• Western blot analysis of extracts of various cell lines, using ALKBH5 antibody (STJ113284) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded mouse lung using ALKBH5 rabbit polyclonal antibody (STJ113284) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat testis using ALKBH5 rabbit polyclonal antibody (STJ113284) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-ALKBH5 antibody (245-394) (STJ113284)

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STJ113284

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ALKBH5 (245-394) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ALKBH5
Gene ID: 54890
Uniprot ID: ALKB5_HUMAN
Immunogen Region: 245-394
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 245-394 of human ALKBH5 (NP_060228.3).
Immunogen Sequence: SLPVRRGSVTVLSGYAADEI THCIRPQDIKERRAVIILRK TRLDAPRLETKSLSSSVLPP SYASDRLSGNNRDPALKPKR SHRKADPDAAHRPRILEMDK EENRRSVLLPTHRRRGSFSS ENYWRKSYESSEDCSEAAGS PARKVKMRRH
Tissue Specificity Widely expressed, with highest expression in lung, followed by testis, pancreas, spleen and ovary.
Function Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro). Requires molecular oxygen, alpha-ketoglutarate and iron. Demethylation of m6A mRNA affects mRNA processing and export. Required for the late meiotic and haploid phases of spermatogenesis by mediating m6A demethylation in spermatocytes and round spermatids: m6A demethylation of target transcripts is required for correct splicing and the production of longer 3'-UTR mRNAs in male germ cells.
Protein Name Rna Demethylase Alkbh5
Alkylated Dna Repair Protein Alkb Homolog 5
Alpha-Ketoglutarate-Dependent Dioxygenase Alkb Homolog 5
Database Links Reactome: R-HSA-73943
Cellular Localisation Nucleus Speckle
Alternative Antibody Names Anti-Rna Demethylase Alkbh5 antibody
Anti-Alkylated Dna Repair Protein Alkb Homolog 5 antibody
Anti-Alpha-Ketoglutarate-Dependent Dioxygenase Alkb Homolog 5 antibody
Anti-ALKBH5 antibody
Anti-ABH5 antibody
Anti-OFOXD1 antibody

Information sourced from Uniprot.org

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