• Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g AFG3L2 antibody (STJ117588). Western blot was performed from the immunoprecipitate using AFG3L2 antibody (STJ117588) at a dilution of 1:1000.
  • Immunohistochemistry analysis of AFG3L2 in paraffin-embedded human colon carcinoma using AFG3L2 Rabbit polyclonal antibody (STJ117588) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using AFG3L2 Rabbit polyclonal antibody (STJ117588) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-AFG3L2 antibody (538-797) (STJ117588)

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STJ117588

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AFG3L2 (538-797) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AFG3L2
Gene ID: 10939
Uniprot ID: AFG32_HUMAN
Immunogen Region: 538-797
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 538-797 of human AFG3L2 (NP_006787.2).
Immunogen Sequence: DSINQKHFEQAIERVIGGLE KKTQVLQPEEKKTVAYHEAG HAVAGWYLEHADPLLKVSII PRGKGLGYAQYLPKEQYLYT KEQLLDRMCMTLGGRVSEEI FFGRITTGAQDDLRKVTQSA YAQIVQFGMNEKVGQISFDL PRQGDMVLEKPYSEATARLI DDEVRILINDAYKRTVALLT EKKADVEKVALLLLEKEVLD KNDMVELLGPRPFAEKSTYE EFVEGTGSLDEDTSLPEGL
Tissue Specificity Ubiquitous. Highly expressed in the cerebellar Purkinje cells.
Post Translational Modifications Upon import into the mitochondrion, the N-terminal transit peptide is cleaved to generate an intermediate form which undergoes autocatalytic proteolytic processing to generate the proteolytically active mature form.
Function ATP-dependent protease which is essential for axonal and neuron development. In neurons, mediates degradation of SMDT1/EMRE before its assembly with the uniporter complex, limiting the availability of SMDT1/EMRE for MCU assembly and promoting efficient assembly of gatekeeper subunits with MCU. Required for paraplegin (SPG7) maturation. After its cleavage by mitochondrial-processing peptidase (MPP), it converts paraplegin into a proteolytically active mature form. Required for the maturation of PINK1 into its 52kDa mature form after its cleavage by mitochondrial-processing peptidase (MPP). Involved in the regulation of OMA1-dependent processing of OPA1. Contributes to the proteolytic degradation of GHITM upon hyperpolarization of mitochondria. Progressive GHITM degradation upon persistent hyperpolarization leads to respiratory complex I degradation and broad reshaping of the mitochondrial proteome by AFG3L2.
Protein Name Afg3-Like Protein 2
Paraplegin-Like Protein
Database Links Reactome: R-HSA-8949664
Cellular Localisation Mitochondrion
Mitochondrion Inner Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Afg3-Like Protein 2 antibody
Anti-Paraplegin-Like Protein antibody
Anti-AFG3L2 antibody

Information sourced from Uniprot.org

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