Polyclonal Antibody | Primary Antibodies | St John's Laboratory

Western blot analysis of various lysates using ALOXE3 Rabbit pAb (STJ110544) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-ALOXE3 antibody (1-280) (STJ110544)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-ALOXE3 antibody (467-517) (STJA0006307)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Anti-Alpaca-IgG antibody (STJA0010224)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Alpaca

$64.80 - $340.20

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Anti-ALPG antibody (10-90 aa) (STJ190382)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Anti-Alpha Fetoprotein antibody (50-100) (STJ501651)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Mouse

$577.13

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Anti-Alpha Fetoprotein antibody (50-100) {Biotin} (STJ501652)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Mouse

$713.48

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Anti-Alpha Fetoprotein antibody (50-100) {FITC} (STJ501653)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Mouse

$713.48

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Immunofluorescence of a section of rat cerebellum showing labeling of axons of granule cell and dendrites of Purkinje with alpha-internexin (STJA0003573, 1:5000, red) , labeling of the myelin sheaths around the axons with MBP (STJA0003695, 1:5000, green) , and additional nuclear staining with DAPI (blue).

Anti-Alpha Internexin/NF-66 antibody (STJA0003573)

Clonality :

Polyclonal

Host :

Chicken

Applications :

WB/IHC/ICC

Reactivity :

Bovine/Human/Mouse/Rat

$544.73

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Anti-alpha Synuclein antibody (C-Term) (STJ13100579)

Clonality :

Polyclonal

Host :

Goat

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

$447.53

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Anti-Alpha Synuclein antibody (C-Term) (STJ13100584)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

$447.53

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Western blot analysis of 1) Mouse skeletal muscle tissue, 2) Mouse kidney tissue, 3) Mouse Heart Tissue, 4) Mouse brain tissue, 5) Mouse Liver tissue, diluted at 1:2000. Secondary antibody (STJ99666) was diluted at 1:20000

Anti-Alpha Tubulin antibody (STJ197321)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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STJ73368 (0. 3µg/ml) staining of albumin-depleted Serum (A) and Plasma (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-alpha-1-antichymotrypsin antibody (222-236) (STJ73368)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

$421.88

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STJ73055 (0. 01µg/ml) staining of Albumin-depleted Plasma (A) and Serum (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-alpha-1-antitrypsin antibody (230-241) (STJ73055)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

$421.88

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STJ73054 (0. 3µg/ml) staining of Albumin-depleted Human Plasma lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-alpha-1-antitrypsin antibody (Internal) (STJ73054)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

$421.88

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STJ73369 (0. 5µg/ml) staining of Human Albumin-depleted Serum lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-alpha-2-macroglobulin antibody (Internal) (STJ73369)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human

$421.88

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STJ73360 (0. 3µg/ml) staining of HepG2 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-alpha-fetoprotein antibody (68-82) (STJ73360)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human

$421.88

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Anti-Alpha-Fetoprotein antibody (STJ16101352)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

$625.73 - $839.03

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STJ72875 (0. 1µg/ml) staining of Human Amygdala (A) and fetal Mouse (B) Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Alpha-synuclein antibody (Internal) (STJ72875)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog/Pig

$421.88

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STJ72876 (0. 1µg/ml) staining of Human Amygdala lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Alpha-synuclein antibody (Internal) (STJ72876)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Pig

$421.88

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Western blot analysis of various lysates using Alpha-Tubulin Rabbit pAb (STJ11103685) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Alpha-Tubulin antibody (1-180) (STJ11103685)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Mouse/Rat

$216.68 - $405.68

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Western blot analysis of various lysates using Alpha-tubulin (ubiquitous) chain Rabbit pAb (STJ11103689) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Alpha-tubulin antibody (1-80) (STJ11103689)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC

Reactivity :

Human/Mouse/Rat

$130.28 - $529.88

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Western blot analysis of various lysates using Alpha-tubulin (ubiquitous) chain Rabbit pAb (STJ11103690) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Alpha-tubulin antibody (1-80) (STJ11103690)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Mouse/Rat

$280.13 - $529.88

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STJ71657 (0. 1µg/ml) staining of HepG2 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Alpha2-antiplasmin antibody (Internal) (STJ71657)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF/FC

Reactivity :

Human

$421.88

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Western blot analysis of 293F-ALPI, using ALPI rabbit polyclonal antibody (STJ27982) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ALPI antibody (20-527) (STJ27982)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Anti-ALPI antibody (Internal) (STJ73790)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC

Reactivity :

Human

$421.88

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Western blot analysis of lysates from wild type (WT) and 293T cells transfected with ALPK1 using ALPK1 Rabbit pAb (STJ11106978) at1:1000 dilution. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 10s. <br><b>WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS</b>

Anti-ALPK1 antibody (1-473 aa) (STJ11106978)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

$151.88 - $708.08

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Western blot analysis of various cells using Alpha-protein Kinase 1 Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Anti-ALPK1 antibody (11-60 aa) (STJ96366)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of various lysates using ALPK1 Rabbit polyclonal antibody (STJ11103266) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ALPK1 antibody (340-500) (STJ11103266)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$151.88 - $708.08

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Immunohistochemistry analysis of paraffin-embedded human heart, using ALPK2 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-ALPK2 antibody (1741-1790 aa) (STJ91565)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Anti-ALPK3 antibody (730-810 aa) (STJ190245)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Immunofluorescence analysis of Human colon cancer tissue using Alkaline Phosphatase (ALPL) Rabbit polyclonal antibody (STJ22595) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining.

Anti-ALPL antibody (120-260) (STJ22595)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of the lysates from Jurkat cells using ALPL antibody.

Anti-ALPL antibody (201-250 aa) (STJ96048)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunohistochemistry analysis of paraffin-embedded mouse kidney using Alkaline Phosphatase (Alkaline Phosphatase (ALPL) ) Rabbit polyclonal antibody (STJ114272) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-ALPL antibody (230-440) (STJ114272)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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STJ73461 (0. 1µg/ml) staining of Human Kidney (A) , Lung (B) and Adrenal Gland (C) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-ALPL antibody (42-53) (STJ73461)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat

$421.88

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Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells, using 3 Mu g Placental alkaline phosphatase (PLAP) antibody (STJ11104336). Western blot was performed from the immunoprecipitate using Placental alkaline phosphatase (PLAP) antibody (STJ11104336) at a dilution of 1:1000.

Anti-ALPP antibody (260-460) (STJ11104336)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

$151.88 - $708.08

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Anti-ALPP antibody [ABT73R] (STJ197054)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human

$234.90 - $340.20

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Western blot analysis of 293 cells using ALPP/ALPPL2 Polyclonal Antibody. Antibody was diluted at 1:2000. Secondary antibody was diluted at 1:20000

Anti-ALPP/ALPPL2 antibody (1-50 aa) (STJ96740)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using ALPPL2 antibody (STJ28946) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-ALPPL2 antibody (20-280) (STJ28946)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Anti-ALS antibody (C-Term) (STJ71909)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA

Reactivity :

Human/Mouse/Rat/Dog/Cow/Pig

$261.23

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Immunofluorescence analysis of mouse brain cells using ALS2 Rabbit polyclonal antibody (STJ29205) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ALS2 antibody (260-429) (STJ29205)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-ALS2 antibody (390-470 aa) (STJ190540)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-ALS2 antibody (N-Term) (STJ13100566)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Rat

$447.53

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Anti-ALS2 antibody (N-Term) (STJ13100567)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Rat

$447.53

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Western blot analysis of lysates from HuvEc cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ALS2CL antibody (606-656 aa) (STJ193682)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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STJ70325 staining (1ug/ml) of Human Heart lysate (RIPA buffer, 30ug total NA NA NA protein per lane). Primary incubated for 1 hour. Detected by western blot NA NA NA using chemiluminescence.

Anti-ALS2CR1/NIF3L1 antibody (C-Term) (STJ70325)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse

$421.88

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Anti-ALS2CR16 antibody (STJA0010102)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

0

$64.80 - $340.20

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STJ70323 (1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-ALS2CR2/ILPIP antibody (C-Term) (STJ70323)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF

Reactivity :

Human/Dog/Cow

$421.88

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STJ70326 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-ALS2CR3/GRIF1 antibody (C-Term) (STJ70326)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/FC/IF

Reactivity :

Human/Mouse/Rat/Pig/Cow

$421.88

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Polyclonal Antibodies