• Western blot analysis of extracts of various cell lines, using AES antibody (STJ110184) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using AES antibody (STJ110184) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-AES antibody (1-264) (STJ110184)

SKU:
STJ110184

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AES (1-264) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TLE5
Gene ID: 166
Uniprot ID: TLE5_HUMAN
Immunogen Region: 1-264
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human AES (NP_945320.1).
Immunogen Sequence: MCHKNGFPQEGGITAAFLQK RKLRLSKNHRPARAKVTEHV RGTRPGRATAGPAASTRAAG SLFFDRWGNRGPAGCRGSSH LPQQLKFTTSDSCDRIKDEF QLLQAQYHSLKLECDKLASE KSEMQRHYVMYYEMSYGLNI EMHKQAEIVKRLNGICAQVL PYLSQEHQQQVLGAIERAKQ VTAPELNSIIRQQLQAHQLS QLQALALPLTPLPVGLQPPS LPAVSAGTGLLSLSALGSQ
Tissue Specificity Found predominantly in muscle, heart and Placenta. In fetal tissues, abundantly expressed in the heart, lung, kidney, brain and liver.
Post Translational Modifications Ubiquitinated by XIAP/BIRC4.
Function Transcriptional corepressor. Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Essential for the transcriptional repressor activity of SIX3 during retina and lens development.
Protein Name Tle Family Member 5
Amino-Terminal Enhancer Of Split
Amino Enhancer Of Split
Gp130-Associated Protein Gam
Grg-5
Groucho-Related Protein 5
Protein Esp1
Protein Grg
Tle Family Member 5 - Transcriptional Modulator
Database Links Reactome: R-HSA-4641265
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Tle Family Member 5 antibody
Anti-Amino-Terminal Enhancer Of Split antibody
Anti-Amino Enhancer Of Split antibody
Anti-Gp130-Associated Protein Gam antibody
Anti-Grg-5 antibody
Anti-Groucho-Related Protein 5 antibody
Anti-Protein Esp1 antibody
Anti-Protein Grg antibody
Anti-Tle Family Member 5 - Transcriptional Modulator antibody
Anti-TLE5 antibody
Anti-AES antibody
Anti-GRG antibody
Anti-GRG5 antibody

Information sourced from Uniprot.org

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