptosis signal-related kinase). Phosphorylation of 'Ser-83' decreases MAP3K5 kinase activity stimulated by oxidative stress and thereby prevents apoptosis. AKT mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. AKT is involved in the phosphorylation of members of the FOXO factors (Forkhead family of transcription factors), leading to binding of 14-3-3 proteins and cytoplasmic localization. In particular, FOXO1 is phosphorylated at 'Thr-24', 'Ser-256' and 'Ser-319'. FOXO3 and FOXO4 are phosphorylated on equivalent sites. AKT has an important role in the regulation of NF-kappa-B-dependent gene transcription and positively regulates the activity of CREB1 (cyclic AMP (cAMP)-response element binding protein). The phosphorylation of CREB1 induces the binding of accessory proteins that are necessary for the transcription of pro-survival genes such as BCL2 and MCL1. AKT phosphorylates 'Ser-454' on ATP citrate lyase (ACLY), thereby potentially regulating ACLY activity and fatty acid synthesis. Activates the 3B isoform of cyclic nucleotide phosphodiesterase (PDE3B) via phosphorylation of 'Ser-273', resulting in reduced cyclic AMP levels and inhibition of lipolysis. Phosphorylates PIKFYVE on 'Ser-318', which results in increased PI(3)P-5 activity. The Rho GTPase-activating protein DLC1 is another substrate and its phosphorylation is implicated in the regulation cell proliferation and cell growth. AKT plays a role as key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). AKT mediates the antiapoptotic effects of IGF-I. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. May be involved in the regulation of the placental development. Involved in the inhibition of ciliogenesis associated with RAB8-dependent cilia growth. One of the few specific substrates of AKT2 identified recently is PITX2. Phosphorylation of PITX2 impairs its association with the CCND1 mRNA-stabilizing complex thus shortening the half-life of CCND1. AKT2 seems also to be the principal isoform responsible of the regulation of glucose uptake. Phosphorylates C2CD5 on 'Ser-197' during insulin-stimulated adipocytes. AKT2 is also specifically involved in skeletal muscle differentiation, one of its substrates in this process being ANKRD2. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis. Phosphorylates CLK2 on 'Thr-343'.
Protein Name Rac-Beta Serine/Threonine-Protein Kinase
Protein Kinase Akt-2
Protein Kinase B Beta
Pkb Beta
Rac-Pk-Beta
Database Links Reactome: R-HSA-111447
Reactome: R-HSA-1257604
Reactome: R-HSA-1358803
Reactome: R-HSA-1445148
Reactome: R-HSA-165158
Reactome: R-HSA-165160
Reactome: R-HSA-165181
Reactome: R-HSA-198323
Reactome: R-HSA-198693
Reactome: R-HSA-199418
Reactome: R-HSA-211163
Reactome: R-HSA-3769402
Reactome: R-HSA-389357
Reactome: R-HSA-389513
Reactome: R-HSA-392451
Reactome: R-HSA-5218920
Reactome: R-HSA-5628897
Reactome: R-HSA-5674400
Reactome: R-HSA-6804757
Reactome: R-HSA-6804758
Reactome: R-HSA-6804759
Reactome: R-HSA-69202
Reactome: R-HSA-69656
Reactome: R-HSA-8876198
Reactome: R-HSA-8941332
Reactome: R-HSA-8948751
Reactome: R-HSA-9607240
Reactome: R-HSA-9614399
Reactome: R-HSA-9634638
Reactome: R-HSA-9755511
Reactome: R-HSA-9755779
Cellular Localisation Cytoplasm
Nucleus
Cell Membrane
Peripheral Membrane Protein
Early Endosome
Localizes Within Both Nucleus And Cytoplasm Of Proliferative Primary Myoblasts And Mostly Within The Nucleus Of Differentiated Primary Myoblasts
By Virtue Of The N-Terminal Ph Domain
Is Recruited To Sites Of The Plasma Membrane Containing Increased Pi(3
4
5)P3 Or Pi(3
4)P2
Cell Membrane Targeting Is Also Facilitared By Interaction With Clip3
Colocalizes With Wdfy2 In Early Endosomes
Alternative Antibody Names Anti-Rac-Beta Serine/Threonine-Protein Kinase antibody
Anti-Protein Kinase Akt-2 antibody
Anti-Protein Kinase B Beta antibody
Anti-Pkb Beta antibody
Anti-Rac-Pk-Beta antibody
Anti-AKT2 antibody

Information sourced from Uniprot.org

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