• Western blot analysis of mouse testis, using ADSL rabbit polyclonal antibody (STJ28200) at 1:700 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
  • Immunofluorescence analysis of HeLa cells using ADSL antibody (STJ28200). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using ADSL rabbit polyclonal antibody (STJ28200) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using ADSL rabbit polyclonal antibody (STJ28200) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using ADSL rabbit polyclonal antibody (STJ28200) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-ADSL antibody (1-310) (STJ28200)

SKU:
STJ28200

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ADSL (1-310) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:20-1:100
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ADSL
Gene ID: 158
Uniprot ID: PUR8_HUMAN
Immunogen Region: 1-310
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human ADSL (NP_000017.1).
Immunogen Sequence: MAAGGDHGSPDSYRSPLASR YASPEMCFVFSDRYKFRTWR QLWLWLAEAEQTLGLPITDE QIQEMKSNLENIDFKMAAEE EKRLRHDVMAHVHTFGHCCP KAAGIIHLGATSCYVGDNTD LIILRNALDLLLPKLARVIS RLADFAKERASLPTLGFTHF QPAQLTTVGKRCCLWIQDLC MDLQNLKRVRDDLRFRGVKG TTGTQASFLQLFEGDDHKVE QLDKMVTEKAGFKRAFIIT
Tissue Specificity Ubiquitously expressed. Both isoforms are produced by all tissues. Isoform 2 is 10-fold less abundant than isoform 1.
Function Catalyzes two non-sequential steps in de novo AMP synthesis: converts (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate (SAICAR) to fumarate plus 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide, and thereby also contributes to de novo IMP synthesis, and converts succinyladenosine monophosphate (SAMP) to AMP and fumarate.
Protein Name Adenylosuccinate Lyase
Adsl
Asl
Adenylosuccinase
Asase
Database Links Reactome: R-HSA-73817
Alternative Antibody Names Anti-Adenylosuccinate Lyase antibody
Anti-Adsl antibody
Anti-Asl antibody
Anti-Adenylosuccinase antibody
Anti-Asase antibody
Anti-ADSL antibody
Anti-AMPS antibody

Information sourced from Uniprot.org

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