Anti-FASN antibody (2400-2500) [S8MR] (STJ11101588)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal FASN (2400-2500) antibody for use in WB, IHC-P, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S8MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:2000IHC-P:1:500-1:2000IF/ICC:1:100-1:800IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your sp |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | FASN |
| Gene ID | 2194 |
| Uniprot ID | FAS_HUMAN |
| Immunogen Region | 2400-2500 |
| Immunogen Sequence | AVDLIIKSHQGLDRQELSFA ARSFYYKLRAAEQYTPKAKY HGNVMLLRAKTGGAYGEDLG ADYNLSQVCDGKVSVHVIEG DHRTLLEGSGLESIISIIHS S |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 2400-2500 of human FASN (P49327). |
Additional Info
| Tissue Specificity | Ubiquitous. Prominent expression in brain, lung, liver and mammary gland. |
| Post Translational Modifications | S-nitrosylation of Fatty acid synthase at cysteine residues Cys-1471 or Cys-2091 is important for the enzyme dimerization. In adipocytes, S-nitrosylation of Fatty acid synthase occurs under physiological conditions and gradually increases during adipogenesis. |
| Function | Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein (ACP) domain. (Microbial infection) Fatty acid synthetase activity is required for SARS coronavirus-2/SARS-CoV-2 replication. |
| Protein Name | Fatty Acid SynthaseType I Fatty Acid Synthase Includes - Acyl-Carrier-Protein S-Acetyltransferase - Acyl-Carrier-Protein S-Malonyltransferase - 3-Oxoacyl-Acyl-Carrier-Protein Synthase - 3-Oxoacyl-Acyl-Carrier-Protein Reductase - 3-Hydroxyacyl-Acyl-Carrier-Protein Dehydratase - Enoyl-Acyl-Carrier-Protein Reductase - Acyl-Acyl-Carrier-Protein Hydrolase |
| Database Links | Reactome: R-HSA-163765Reactome: R-HSA-199220Reactome: R-HSA-2426168Reactome: R-HSA-75105Reactome: R-HSA-9029558 |
| Cellular Localisation | CytoplasmMelanosomeIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv |
| Alternative Antibody Names | Anti-Fatty Acid Synthase antibodyAnti-Type I Fatty Acid Synthase Includes - Acyl-Carrier-Protein S-Acetyltransferase - Acyl-Carrier-Protein S-Malonyltransferase - 3-Oxoacyl-Acyl-Carrier-Protein Synthase - 3-Oxoacyl-Acyl-Carrier-Protein Reductase - 3-Hydroxyacyl-Acyl-Carrier-Protein Dehydratase - Enoyl-Acyl-Carrier-Protein Reductase - Acyl-Acyl-Carrier-Protein Hydrolase antibodyAnti-FASN antibodyAnti-FAS antibody |
Information sourced from Uniprot.org