Anti-AFF4 antibody (1-220) (STJ22540)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal AFF4 (1-220) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | AFF4 |
| Gene ID | 27125 |
| Uniprot ID | AFF4_HUMAN |
| Immunogen Region | 1-220 |
| Immunogen Sequence | MNREDRNVLRMKERERRNQE IQQGEDAFPPSSPLFAEPYK VTSKEDKLSSRIQSMLGNYD EMKDFIGDRSIPKLVAIPKP TVPPSADEKSNPNFFEQRHG GSHQSSKWTPVGPAPSTSQS QKRSSGLQSGHSSQRTSAGS SSGTNSSGQRHDRESYNNSG SSSRKKGQHGSEHSKSRSSS PGKPQAVSSLNSSHSRSHGN DHHSKEHQRSKSPRDPDANW |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human AFF4 (NP_055238.1). |
Additional Info
| Tissue Specificity | Ubiquitously expressed. Strongly expressed in heart, placenta, skeletal muscle, pancreas and to a lower extent in brain. |
| Post Translational Modifications | Dephosphorylated at Ser-549 by the PNUTS-PP1 complex, promoting RNA polymerase II transcription pause-release. |
| Function | Key component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. In the SEC complex, AFF4 acts as a central scaffold that recruits other factors through direct interactions with ELL proteins (ELL, ELL2 or ELL3) and the P-TEFb complex. In case of infection by HIV-1 virus, the SEC complex is recruited by the viral Tat protein to stimulate viral gene expression. |
| Protein Name | Af4/Fmr2 Family Member 4All1-Fused Gene From Chromosome 5q31 ProteinProtein Af-5q31Major Cdk9 Elongation Factor-Associated Protein |
| Database Links | Reactome: R-HSA-112382Reactome: R-HSA-674695Reactome: R-HSA-75955 |
| Cellular Localisation | NucleusChromosomeAssociates To Transcriptionally Active Chromatin But Not At Snrna Genes |
| Alternative Antibody Names | Anti-Af4/Fmr2 Family Member 4 antibodyAnti-All1-Fused Gene From Chromosome 5q31 Protein antibodyAnti-Protein Af-5q31 antibodyAnti-Major Cdk9 Elongation Factor-Associated Protein antibodyAnti-AFF4 antibodyAnti-AF5Q31 antibodyAnti-MCEF antibodyAnti-HSPC092 antibody |
Information sourced from Uniprot.org