Western Blot Antibodies | St John's Laboratory

Western blot analysis of lysates from wild type (WT) and AIP knockout (KO) HeLa cells, using [KO Validated] AIP Rabbit polyclonal antibody (STJ11100845) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-AIP antibody (1-330) (STJ11100845)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Western blot analysis of extracts of various cell lines, using AIP antibody (STJ114420) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-AIP antibody (1-330) (STJ114420)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of lysates from VEC cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-AIP antibody (251-301 aa) (STJ194443)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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STJ71174 (0. 02µg/ml) staining of NIH/3T3 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-AIP/ARA9 antibody (C-Term) (STJ71174)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog

€390.63

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Anti-AIP1 antibody (1050-1100 aa) (STJ13100692)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

€414.38

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Anti-AIP1 antibody (1260-1310 aa) (STJ13100693)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

€414.38

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Immunofluorescence analysis of Y79 cells using AIPL1 antibody (STJ116048) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-AIPL1 antibody (1-384) (STJ116048)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunofluorescence analysis of Y79 cells using AIPL1 antibody (STJ28541) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-AIPL1 antibody (1-384) (STJ28541)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Anti-AIPL1 antibody (Internal Region) (STJ13100581)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

€414.38

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Anti-AIPL1 antibody (STJA0008194)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of various lysates using AIRE Rabbit pAb (STJ116116) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-AIRE antibody (1-280) (STJ116116)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-AIRE antibody (100-150 aa) (STJ13100688)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat

€414.38

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Western blot analysis of lysates from HeLa cells, treated with Hu 2nM 24h, using AIRE Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-AIRE antibody (126-175 aa) (STJ91519)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Immunofluorescence analysis of C6 cells using AIRE Rabbit polyclonal antibody (STJ22559) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-AIRE antibody (30-130) (STJ22559)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-AIRE antibody (30-80 aa) (STJ13100687)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

€414.38

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Western blot analysis of various cells using AIRE-1 Polyclonal Antibody diluted at 1：1000

Anti-AIRE antibody (91-140 aa) (STJ91518)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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STJ70203 (1µg/ml) staining of nuclear Jurkat cell (A) , (2ug/ml) Human Spleen (B) and negative control Human Smooth Muscle (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-AIRE antibody (C-Term) (STJ70203)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF/FC

Reactivity :

Human

€390.63

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STJ70934 staining (0. 05µg/ml) of HEK293 cell lysates. Untransfected (Lane 3) and transfected with Human AIRE (lane 4). Data kindly provided by Prof. Pärt Peterson, University of Tartu, Estonia.

Anti-AIRE antibody (Internal) (STJ70934)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Rat/Dog/Cow

€390.63

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STJ71077 (0. 3µg/ml) staining of human spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-AIRE antibody (Internal) (STJ71077)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Rat/Pig/Cow

€390.63

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Western blot analysis of Rat kidney, using Ajuba Rabbit polyclonal antibody antibody (STJ11104525) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-AJUBA antibody (201-290) (STJ11104525)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

€161.88 - €803.13

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Anti-AJUBA antibody (STJA0008669)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunohistochemistry analysis of paraffin-embedded mouse heart using AK1 Antibody (STJ115421) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-AK1 antibody (1-194) (STJ115421)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunofluorescence analysis of HeLa cells using AK1 antibody (STJ22560). Blue: DAPI for nuclear staining.

Anti-AK1 antibody (1-194) (STJ22560)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Western blot analysis of extracts of HeLa cells, using AK2 antibody (STJ11105685). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-AK2 antibody (1-232) (STJ11105685)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Immunohistochemistry analysis of paraffin-embedded human gastric cancer using AK2 antibody (STJ28602) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-AK2 antibody (1-232) (STJ28602)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Immunocytochemistry analysis of AK2 (green) in Hela using AK2 antibody, and DAPI (blue).

Anti-AK2 antibody (171-184 aa) [R07-4H8] (STJA0012261)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

€330.63

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Anti-AK2 antibody (STJA0007242)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of various lysates using AK3 Rabbit polyclonal antibody (STJ116255) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-AK3 antibody (1-227) (STJ116255)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-AK3 antibody (STJA0008341)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of lysates from wild type (WT) and AK4 knockout (KO) HeLa cells, using [KO Validated] AK4 Rabbit polyclonal antibody (STJ11100730) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-AK4 antibody (1-223) (STJ11100730)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€161.88 - €803.13

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Immunoprecipitation analysis of 200 Mu g extracts of HepG2 cells, using 3 Mu g AK4 antibody (STJ115422). Western blot was performed from the immunoprecipitate using AK4 antibody (STJ115422) at a dilution of 1:1000.

Anti-AK4 antibody (1-223) (STJ115422)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Immunoprecipitation analysis of 200 Mu g extracts of HepG2 cells, using 3 Mu g AK4 antibody (STJ22562). Western blot was performed from the immunoprecipitate using AK4 antibody (STJ22562) at a dilution of 1:1000.

Anti-AK4 antibody (1-223) (STJ22562)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-AK4 antibody (STJA0008417)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunohistochemistry analysis of AK5 in paraffin-embedded rat brain using AK5 Rabbit polyclonal antibody (STJ117619) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-AK5 antibody (150-370) (STJ117619)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various cells using AK5 Polyclonal Antibody

Anti-AK5 antibody (391-440 aa) (STJ91522)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of various cells using AK6 Polyclonal Antibody

Anti-AK6 antibody (11-60 aa) (STJ91523)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of various lysates using AK7 Rabbit polyclonal antibody (STJ116810) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-AK7 antibody (1-200) (STJ116810)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various lysates using AK8 Rabbit pAb (STJ115709) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 90s.

Anti-AK8 antibody (1-200) (STJ115709)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

€140.63 - €655.63

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Anti-AK8 antibody (370-450 aa) (STJ191784)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Anti-AK9 antibody (STJA0008625)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

€60.00 - €315.00

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Western blot analysis of various cells using AKAP 149 Polyclonal Antibody diluted at 1：2000

Anti-AKAP1 antibody (281-330 aa) (STJ91528)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of various lysates using AKAP1 Rabbit pAb (STJ11107422). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST.

Anti-AKAP1 antibody (30-280 aa) (STJ11107422)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/FC/ELISA

Reactivity :

Human

€140.63 - €655.63

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Western blot analysis of mouse cells using AKAP 10 Polyclonal Antibody

Anti-AKAP10 antibody (10-59 aa) (STJ91524)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-AKAP10 antibody (413-662 aa) (STJ11107407)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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STJ71613 (1µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-AKAP10 antibody (Internal) (STJ71613)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Cow

€390.63

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STJ70532 (0. 3ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer. Primary incubation was 1 hour. Deected by chemiluminescence.

Anti-AKAP10/D-AKAP2 antibody (C-Term) (STJ70532)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog

€390.63

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Anti-AKAP12 antibody (680-900 aa) (STJ11106438)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Anti-AKAP13 antibody (1-150 aa) (STJ11107463)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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