• Immunofluorescence analysis of U2OS cells using Cyclin A2 antibody (STJ29947) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using Cyclin A2 antibody (STJ29947) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human well-differentiated squamous skin carcinoma using Cyclin A2 antibody (STJ29947) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Cyclin A2 antibody (STJ29947) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s.

Anti-CCNA2 antibody (350-432) (STJ29947)

SKU:
STJ29947

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Cyclin A2 (350-432) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CCNA2
Gene ID: 890
Uniprot ID: CCNA2_HUMAN
Immunogen Region: 350-432
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 350-432 of human Cyclin A2 (NP_001228.1).
Immunogen Sequence: YLPSVIAGAAFHLALYTVTG QSWPESLIRKTGYTLESLKP CLMDLHQTYLKAPQHAQQSI REKYKNSKYHGVSLLNPPET LNL
Post Translational Modifications Polyubiquitinated via 'Lys-11'-linked ubiquitin by the anaphase-promoting complex (APC/C), leading to its degradation by the proteasome. Deubiquitinated and stabilized by USP37 enables entry into S phase.
Function Cyclin which controls both the G1/S and the G2/M transition phases of the cell cycle. Functions through the formation of specific serine/threonine protein kinase holoenzyme complexes with the cyclin-dependent protein kinases CDK1 or CDK2. The cyclin subunit confers the substrate specificity of these complexes and differentially interacts with and activates CDK1 and CDK2 throughout the cell cycle.
Protein Name Cyclin-A2
Cyclin-A
Cyclin A
Database Links Reactome: R-HSA-1362300
Reactome: R-HSA-1538133
Reactome: R-HSA-170145
Reactome: R-HSA-171319
Reactome: R-HSA-174184
Reactome: R-HSA-176408
Reactome: R-HSA-187577
Reactome: R-HSA-2559582
Reactome: R-HSA-2559586
Reactome: R-HSA-5689880
Reactome: R-HSA-5693607
Reactome: R-HSA-6804116
Reactome: R-HSA-6804756
Reactome: R-HSA-6804757
Reactome: R-HSA-68911
Reactome: R-HSA-68949
Reactome: R-HSA-69017
Reactome: R-HSA-69273
Reactome: R-HSA-69563
Reactome: R-HSA-69656
Cellular Localisation Nucleus
Cytoplasm
Exclusively Nuclear During Interphase
Detected In The Nucleus And The Cytoplasm At Prophase
Cytoplasmic When Associated With Scaper
Alternative Antibody Names Anti-Cyclin-A2 antibody
Anti-Cyclin-A antibody
Anti-Cyclin A antibody
Anti-CCNA2 antibody
Anti-CCN1 antibody
Anti-CCNA antibody

Information sourced from Uniprot.org

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