• Immunohistochemistry analysis of paraffin-embedded human gastric cancer using ALKBH8 antibody (STJ29222) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse lung using ALKBH8 antibody (STJ29222) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using ALKBH8 Rabbit polyclonal antibody (STJ29222) at 1:14000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ALKBH8 antibody (1-230) (STJ29222)

SKU:
STJ29222

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ALKBH8 (1-230) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:10000
IHC-P 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ALKBH8
Gene ID: 91801
Uniprot ID: ALKB8_HUMAN
Immunogen Region: 1-230
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human ALKBH8 (NP_620130.2).
Immunogen Sequence: MDSNHQSNYKLSKTEKKFLR KQIKAKHTLLRHEGIETVSY ATQSLVVANGGLGNGVSRNQ LLPVLEKCGLVDALLMPPNK PYSFARYRTTEESKRAYVTL NGKEVVDDLGQKITLYLNFV EKVQWKELRPQALPPGLMVV EEIISSEEEKMLLESVDWTE DTDNQNSQKSLKHRRVKHFG YEFHYENNNVDKDKPLSGGL PDICESFLEKWLRKGYIKHK PDQMTINQYE
Tissue Specificity Widely expressed, with highest expression in spleen, followed by pancreas and lung.
Function Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its methyltransferase domain. Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA. Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys). Binds tRNA and catalyzes the iron and alpha-ketoglutarate dependent hydroxylation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its dioxygenase domain, giving rise to 5-(S)-methoxycarbonylhydroxymethyluridine.has a preference for tRNA(Gly). Required for normal survival after DNA damage. May inhibit apoptosis and promote cell survival and angiogenesis.
Protein Name Alkylated Dna Repair Protein Alkb Homolog 8
Probable Alpha-Ketoglutarate-Dependent Dioxygenase Abh8
S-Adenosyl-L-Methionine-Dependent Trna Methyltransferase Abh8
Trna
Carboxymethyluridine(34-5-O-Methyltransferase Abh8
Database Links Reactome: R-HSA-6782315
Cellular Localisation Cytoplasm
Nucleus
Predominantly Cytoplasmic
Alternative Antibody Names Anti-Alkylated Dna Repair Protein Alkb Homolog 8 antibody
Anti-Probable Alpha-Ketoglutarate-Dependent Dioxygenase Abh8 antibody
Anti-S-Adenosyl-L-Methionine-Dependent Trna Methyltransferase Abh8 antibody
Anti-Trna antibody
Anti-Carboxymethyluridine(34-5-O-Methyltransferase Abh8 antibody
Anti-ALKBH8 antibody
Anti-ABH8 antibody

Information sourced from Uniprot.org

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