• Immunohistochemistry analysis of CCDC124 in paraffin-embedded mouse brain using CCDC124 Rabbit polyclonal antibody (STJ116510) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of CCDC124 in paraffin-embedded mouse testis using CCDC124 Rabbit polyclonal antibody (STJ116510) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of CCDC124 in paraffin-embedded human breast cancer using CCDC124 Rabbit polyclonal antibody (STJ116510) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of CCDC124 in paraffin-embedded human colon using CCDC124 Rabbit polyclonal antibody (STJ116510) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using CCDC124 Rabbit polyclonal antibody (STJ116510) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CCDC124 antibody (1-223) (STJ116510)

SKU:
STJ116510

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CCDC124 (1-223) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CCDC124
Gene ID: 115098
Uniprot ID: CC124_HUMAN
Immunogen Region: 1-223
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-223 of human CCDC124 (NP_612451.1).
Immunogen Sequence: MPKKFQGENTKSAAARARRA EAKAAADAKKQKELEDAYWK DDDKHVMRKEQRKEEKEKRR LDQLERKKETQRLLEEEDSK LKGGKAPRVATSSKVTRAQI EDTLRRDHQLREAPDTAEKA KSHLEVPLEENVNRRVLEEG SVEARTIEDAIAVLSVAEEA ADRHPERRMRAAFTAFEEAQ LPRLKQENPNMRLSQLKQLL KKEWLRSPDNPMNQRAVPFN APK
Tissue Specificity Ubiquitously expressed.
Function Ribosome-binding protein involved in ribosome hibernation: associates with translationally inactive ribosomes and stabilizes the nonrotated conformation of the 80S ribosome, thereby promoting ribosome preservation and storage. Also required for proper progression of late cytokinetic stages.
Protein Name Coiled-Coil Domain-Containing Protein 124
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Midbody
Colocalizes With Gamma-Tubulin At Interphase
Prophase
Metaphase
And Anaphase
Relocates From Centrosome To Midbody At Telophase
Alternative Antibody Names Anti-Coiled-Coil Domain-Containing Protein 124 antibody
Anti-CCDC124 antibody

Information sourced from Uniprot.org

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