• Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells using 3 Mu g CCAR1 antibody (STJ115556). Western blot was performed from the immunoprecipitate using CCAR1 antibody (STJ115556) at a dilution of 1:500.
  • Western blot analysis of various lysates using CCAR1 Rabbit polyclonal antibody (STJ115556) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-CCAR1 antibody (1-200) (STJ115556)

SKU:
STJ115556

£169.50 - £449.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CCAR1 (1-200) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CCAR1
Gene ID: 55749
Uniprot ID: CCAR1_HUMAN
Immunogen Region: 1-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human CCAR1 (NP_060707.2).
Immunogen Sequence: MAQFGGQKNPPWATQFTATA VSQPAALGVQQPSLLGASPT IYTQQTALAAAGLTTQTPAN YQLTQTAALQQQAAAAAAAL QQQYSQPQQALYSVQQQLQQ PQQTLLTQPAVALPTSLSLS TPQPTAQITVSYPTPRSSQQ QTQPQKQRVFTGVVTKLHDT FGFVDEDVFFQLSAVKGKTP QVGDRVLVEATYNPNMPFKW
Tissue Specificity Expressed in various epithelial cancer cell lines, including breast, colon, prostate, pancreatic and leukemia. Expression is regulated by growth factors.
Function Associates with components of the Mediator and p160 coactivator complexes that play a role as intermediaries transducing regulatory signals from upstream transcriptional activator proteins to basal transcription machinery at the core promoter. Recruited to endogenous nuclear receptor target genes in response to the appropriate hormone. Also functions as a p53 coactivator. May thus play an important role in transcriptional regulation. May be involved in apoptosis signaling in the presence of the reinoid CD437. Apoptosis induction involves sequestration of 14-3-3 protein(s) and mediated altered expression of multiple cell cycle regulatory genes including MYC, CCNB1 and CDKN1A. Plays a role in cell cycle progression and/or cell proliferation. In association with CALCOCO1 enhances GATA1- and MED1-mediated transcriptional activation from the gamma-globin promoter during erythroid differentiation of K562 erythroleukemia cells. Can act as a both a coactivator and corepressor of AR-mediated transcription. Contributes to chromatin looping and AR transcription complex assembly by stabilizing AR-GATA2 association on chromatin and facilitating MED1 and RNA polymerase II recruitment to AR-binding sites. May play an important role in the growth and tumorigenesis of prostate cancer cells.
Protein Name Cell Division Cycle And Apoptosis Regulator Protein 1
Cell Cycle And Apoptosis Regulatory Protein 1
Carp-1
Death Inducer With Sap Domain
Database Links Reactome: R-HSA-72163
Cellular Localisation Cytoplasm
Perinuclear Region
Alternative Antibody Names Anti-Cell Division Cycle And Apoptosis Regulator Protein 1 antibody
Anti-Cell Cycle And Apoptosis Regulatory Protein 1 antibody
Anti-Carp-1 antibody
Anti-Death Inducer With Sap Domain antibody
Anti-CCAR1 antibody
Anti-CARP1 antibody
Anti-DIS antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance