• Western blot analysis of extracts of various cell lines, using Fatty Acid Synthase (FASN) antibody (STJ23631) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of various lysates, using Fatty Acid Synthase (FASN) rabbit polyclonal antibody antibody (STJ23631) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 5s.
  • Western blot analysis of Rat liver, using Fatty Acid Synthase (FASN) rabbit polyclonal antibody antibody (STJ23631) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 5s.
  • Western blot analysis of extracts from wild type (WT) and Fatty Acid Synthase (FASN) rabbit polyclonal antibody knockout (KO) HeLa cells, using Fatty Acid Synthase (FASN) rabbit polyclonal antibody antibody (STJ23631) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 5s.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using Fatty Acid Synthase (FASN) antibody (STJ23631) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of HeLa cells using Fatty Acid Synthase (FASN) rabbit polyclonal antibody (STJ23631) at a dilution of 1:250 (40x lens). Secondary antibody:Cy3 Goat Anti-rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-FASN antibody (2221-2511) (STJ23631)

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STJ23631

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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Fatty Acid Synthase (2221-2511) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FASN
Gene ID: 2194
Uniprot ID: FAS_HUMAN
Immunogen Region: 2221-2511
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 2221-2511 of human Fatty Acid Synthase (FASN) (NP_004095.4).
Immunogen Sequence: SLLVNPEGPTLMRLNSVQSS ERPLFLVHPIEGSTTVFHSL ASRLSIPTYGLQCTRAAPLD SIHSLAAYYIDCIRQVQPEG PYRVAGYSYGACVAFEMCSQ LQAQQSPAPTHNSLFLFDGS PTYVLAYTQSYRAKLTPGCE AEAETEAICFFVQQFTDMEH NRVLEALLPLKGLEERVAAA VDLIIKSHQGLDRQELSFAA RSFYYKLRAAEQYTPKAKYH GNVMLLRAKTGGAYGEDLG
Tissue Specificity Ubiquitous. Prominent expression in brain, lung, liver and mammary gland.
Post Translational Modifications S-nitrosylation of Fatty acid synthase at cysteine residues Cys-1471 or Cys-2091 is important for the enzyme dimerization. In adipocytes, S-nitrosylation of Fatty acid synthase occurs under physiological conditions and gradually increases during adipogenesis.
Function Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein (ACP) domain. (Microbial infection) Fatty acid synthetase activity is required for SARS coronavirus-2/SARS-CoV-2 replication.
Protein Name Fatty Acid Synthase
Type I Fatty Acid Synthase Includes - Acyl-Carrier-Protein S-Acetyltransferase - Acyl-Carrier-Protein S-Malonyltransferase - 3-Oxoacyl-Acyl-Carrier-Protein Synthase - 3-Oxoacyl-Acyl-Carrier-Protein Reductase - 3-Hydroxyacyl-Acyl-Carrier-Protein Dehydratase - Enoyl-Acyl-Carrier-Protein Reductase - Acyl-Acyl-Carrier-Protein Hydrolase
Database Links Reactome: R-HSA-163765
Reactome: R-HSA-199220
Reactome: R-HSA-2426168
Reactome: R-HSA-75105
Reactome: R-HSA-9029558
Cellular Localisation Cytoplasm
Melanosome
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-Fatty Acid Synthase antibody
Anti-Type I Fatty Acid Synthase Includes - Acyl-Carrier-Protein S-Acetyltransferase - Acyl-Carrier-Protein S-Malonyltransferase - 3-Oxoacyl-Acyl-Carrier-Protein Synthase - 3-Oxoacyl-Acyl-Carrier-Protein Reductase - 3-Hydroxyacyl-Acyl-Carrier-Protein Dehydratase - Enoyl-Acyl-Carrier-Protein Reductase - Acyl-Acyl-Carrier-Protein Hydrolase antibody
Anti-FASN antibody
Anti-FAS antibody

Information sourced from Uniprot.org

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