Anti-FBL2/FBXL2 antibody (N-Term) (STJ70401)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-FBL2/FBXL2 (N-Term) is suitable for use in ELISA, Immunohistochemistry and Immunofluorescence research applications. |
| Applications | Pep-ELISA/IHC/IF |
| Host | Goat |
| Reactivity | Human/Rat/Dog/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Preliminary testing showed a band at approx 48kDa in Human Kidney lysate after 0.1µg/ml antibody staining (calculated MW of 47.1kDa according to NP_036289.2). Primary incubation 1 hou |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | FBXL2 |
| Gene ID | 25827 |
| Uniprot ID | FBXL2_HUMAN |
| Accession Number | NP_036289.2 |
| Immunogen Region | N-Term |
| Immunogen Sequence | VFSNNDEGLINKK |
Additional Info
| Post Translational Modifications | Phosphorylated by GSK-beta (GSK3B), promoting recognition by FBXO3, leading to its ubiquitination by the SCF(FBXO3) complex. Ubiquitinated at Lys-201 by the SCF(FBXO3) complex in response to lipopolysaccharide (LPS), leading to its degradation by the proteasome. |
| Function | Calcium-activated substrate recognition component of the SCF (SKP1-cullin-F-box protein) E3 ubiquitin-protein ligase complex, SCF(FBXL2), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Unlike many F-box proteins, FBXL2 does not seem to target phosphodegron within its substrates but rather calmodulin-binding motifs and is thereby antagonized by calmodulin. This is the case for the cyclins CCND2 and CCND3 which polyubiquitination and subsequent degradation are inhibited by calmodulin. Through CCND2 and CCND3 degradation induces cell-cycle arrest in G(0). SCF(FBXL2) also mediates PIK3R2 ubiquitination and proteasomal degradation thereby regulating phosphatidylinositol 3-kinase signaling and autophagy. PCYT1A monoubiquitination by SCF(FBXL2) and subsequent degradation regulates synthesis of phosphatidylcholine, which is utilized for formation of membranes and of pulmonary surfactant. The SCF(FBXL2) complex acts as a regulator of inflammation by mediating ubiquitination and degradation of TRAF proteins (TRAF1, TRAF2, TRAF3, TRAF4, TRAF5 and TRAF6). The SCF(FBXL2) complex acts as a negative regulator of the NLRP3 inflammasome by mediating ubiquitination and degradation of NLRP3. |
| Protein Name | F-Box/Lrr-Repeat Protein 2F-Box And Leucine-Rich Repeat Protein 2F-Box Protein Fbl2/Fbl3 |
| Cellular Localisation | MembraneLipid-Anchor |
| Alternative Antibody Names | Anti-F-Box/Lrr-Repeat Protein 2 antibodyAnti-F-Box And Leucine-Rich Repeat Protein 2 antibodyAnti-F-Box Protein Fbl2/Fbl3 antibodyAnti-FBXL2 antibodyAnti-FBL2 antibodyAnti-FBL3 antibody |
Information sourced from Uniprot.org