Anti-ALKBH4 antibody (1-302) (STJ114467)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ALKBH4 (1-302) antibody for use in WB, IF, ICC and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ALKBH4 |
| Gene ID | 54784 |
| Uniprot ID | ALKB4_HUMAN |
| Immunogen Region | 1-302 |
| Immunogen Sequence | MAAAAAETPEVLRECGCKGI RTCLICERQRGSDPPWELPP AKTYRFIYCSDTGWAVGTEE SDFEGWAFPFPGVMLIEDFV TREEEAELVRLMDRDPWKLS QSGRRKQDYGPKVNFRKQKL KTEGFCGLPSFSREVVRRMG LYPGLEGFRPVEQCNLDYCP ERGSAIDPHLDDAWLWGERL VSLNLLSPTVLSMCREAPGS LLLCSAPSAAPEALVDSVIA PSRSVLCQEVEVAIPLPAR |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-302 of human ALKBH4 (NP_060091.1). |
Additional Info
| Tissue Specificity | Widely expressed, with highest expression in pancreas, ovary and spleen. |
| Function | Dioxygenase that mediates demethylation of actin monomethylated at 'Lys-84' (K84me1), thereby acting as a regulator of actomyosin-processes. Demethylation of actin K84me1 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. In addition to proteins, also demethylates DNA: specifically demethylates DNA methylated on the 6th position of adenine (N(6)-methyladenosine) DNA, thereby regulating Polycomb silencing. |
| Protein Name | Alpha-Ketoglutarate-Dependent Dioxygenase Alkb Homolog 4Alkylated Dna Repair Protein Alkb Homolog 4Dna N6-Methyl Adenine Demethylase Alkbh4Lysine-Specific Demethylase Alkbh4 |
| Database Links | |
| Cellular Localisation | CytoplasmNucleusNucleolusMidbodyAssociates With The Contractile Ring And Midbody |
| Alternative Antibody Names | Anti-Alpha-Ketoglutarate-Dependent Dioxygenase Alkb Homolog 4 antibodyAnti-Alkylated Dna Repair Protein Alkb Homolog 4 antibodyAnti-Dna N6-Methyl Adenine Demethylase Alkbh4 antibodyAnti-Lysine-Specific Demethylase Alkbh4 antibodyAnti-ALKBH4 antibodyAnti-ABH4 antibody |
Information sourced from Uniprot.org