• Immunohistochemistry analysis of paraffin-embedded mouse brain using AEBP2 antibody (STJ29537) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using AEBP2 antibody (STJ29537) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using AEBP2 antibody (STJ29537) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s.

Anti-AEBP2 antibody (234-503) (STJ29537)

SKU:
STJ29537

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AEBP2 (234-503) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AEBP2
Gene ID: 121536
Uniprot ID: AEBP2_HUMAN
Immunogen Region: 234-503
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 234-503 of human AEBP2 (NP_694939.2).
Immunogen Sequence: STISSGRSTPAMMNGQGSTT SSSKNIAYNCCWDQCQACFN SSPDLADHIRSIHVDGQRGG VFVCLWKGCKVYNTPSTSQS WLQRHMLTHSGDKPFKCVVG GCNASFASQGGLARHVPTHF SQQNSSKVSSQPKAKEESPS KAGMNKRRKLKNKRRRSLPR PHDFFDAQTLDAIRHRAICF NLSAHIESLGKGHSVVFHST VIAKRKEDSGKIKLLLHWMP EDILPDVWVNESERHQLKT
Function Acts as an accessory subunit for the core Polycomb repressive complex 2 (PRC2), which mediates histone H3K27 (H3K27me3) trimethylation on chromatin leading to transcriptional repression of the affected target gene. Plays a role in nucleosome localization of the PRC2 complex.
Protein Name Zinc Finger Protein Aebp2
Adipocyte Enhancer-Binding Protein 2
Ae-Binding Protein 2
Database Links Reactome: R-HSA-212300
Reactome: R-HSA-3214841
Cellular Localisation Nucleus
Localizes To Chromatin As Part Of The Prc2 Complex
Alternative Antibody Names Anti-Zinc Finger Protein Aebp2 antibody
Anti-Adipocyte Enhancer-Binding Protein 2 antibody
Anti-Ae-Binding Protein 2 antibody
Anti-AEBP2 antibody

Information sourced from Uniprot.org

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