Western Blot Antibodies | St John's Laboratory

Confocal immunofluorescent analysis of Jurkat cells labeling CD3 (ABT295) Mouse monoclonal antibody followed by Goat Anti- mouse IgG H&L (FITC Conjugate) secondary antibody (green). Counterstained with DAPI (blue). Negative control: PBS was used to instead of primary antibody, followed by Goat Anti- mouse IgG H&L (FITC Conjugate) secondary antibody (green).

Anti-CD3E antibody (1-100 aa) [ABT295] (STJ197119)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/ICC/IF

Reactivity :

Human

£180.00 - £261.00

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Immunofluorescence analysis of RAW264. 7 cells using CD3E Antigen antibody (STJ114289) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-CD3E antibody (111-207) (STJ114289)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of Jurkat cells, using CD3E antibody (STJ22996) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CD3E antibody (111-207) (STJ22996)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/FC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot with Anti-CD3E Antibody (STJ500448) of rat thymus at 1:250 dilution in buffer.

Anti-CD3E antibody (150-200) (STJ500448)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-CD3E antibody (150-200) {Biotin} (STJ500454)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-CD3E antibody (150-200) {FITC} (STJ500455)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western blot analysis of various lysates, using CD3E Rabbit mAb (STJ11106853) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-CD3E antibody (158-207 aa) [SRM5073] (STJ11106853)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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Immunohistochemistry analysis of CD3-Epsilon antibody in paraffin-embedded human brain tissue.

Anti-CD3E antibody (22-71 aa) (STJ92126)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse/Monkey

£49.00 - £261.00

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Anti-CD3E antibody (C-Term) (STJ500449)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

£427.50

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Immunohistochemistry of baboon spleen with Anti-CD3E primary antibody (STJ500450) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-CD3E antibody (C-Term) (STJ500450)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£427.50

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Anti-CD3E antibody (C-Term) {Biotin} (STJ500456)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

£528.50

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Anti-CD3E antibody (C-Term) {Biotin} (STJ500458)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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Anti-CD3E antibody (C-Term) {FITC} (STJ500457)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

£528.50

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Anti-CD3E antibody (C-Term) {FITC} (STJ500459)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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Anti-CD3E antibody (Met1-Asp126) [1A154] (STJA0021979)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

£229.50 - £328.50

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Anti-CD3E antibody (Met1-Asp126) [1A155] (STJA0022011)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

£229.50 - £328.50

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Immunohistochemistry of baboon spleen with Anti-CD3E primary antibody (STJ500447) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-CD3E antibody (N-Term) (STJ500447)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey

£427.50

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Anti-CD3E antibody (N-Term) {Biotin} (STJ500452)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey

£528.50

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Anti-CD3E antibody (N-Term) {FITC} (STJ500453)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey

£528.50

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Anti-CD3E antibody [145-2C11] (STJA0027413)

Clonality :

Monoclonal

Host :

Hamster

Applications :

FC/ICC/IHC/IP/T cell stimulation/T cell depletion/WB

Reactivity :

Mouse

£231.50 - £341.50

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Anti-CD3E antibody [145-2C11] {APC} (STJA0029060)

Clonality :

Monoclonal

Host :

Hamster

Applications :

FC/WB

Reactivity :

Mouse

£282.50 - £423.50

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Anti-CD3E antibody [145-2C11] {FITC} (STJA0028094)

Clonality :

Monoclonal

Host :

Hamster

Applications :

FC/WB

Reactivity :

Mouse

£231.50 - £341.50

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Anti-CD3E antibody [145-2C11] {PerCP} (STJA0030026)

Clonality :

Monoclonal

Host :

Hamster

Applications :

FC/WB

Reactivity :

Mouse

£265.50 - £396.50

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Anti-CD3E antibody [145-2C11] {PE} (STJA0030992)

Clonality :

Monoclonal

Host :

Hamster

Applications :

FC/WB

Reactivity :

Mouse

£282.50 - £423.50

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Anti-CD3E antibody [SRM13366] (STJ11108096)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/mIHC/FC

Reactivity :

Human

£0.00

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Anti-CD3E antibody [UCHT1] (STJA0002232)

Clonality :

Monoclonal

Host :

Mouse

Applications :

Activ/Depletion/Inhibition/FC/IHC/WB

Reactivity :

Human

£93.50 - £188.50

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Anti-CD3E antibody [UCHT1] (STJA0002233)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IHC/WB

Reactivity :

Human

£93.50 - £114.50

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Human peripheral blood lymphocytes stained with CD3E+CD3G Rabbit monoclonal antibody (STJ11103942) (1 Mu g/mL) (orange). Secondary antibody only staining (Blue) and unstained sample (red) were used as a control.

Anti-CD3E+CD3G antibody (1-207) [S3942RM] (STJ11103942)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/FC/ELISA

Reactivity :

Human

£126.50 - £554.50

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STJ73089 (0. 1µg/ml) staining of Jurkat (A) and Molt4 (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CD3E-Cytoplasmic antibody (Internal) (STJ73089)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Rat/Pig

£312.50

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Anti-CD3G antibody (131-181) (STJ500451)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse

£427.50

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Anti-CD3G antibody (131-181) {Biotin} (STJ500466)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse

£528.50

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Anti-CD3G antibody (131-181) {FITC} (STJ500467)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse

£528.50

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Western blot analysis of various lysates, using CD3G antibody (STJ11103929) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Anti-CD3G antibody (23-116) (STJ11103929)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Flow cytometry: 1X10^6 Raji cells (negative control, left) and Jurkat cells (right) were intracellularly-stained with CD3G Rabbit monoclonal antibody (STJ11103551, 2. 5 Mu g/mL orange line) or ABflo® 488 Rabbit IgG isotype control ( 2. 5 Mu g/mL, blue line). Non-fluorescently stained cells were used as blank control (red line).

Anti-CD3G antibody (83-182) [S1MR] (STJ11103551)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/FC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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STJ73095 (0. 3µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CD3G antibody (Internal) (STJ73095)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CD3G antibody (STJ192944)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-CD3G antibody [SRM13441] (STJ11108171)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

£0.00

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Anti-CD3H antibody [SRM14537] (STJ11109267)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CD4 (PTR2055) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: JurkatPredicted band size: 51kDaObserved band size: 51kDa

Anti-CD4 antibody (1-100) [PTR2055] (STJA0006231)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£49.00 - £261.00

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Immunofluorescence analysis of Jurkat cells using CD4 Rabbit polyclonal antibody (STJ114878) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-CD4 antibody (26-200) (STJ114878)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of paraffin-embedded mouse spleen using CD4 Rabbit polyclonal antibody (STJ114879) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CD4 antibody (26-200) (STJ114879)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of THP-1 cells using CD4 Rabbit polyclonal antibody (STJ11105070) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-CD4 antibody (27-394 aa) (STJ11105070)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Western blot with Anti-CD4 Antibody (STJ500468) with peptide on at 1:500 dilution in buffer.

Anti-CD4 antibody (270-320) (STJ500468)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse

£427.50

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Anti-CD4 antibody (270-320) {Biotin} (STJ500470)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse

£528.50

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Anti-CD4 antibody (270-320) {FITC} (STJ500471)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse

£528.50

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Western blot analysis of various cells using CD4 Polyclonal Antibody diluted at 1：500

Anti-CD4 antibody (401-450 aa) (STJ92127)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunohistochemistry of mouse spleen with Anti-CD4 primary antibody (STJ500469) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-CD4 antibody (437-458) (STJ500469)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£427.50

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Anti-CD4 antibody (437-458) {Biotin} (STJ500472)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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