Immunohistochemistry Antibodies | St John's Laboratory

Western blot analysis of lysates from 1) Hela, 2) 293, 3)mouse braincells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） Actin Beta monoclonal antibody (5B7) (cat: (STJ96930) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-HIF1A antibody (328-377 aa) (STJ93498)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from HeLa cells, using HIF1 Alpha Rabbit pAb (STJ113863) at 1:1000 dilution. HeLa cells were treated by Cobalt chloride (0. 1 mM) at 37 °C for 4 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-HIF1A antibody (670-826) (STJ113863)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis using HIF-1 Alpha monoclonal antibody against Cos7 (1) , HeLa (2) , Jurkat (3) , RAJI (4) and NIH/3T3 (5) cell lysate.

Anti-HIF1A antibody [1A3] (STJ98135)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

£149.00 - £360.00

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Western blot analysis of 1) HepG2 Cell, 2) HepG2 Treated with CoCl2 cell lysate using HIF-1α mouse Monoclonal monoclonal antibody diluted at 1:2,000.

Anti-HIF1A antibody [Mix] (STJ196669)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£180.00 - £261.00

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Immunoprecipitation analysis of 200 Mu g extracts of 293T cells using 1 Mu g HIF1AN/FIH1 antibody (STJ27419). Western blot was performed from the immunoprecipitate using HIF1AN/FIH1 antibody (STJ27419) at a dilution of 1:1000.

Anti-HIF1AN antibody (1-349) (STJ27419)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-HIF1AN/FIH-1 antibody [SRM14979] (STJ11109709)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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STJ73323-D2 (4µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Anti-HIF3A antibody (N-Term) (STJ73323)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC

Reactivity :

Human/Mouse/Rat

£312.50

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Anti-HIG2 antibody (20-63 aa) (STJ13101234)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Immunofluorescence analysis of U-2 OS cells using HIGD1A Rabbit polyclonal antibody (STJ116792) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HIGD1A antibody (1-93) (STJ116792)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

£110.50 - £505.50

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Immunohistochemical analysis of paraffin-embedded human colon cancer. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-HIGD1C antibody (29-79 aa) (STJ196215)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of HILPDA in paraffin-embedded Mouse liver using HILPDA Rabbit polyclonal antibody (STJ11100178) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-HILPDA antibody (1-63) (STJ11100178)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemical analysis of paraffin-embedded Human prostate cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-HINT1 antibody (71-120 aa) (STJ93501)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-HINT1 antibody [SRM13099] (STJ11107829)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-HINT2 antibody (STJA0004739)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human/Mouse

£139.50 - £403.50

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Anti-HINT2 antibody (STJA0004740)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ELISA

Reactivity :

Human/Mouse

£139.50 - £403.50

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Anti-HINT2 antibody (STJA0004741)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human

£139.50 - £403.50

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STJ70643 (1µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-HIP14/ZDHHC17 antibody (C-Term) (STJ70643)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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STJ70654 (0. 1µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-HIP14L/ZDHHC13 antibody (C-Term) (STJ70654)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat

£312.50

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STJ70291 staining (1. 5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Anti-HIP2 antibody (C-Term) (STJ70291)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Dog/Pig/Cow

£312.50

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Western blot analysis of various cells using HIPK4 Polyclonal Antibody

Anti-HIPK4 antibody (511-560 aa) (STJ93502)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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STJ71890 (1µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-HIPPI antibody (Internal) (STJ71890)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Cow

£312.50

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Western blot analysis of various lysates using [KO Validated] HIRA Rabbit pAb (STJ110759) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-HIRA antibody (250-550) (STJ110759)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£126.50 - £618.50

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Immunohistochemistry analysis of paraffin-embedded human tonsil tissue, using HIRA Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-HIRA antibody (521-570 aa) (STJ93503)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of U2OS cells using HIRIP3 antibody (STJ29094).

Anti-HIRIP3 antibody (417-556) (STJ29094)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-HIS-Tag antibody (HHHHHH) (STJ13101213)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

HIS-Tag

£331.50

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Anti-HIS-Tag antibody (HHHHHH) (STJ13101214)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

HIS-Tag

£331.50

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Anti-HIS-Tag antibody (HHHHHH) (STJ13101218)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

HIS-Tag

£331.50

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Anti-HIS-Tag antibody (HHHHHH) (STJ13101220)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

HIS-Tag

£331.50

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Anti-HIST1H1D antibody (STJA0004374)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human/Mouse

£139.50 - £403.50

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Western blot analysis of extracts of various cell lines, using Histone H2A rabbit monoclonal antibody (STJ11101928) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HIST1H2AE antibody (1-100) [S8MR] (STJ11101928)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat/Other

£118.50 - £521.50

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Immunohistochemistry analysis of Hydroxyl-Histone H2A-Y39 in paraffin-embedded human esophagus tissue using Hydroxyl-Histone H2A-Y39 Rabbit monoclonal antibody (STJ11102162) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-HIST1H2AE-Y39 antibody (Around Y39) [S2MR] (STJ11102162)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Other

£118.50 - £521.50

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Anti-HIST1H2AH antibody (STJA0004677)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ELISA

Reactivity :

Human/Mouse

£140.50 - £415.50

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Anti-HIST1H2AH antibody (STJA0004678)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ELISA

Reactivity :

Human/Mouse

£139.50 - £403.50

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Western blot analysis of various lysates using HIST1H2BG Rabbit pAb (STJ11107549) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-HIST1H2BG antibody (1-126 aa) (STJ11107549)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£110.50 - £505.50

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STJ72900 (0. 1µg/ml) staining of A549 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-histamine H3 receptor antibody (Internal) (STJ72900)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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STJ71130 (0. 5µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Histamine receptor H1 (internal) antibody (Internal) (STJ71130)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human

£312.50

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STJ70765 (4µg/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Anti-Histamine Receptor H1 antibody (C-Term) (STJ70765)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC/IF/FC

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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Anti-Histatin 1 antibody (10-40 aa) (STJ13101210)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Immunohistochemistry analysis of Histone H1. 0 in paraffin-embedded human thyroid cancer using Histone H1. 0 Rabbit monoclonal antibody (STJ11102045) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-Histone H1 antibody (1-100) [S5MR] (STJ11102045)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Other

£118.50 - £521.50

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Immunohistochemistry analysis of paraffin-embedded rat brain using [KO Validated] Histone H1. 0 Rabbit polyclonal antibody (STJ23903) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Histone H1 antibody (1-105) (STJ23903)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunofluorescence analysis of Hela cell. 1, Histone H1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Alpha-SMA monoclonal antibody (6A12) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Histone H1 antibody (1-50 aa) (STJ93515)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of Histone H1t in paraffin-embedded rat lung using Histone H1t Rabbit polyclonal antibody (STJ11100449) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Histone H1 antibody (150-207) (STJ11100449)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-Histone H1 antibody (STJ401533)

Clonality :

Polyclonal

Host :

Sheep

Applications :

WB/IHC/IP

Reactivity :

Histone H1

£0.00

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Anti-Histone H1 antibody [AE-4] (STJ16100269)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IF/IHC

Reactivity :

Human/Mouse/Rat

£308.50 - £1,349.50

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Immunocytochemistry analysis of Histone H1. 3 (green) in Hela using Histone H1. 3 antibody, and DAPI (blue)

Anti-Histone H1.3 antibody (5-17 aa) [R09-8H7] (STJA0011419)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

£246.50

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Anti-Histone H12 antibody [N287/43] (STJA0021272)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

£212.50 - £1,184.50

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Anti-Histone H12 antibody [N287/65] (STJA0021273)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

£212.50 - £1,184.50

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Anti-Histone H18 antibody [N287/67] (STJA0021175)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

£212.50 - £1,184.50

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