Western Blot Antibodies | St John's Laboratory

Western blot analysis of various cells using p39 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-CDK5R2 antibody (81-130 aa) (STJ94880)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Immunohistochemistry analysis of CDK5RAP1 in paraffin-embedded mouse kidney using CDK5RAP1 Rabbit polyclonal antibody (STJ118289) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CDK5RAP1 antibody (1-350) (STJ118289)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various lysates using CDK5RAP2 Rabbit polyclonal antibody (STJ117671) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-CDK5RAP2 antibody (1744-1893) (STJ117671)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Western blot analysis of various lysates using CDK5RAP3 Rabbit polyclonal antibody (STJ115094) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CDK5RAP3 antibody (1-300) (STJ115094)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Western blot analysis of various lysates using CDK5RAP3 Rabbit polyclonal antibody (STJ116272) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-CDK5RAP3 antibody (1-300) (STJ116272)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various cells using HSF-27 Polyclonal Antibody diluted at 1：2000

Anti-CDK5RAP3 antibody (341-390 aa) (STJ93603)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunofluorescence analysis of mouse brain using CDK6 antibody (STJ118785) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-CDK6 antibody (1-326) (STJ118785)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various lysates using CDK6 Rabbit pAb (STJ11106790) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 90s.

Anti-CDK6 antibody (226-326 aa) (STJ11106790)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunoprecipitation analysis of 200 Mu g extracts of Jurkat cells, using 3 Mu g CDK6 antibody (STJ11103846). Western blot was performed from the immunoprecipitate using CDK6 antibody (STJ11103846) at a dilution of 1:1000.

Anti-CDK6 antibody (226-326) (STJ11103846)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Western blot analysis of extracts of various cell lines, using CDK6 Rabbit monoclonal antibody (STJ11101226) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-CDK6 antibody (227-326) [S6MR] (STJ11101226)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

€161.88 - €718.13

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Western blot analysis of 293T Hela lysate, antibody was diluted at 2000. Secondary antibody was diluted at 1:20000

Anti-CDK6 antibody (280-325 aa) (STJ98721)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Immunofluorescence analysis of HeLa cells using CDK7 Rabbit polyclonal antibody (STJ23066). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CDK7 antibody (1-346) (STJ23066)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Western blot analysis of lysates from wild type (WT) and CDK7 knockout (KO) 293T cells, using [KO Validated] CDK7 Rabbit polyclonal antibody (STJ114808) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CDK7 antibody (211-346) (STJ114808)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Western blot analysis of various cells using Cdk7 Polyclonal Antibody

Anti-CDK7 antibody (291-340 aa) (STJ92203)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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STJ73141 (0. 3µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CDK7 antibody (47-58) (STJ73141)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Cow

€390.63

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Western blot analysis of various lysates using CDK8 Rabbit mAb (STJ11102517) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-CDK8 antibody (1-100) [S7MR] (STJ11102517)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

€161.88 - €718.13

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Western blot analysis of various cells using Cdk8 Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-CDK8 antibody (1-50 aa) (STJ92205)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of extracts of various lysates, using CDK8 Rabbit monoclonal antibody (STJ11105214) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CDK8 antibody (364-464 aa) [S5214RM] (STJ11105214)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€161.88 - €718.13

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Immunoprecipitation analysis of 200 Mu g extracts of Jurkat cells, using 3 Mu g CDK8 antibody (STJ27494). Western blot was performed from the immunoprecipitate using CDK8 antibody (STJ27494) at a dilution of 1:1000.

Anti-CDK8 antibody (365-464) (STJ27494)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Western blot analysis of extracts of C6 cells, using CDK9 antibody (STJ11102769) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CDK9 antibody (153-372) (STJ11102769)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunohistochemistry analysis of paraffin-embedded rat liver using CDK9 Rabbit polyclonal antibody (STJ114907) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CDK9 antibody (153-372) (STJ114907)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K562 cells with 1 Mu g CDK9 rabbit monoclonal antibody (STJ11101280) , along with a Goat Anti-rabbit IgG (H+L). The CUT&Tag results indicate the enrichment pattern of CDK9 in representative gene loci (RAC1) , as shown in figure.

Anti-CDK9 antibody (273-372) [S0MR] (STJ11101280)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€183.13 - €803.13

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STJ73577 (2µg/ml) staining of Mouse Skeletal Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CDK9 antibody (86-98) (STJ73577)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

€390.63

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STJ73576 (0. 5µg/ml) staining of NIH3T3 nuclear lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CDK9 antibody (C-Term) (STJ73576)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

€390.63

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Anti-CDK9 antibody (STJA0010131)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis using Cdk9 monoclonal antibody against HEK293 (1) and CDK9-hIgGFc transfected HEK293 (2) cell lysate.

Anti-CDK9 antibody [1B5A7] (STJ97949)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

€180.00 - €435.00

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Anti-CDKAL1 antibody (1-100 aa) (STJ11106050)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Immunofluorescence analysis of U2OS cells using CDKAL1 antibody (STJ28338). Blue: DAPI for nuclear staining.

Anti-CDKAL1 antibody (1-230) (STJ28338)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Western blot analysis of CoLo cells using CDKAL1 Polyclonal Antibody

Anti-CDKAL1 antibody (10-59 aa) (STJ92206)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

€60.00 - €315.00

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Anti-CDKAL1 antibody (Internal) (STJ71432)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog

€390.63

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Western blot analysis of various lysates using Cdkl1 Rabbit pAb (STJ11106605) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Cdkl1 antibody (220-352 aa) (STJ11106605)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various cells using KKIALRE Polyclonal Antibody diluted at 1：500

Anti-CDKL1 antibody (281-330 aa) (STJ93846)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat

€60.00 - €315.00

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Western blot analysis of lysates from Jurkat and 293 cells, using CDKL2 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-CDKL2 antibody (211-260 aa) (STJ93847)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Immunohistochemistry analysis of CDKL3 in paraffin-embedded Human colon carcinoma using CDKL3 Rabbit polyclonal antibody (STJ119103) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CDKL3 antibody (1-120) (STJ119103)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using CDKL3 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-CDKL3 antibody (291-340) (STJ94499)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Anti-CDKL3 antibody (C-Term) (STJA0005700)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

€569.38

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Anti-CDKL3 antibody (N-Term) (STJA0005699)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

€569.38

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Western blot analysis of various cells using CdkL4 Polyclonal Antibody diluted at 1：500

Anti-CDKL4 antibody (266-315 aa) (STJ92207)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Anti-CDKL5 antibody (30-110 aa) (STJ190188)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Whole cell lysates of A549 were separated by 12% SDS-PAGE, and the membrane was blotted with anti-p21 (PTR2559) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: A549Predicted band size: 21kDaObserved band size: 21kDa

Anti-CDKN1A antibody (1-100 aa) (STJ196154)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of extracts of various cell lines, using CDKN1A antibody (STJ11103324) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-CDKN1A antibody (1-159) (STJ11103324)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various lysates, using CDKN1A/p21CIP1 Rabbit polyclonal antibody (STJ11104536) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer:3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CDKN1A antibody (1-164) (STJ11104536)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Immunohistochemistry analysis of CDKN1A/p21CIP1 in paraffin-embedded human breast cancer using CDKN1A/p21CIP1 Rabbit polyclonal antibody (STJ111165) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CDKN1A antibody (1-164) (STJ111165)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Western blot analysis of MCF7, using CDKN1A/p21CIP1 antibody (STJ23069) at 1:900 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CDKN1A antibody (1-164) (STJ23069)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-CDKN1A antibody (1-164) [S1MR] (STJ11101631)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€183.13 - €803.13

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Immunofluorescence analysis of Hela cell. 1, p21 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-CDKN1A antibody (111-160 aa) (STJ94862)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of p21 in MCF-7 lysates using p21 antibody.

Anti-CDKN1A antibody (148-164 aa) [R07-3K5] (STJA0011727)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€308.13

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Anti-CDKN1A antibody (Full Length) [M513] (STJA0005501)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IHC/IP

Reactivity :

Human

€399.38

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