Anti-FAS/CD95 antibody (Internal) (STJ72023)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-FAS/CD95 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/WB/IHC |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 37kDa band observed in lysates of cell line MOLT4 (calculated MW of 37.7kDa according to NP_000034.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | FAS |
| Gene ID | 355 |
| Uniprot ID | TNR6_HUMAN |
| Accession Number | NP_000034.1; NP_690610.1; NP_690611.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | KTCRKHRKENQGSH |
| Specificity | This antibody is expected to recognize reported isoforms 1 (NP_000034.1) , 2 (NP_690610.1) and 3 (NP_690611.1). |
Additional Info
| Post Translational Modifications | (Microbial infection) Glycosylated at Arg-250 by enteropathogenic E.coli protein NleB1: arginine GlcNAcylation prevents homotypic/heterotypic death domain interactions. Palmitoylated. Palmitoylation by ZDHHC7 prevents the lysosomal degradation of FAS regulating its expression at the plasma membrane. N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. |
| Function | Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase CASP8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs CASP8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). |
| Protein Name | Tumor Necrosis Factor Receptor Superfamily Member 6Apo-1 AntigenApoptosis-Mediating Surface Antigen FasFaslg ReceptorCd Antigen Cd95 |
| Database Links | Reactome: R-HSA-140534Reactome: R-HSA-3371378Reactome: R-HSA-5213460Reactome: R-HSA-5218900Reactome: R-HSA-6803211Reactome: R-HSA-69416Reactome: R-HSA-75157 |
| Cellular Localisation | Isoform 1: Cell MembraneSingle-Pass Type I Membrane ProteinMembrane RaftIsoform 2: SecretedIsoform 3: SecretedIsoform 4: SecretedIsoform 5: SecretedIsoform 6: Secreted |
| Alternative Antibody Names | Anti-Tumor Necrosis Factor Receptor Superfamily Member 6 antibodyAnti-Apo-1 Antigen antibodyAnti-Apoptosis-Mediating Surface Antigen Fas antibodyAnti-Faslg Receptor antibodyAnti-Cd Antigen Cd95 antibodyAnti-FAS antibodyAnti-APT1 antibodyAnti-FAS1 antibodyAnti-TNFRSF6 antibody |
Information sourced from Uniprot.org