Western Blot Antibodies | St John's Laboratory

Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CCN1 antibody (STJ192107)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-CCN2 antibody (130-210 aa) (STJ193187)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of MCF-7 cells using Nov Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CCN3 antibody (221-270 aa) (STJ96777)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CCN4 antibody (STJ192074)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of Hela Cell Lysate using antibody. Secondary antibody was diluted at 1:20000

Anti-CCN6 antibody (1-50 aa) (STJ98950)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates using Cyclin A1 Rabbit polyclonal antibody (STJ116740) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CCNA1 antibody (350-450) (STJ116740)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various cells using Cyclin A1 Polyclonal Antibody diluted at 1：2000

Anti-CCNA1 antibody (411-460 aa) (STJ92533)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using Cyclin A2 antibody (STJ11101574) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CCNA2 antibody (11-210) [S4MR] (STJ11101574)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunofluorescence analysis of U2OS cells using Cyclin A2 antibody (STJ29947) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-CCNA2 antibody (350-432) (STJ29947)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-CCNA2 antibody (STJA0006733)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from Jurkat cells, treated with UV 15', using Cyclin B1 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-CCNB1 antibody (121-170 aa) (STJ92535)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using Cyclin B1 antibody (STJ11101575) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-CCNB1 antibody (3-177) [S5MR] (STJ11101575)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Western blot analysis of extracts of various cell lines, using Cyclin B1 antibody (STJ118478) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CCNB1 antibody (334-433) (STJ118478)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using Cyclin B1 antibody (STJ22943) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CCNB1 antibody (334-433) (STJ22943)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, Cyclin B1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-CCNB1 antibody (91-140 aa) (STJ92534)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis using Cyclin B1 monoclonal antibody against HeLa (1) , Jurkat (2) , K562 (3) and PC-12 (4) cell lysate.

Anti-CCNB1 antibody [1B10] (STJ97976)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£149.00 - £360.00

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Western blot analysis using Cyclin B1 monoclonal antibody against HeLa (1) and PC-12 (2) cell lysate.

Anti-CCNB1 antibody [5G6] (STJ97977)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/IF/FC/ELISA

Reactivity :

Human/Rat

£149.00 - £360.00

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Western blot analysis of lysates from mouse heart, using CCNB1IP1 Rabbit polyclonal antibody (STJ119107) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-CCNB1IP1 antibody (1-50) (STJ119107)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£110.50 - £505.50

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Western blot analysis of various cells using HEI10 Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-CCNB1IP1 antibody (201-250 aa) (STJ93487)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates using Cyclin B2 Rabbit polyclonal antibody (STJ118891) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CCNB2 antibody (1-100) (STJ118891)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using Cyclin B2 antibody (STJ22944) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CCNB2 antibody (1-100) (STJ22944)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CCNB2 antibody (60-140 aa) (STJ190397)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-CCNB3 antibody (STJ191900)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using Cyclin C antibody (STJ115570) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CCNC antibody (1-283) (STJ115570)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using Cyclin C antibody (STJ28628) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CCNC antibody (1-283) (STJ28628)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from LOVO cells, using Cyclin C Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-CCNC antibody (234-283 aa) (STJ92536)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-CCNC antibody (STJ99629)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry of rat kidney with Anti-CCND1 primary antibody (STJ500672) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-CCND1 antibody (1-50) (STJ500672)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

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Anti-CCND1 antibody (1-50) {Biotin} (STJ500678)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-CCND1 antibody (1-50) {FITC} (STJ500679)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-CCND1 antibody (10-60) (STJ500673)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£400.50

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Anti-CCND1 antibody (10-60) {Biotin} (STJ500680)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-CCND1 antibody (10-60) {FITC} (STJ500681)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-CCND1 Antibody (STJ500674) on rat oesophagus at 1:500 dilution in buffer.

Anti-CCND1 antibody (170-220) (STJ500674)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£400.50

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Anti-CCND1 antibody (170-220) {Biotin} (STJ500682)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-CCND1 antibody (170-220) {FITC} (STJ500683)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Confocal imaging of NIH/3T3 cells using [KO Validated] Cyclin D1 Rabbit monoclonal antibody (STJ11101576, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-CCND1 antibody (196-295) [S6MR] (STJ11101576)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Immunoprecipitation analysis of 200 Mu g extracts of HepG2 cells, using 3 Mu g Cyclin D1 antibody (STJ11101272). Western blot was performed from the immunoprecipitate using Cyclin D1 antibody (STJ11101272) at a dilution of 1:1000.

Anti-CCND1 antibody (200-295) (STJ11101272)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry of rat tissue with Anti-CCND1 primary antibody (STJ500675) in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 10X.

Anti-CCND1 antibody (213-232) (STJ500675)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human

£400.50

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Anti-CCND1 antibody (213-232) {Biotin} (STJ500684)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Rat

£497.50

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Anti-CCND1 antibody (213-232) {FITC} (STJ500685)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Rat

£497.50

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Immunofluorescence analysis of Hela cell. 1, Cyclin D1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). GAPDH monoclonal antibody (2B8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-CCND1 antibody (246-295 aa) (STJ92538)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Pig

£49.00 - £261.00

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Anti-CCND1 antibody (268-295) (STJ500676)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

£400.50

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Anti-CCND1 antibody (268-295) {Biotin} (STJ500686)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

£497.50

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Anti-CCND1 antibody (268-295) {FITC} (STJ500687)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

£497.50

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Anti-CCND1 antibody (280-295) (STJ500677)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

£400.50

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Anti-CCND1 antibody (280-295) {Biotin} (STJ500688)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

£497.50

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Anti-CCND1 antibody (280-295) {FITC} (STJ500689)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

£497.50

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